Optimization of Ammonia Fiber Expansion (AFEX) Pretreatment and Enzymatic Hydrolysis ofMiscanthus x giganteusto Fermentable Sugars

2007 ◽  
Vol 23 (4) ◽  
pp. 846-850 ◽  
Author(s):  
Hannah K. Murnen ◽  
Venkatesh Balan ◽  
Shishir P. S. Chundawat ◽  
Bryan Bals ◽  
Leonardo da Costa Sousa ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Fermentable Sugars ◽  
Ammonia Fiber Expansion ◽  
Afex Pretreatment

Optimization of Ammonia Fiber Expansion (AFEX) Pretreatment and Enzymatic Hydrolysis of Miscanthus x giganteus to Fermentable Sugars

2007 ◽  
Vol 23 (4) ◽  
pp. 846-850 ◽  
Author(s):  
H.K. Murnen ◽  
V. Balan ◽  
S.P.S. Chundawat ◽  
B. Bals ◽  
L. daCostaSousa ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Fermentable Sugars ◽  
Miscanthus X Giganteus ◽  
Ammonia Fiber Expansion ◽  
Afex Pretreatment ◽  
Hydrolysis Of

scholarly journals Ammonia Fiber Expansion (Afex) Treatment of Wheat Straw for Production of Bioethanol

2021 ◽  
Author(s):  
Seyed Farshidreza Emam
Keyword(s):  
Enzymatic Hydrolysis ◽  
Moisture Content ◽  
Wheat Straw ◽  
Residence Time ◽  
Dry Weight ◽  
Maximum Efficiency ◽  
Lignocellulosic Materials ◽  
Ammonia Fiber Expansion ◽  
Afex Pretreatment ◽  
Hydrolysis Of

Ammonia Fiber Expansion (AFEX) treatment is a technique that is able to enhance the enzymatic hydrolysis yield of lignocellulosic materials. In this technique, lignocellulosic materials are treated by liquid ammonia under pressure followed by rapid release of pressure that expands the fiber structure and increases enzyme access to lignocellulose polysaccharides. However, the AFEX treatment variables such as the mass ratio of ammonia to lignocellulosic biomass, moisture of lignocellulose (moisture content of biomass), temperature, and residence time need to be evaluated to find the maximum efficiency of this treatment. The efficiency of the AFEX pretreatment was quantified by the yield of released sugars during enzymatic hydrolysis of the AFEX-treated wheat straw. The optimal treatment conditions for wheat straw were found to be: ammonia-to-wheat straw ratio, 1:1; temperature, 95°C; moisture content of wheat straw, 70% (dry weight basis); and residence time, 5 minutes. Under these conditions, almost 89% of the theoretical sugars were released by enzymatic hydrolysis of the AFEX-treated wheat straw. The enzymatic hydrolysis results showed the significance of AFEX pretreatment of wheat straw when compared to untreated wheat straw with released sugars yield of only 26 %.

scholarly journals Ammonia Fiber Expansion (Afex) Treatment of Wheat Straw for Production of Bioethanol

2021 ◽  
Author(s):  
Seyed Farshidreza Emam
Keyword(s):  
Enzymatic Hydrolysis ◽  
Moisture Content ◽  
Wheat Straw ◽  
Residence Time ◽  
Dry Weight ◽  
Maximum Efficiency ◽  
Lignocellulosic Materials ◽  
Ammonia Fiber Expansion ◽  
Afex Pretreatment ◽  
Hydrolysis Of

Ammonia Fiber Expansion (AFEX) treatment is a technique that is able to enhance the enzymatic hydrolysis yield of lignocellulosic materials. In this technique, lignocellulosic materials are treated by liquid ammonia under pressure followed by rapid release of pressure that expands the fiber structure and increases enzyme access to lignocellulose polysaccharides. However, the AFEX treatment variables such as the mass ratio of ammonia to lignocellulosic biomass, moisture of lignocellulose (moisture content of biomass), temperature, and residence time need to be evaluated to find the maximum efficiency of this treatment. The efficiency of the AFEX pretreatment was quantified by the yield of released sugars during enzymatic hydrolysis of the AFEX-treated wheat straw. The optimal treatment conditions for wheat straw were found to be: ammonia-to-wheat straw ratio, 1:1; temperature, 95°C; moisture content of wheat straw, 70% (dry weight basis); and residence time, 5 minutes. Under these conditions, almost 89% of the theoretical sugars were released by enzymatic hydrolysis of the AFEX-treated wheat straw. The enzymatic hydrolysis results showed the significance of AFEX pretreatment of wheat straw when compared to untreated wheat straw with released sugars yield of only 26 %.

scholarly journals Organosolv pretreatment assisted by carbocation scavenger to mitigate surface barrier effect of lignin for improving biomass saccharification and utilization

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Qiulu Chu ◽  
Wenyao Tong ◽  
Jianqiang Chen ◽  
Shufang Wu ◽  
Yongcan Jin ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Surface Area ◽  
Cellulose Hydrolysis ◽  
Syringic Acid ◽  
Fermentable Sugars ◽  
Surface Barrier ◽  
Textile Dye ◽  
Barrier Effect ◽  
Biomass Saccharification ◽  
Carbocation Scavenger

Abstract Background Ethanol organosolv (EOS) pretreatment is one of the most efficient methods for boosting biomass saccharification as it can achieve an efficient fractionation of three major constituents in lignocellulose. However, lignin repolymerization often occurs in acid EOS pretreatment, which impairs subsequent enzymatic hydrolysis. This study investigated acid EOS pretreatment assisted by carbocation scavenger (2-naphthol, 2-naphthol-7-sulfonate, mannitol and syringic acid) to improve biomass fractionation, coproduction of fermentable sugars and lignin adsorbents. In addition, surface barrier effect of lignin on cellulose hydrolysis was isolated from unproductive binding effect of lignin, and the analyses of surface chemistry, surface morphology and surface area were carried out to reveal the lignin inhibition mitigating effect of various additives. Results Four different additives all helped mitigate lignin inhibition on cellulose hydrolysis in particular diminishing surface barrier effect, among which 2-naphthol-7-sulfonate showed the best performance in improving pretreatment efficacy, while mannitol and syringic acid could serve as novel green additives. Through the addition of 2-naphthol-7-sulfonate, selective lignin removal was increased up to 76%, while cellulose hydrolysis yield was improved by 85%. As a result, 35.78 kg cellulose and 16.63 kg hemicellulose from 100 kg poplar could be released and recovered as fermentable sugars, corresponding to a sugar yield of 78%. Moreover, 22.56 kg ethanol organosolv lignin and 17.53 kg enzymatic hydrolysis residue could be recovered as lignin adsorbents for textile dye removal, with the adsorption capacities of 45.87 and 103.09 mg g−1, respectively. Conclusions Results in this work indicated proper additives could give rise to the form of less repolymerized surface lignin, which would decrease the unproductive binding of cellulase enzymes to surface lignin. Besides, the supplementation of additives (NS, MT and SA) resulted in a simultaneously increased surface area and decreased lignin coverage. All these factors contributed to the diminished surface barrier effect of lignin, thereby improving the ease of enzymatic hydrolysis of cellulose. The biorefinery process based on acidic EOS pretreatment assisted by carbocation scavenger was proved to enable the coproduction of fermentable sugars and lignin adsorbents, allowing the holistic utilization of lignocellulosic biomass for a sustainable biorefinery. Graphic abstract

Tequilana weber Agave Bagasse Enzymatic Hydrolysis for the Production of Fermentable Sugars: Oxidative-Alkaline Pretreatment and Kinetic Modeling

2016 ◽  
Vol 9 (4) ◽  
pp. 998-1004 ◽  
Author(s):  
Ulises Velázquez-Valadez ◽  
Juan Carlos Farías-Sánchez ◽  
Alfonso Vargas-Santillán ◽  
Agustín Jaime Castro-Montoya
Keyword(s):  
Enzymatic Hydrolysis ◽  
Kinetic Modeling ◽  
Alkaline Pretreatment ◽  
Fermentable Sugars

Enzymatic hydrolysis of aspen biomass into fermentable sugars by using lignocellulases from Armillaria gemina

2013 ◽  
Vol 133 ◽  
pp. 307-314 ◽  
Author(s):  
Sujit Sadashiv Jagtap ◽  
Saurabh Sudha Dhiman ◽  
Tae-Su Kim ◽  
Jinglin Li ◽  
Jung-Kul Lee ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Fermentable Sugars ◽  
Hydrolysis Of
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