Carbon Flux Distributions at the Glucose 6-Phosphate Branch Point in Corynebacterium glutamicum during Lysine Overproduction

1994 ◽  
Vol 10 (3) ◽  
pp. 327-334 ◽  
Author(s):  
Joseph J. Vallino ◽  
Gregory Stephanopoulos
Keyword(s):  
Corynebacterium Glutamicum ◽  
Branch Point ◽  
Carbon Flux ◽  
Lysine Overproduction

scholarly journals Carbon-flux distribution in the central metabolic pathways of Corynebacterium glutamicum during growth on fructose

1998 ◽  
Vol 254 (1) ◽  
pp. 96-102 ◽  
Author(s):  
Helene Dominguez ◽  
Catherine Rollin ◽  
Armel Guyonvarch ◽  
Jean-Luc Guerquin-Kern ◽  
Muriel Cocaign-Bousquet ◽  
...  
Keyword(s):  
Corynebacterium Glutamicum ◽  
Metabolic Pathways ◽  
Carbon Flux ◽  
Flux Distribution ◽  
Carbon Flux Distribution

Automatic Redirection of Carbon Flux between Glycolysis and Pentose Phosphate Pathway Using an Oxygen-Responsive Metabolic Switch in Corynebacterium glutamicum

2020 ◽  
Vol 9 (4) ◽  
pp. 814-826
Author(s):  
Shunsuke Kobayashi ◽  
Hideo Kawaguchi ◽  
Tomokazu Shirai ◽  
Kazuaki Ninomiya ◽  
Kenji Takahashi ◽  
...  
Keyword(s):  
Corynebacterium Glutamicum ◽  
Pentose Phosphate Pathway ◽  
Carbon Flux ◽  
Pentose Phosphate ◽  
Metabolic Switch

Co-Expression of Threonine Dehydratase and Acetolactate Synthase in Escherichia Coli for L-Isoleucine Production

2014 ◽  
Vol 989-994 ◽  
pp. 997-1002 ◽  
Author(s):  
Jian Wang ◽  
Jia Kai Sun ◽  
Qing Yang Xu
Keyword(s):  
Escherichia Coli ◽  
Corynebacterium Glutamicum ◽  
Carbon Flux ◽  
Batch Fermentation ◽  
Acetolactate Synthase ◽  
Fed Batch ◽  
Threonine Dehydratase ◽  
E Coli ◽  
Fed Batch Fermentation ◽  
Fermentation Period

Metabolic engineering ofCorynebacterium glutamicumhas sought to divert carbon into L-isoleucine. However, the fermentation period of this strain is long. TheC.glutamicumYILW strain (LeuL, AHVr, SGr, Leu-MEr) was previously derived by repeated compound mutagenesis which could accumulate 20.2 g/L L-isoleucine in a 5-L jar fermentor. Overexpression of the threonine dehydratase gene (ilvA) fromCorynebacterium glutamicumYILW and coexpression of threonine dehydratase and acetolactate synthase (ilvBN) from it were employed to divert carbon flux toward L-isoleucine. The strainE. coliTRFC with the expression ofilvA could accumulate L-isoleucine of 6.8 g/L without accumulation of any L-threonine by fed-batch fermentation in a 5-L jar fermentor. However, the production of L-isoleucine by the strainE.coliTRFC with the co-expression ofilvA andilvBN was decreased by 19.1%, and the production of L-valine was increased by 40% compared with that ofE. coliTRFC with the expression ofilvA.

scholarly journals Link between Phosphate Starvation and Glycogen Metabolism in Corynebacterium glutamicum, Revealed by Metabolomics

2010 ◽  
Vol 76 (20) ◽  
pp. 6910-6919 ◽  
Author(s):  
Han Min Woo ◽  
Stephan Noack ◽  
Gerd M. Seibold ◽  
Sabine Willbold ◽  
Bernhard J. Eikmanns ◽  
...  
Keyword(s):  
Stationary Phase ◽  
Corynebacterium Glutamicum ◽  
Carbon Flux ◽  
Glycogen Content ◽  
Glycogen Synthesis ◽  
Glycogen Metabolism ◽  
Metabolite Analysis ◽  
Glycogen Level ◽  
Flux Balance ◽  
Balance Analysis

ABSTRACT In this study, we analyzed the influence of phosphate (Pi) limitation on the metabolism of Corynebacterium glutamicum. Metabolite analysis by gas chromatography-time-of-flight (GC-TOF) mass spectrometry of cells cultivated in glucose minimal medium revealed a greatly increased maltose level under Pi limitation. As maltose formation could be linked to glycogen metabolism, the cellular glycogen content was determined. Unlike in cells grown under Pi excess, the glycogen level in Pi-limited cells remained high in the stationary phase. Surprisingly, even acetate-grown cells, which do not form glycogen under Pi excess, did so under Pi limitation and also retained it in stationary phase. Expression of pgm and glgC, encoding the first two enzymes of glycogen synthesis, phosphoglucomutase and ADP-glucose pyrophosphorylase, was found to be increased 6- and 3-fold under Pi limitation, respectively. Increased glycogen synthesis together with a decreased glycogen degradation might be responsible for the altered glycogen metabolism. Independent from these experimental results, flux balance analysis suggested that an increased carbon flux to glycogen is a solution for C. glutamicum to adapt carbon metabolism to limited Pi concentrations.

scholarly journals Stable Expression of hom-1-thrB in Corynebacterium glutamicum and Its Effect on the Carbon Flux to Threonine and Related Amino Acids

1994 ◽  
Vol 60 (1) ◽  
pp. 126-132 ◽  
Author(s):  
Dieter J. Reinscheid ◽  
Wolfgang Kronemeyer ◽  
Lothar Eggeling ◽  
Bernhard J. Eikmanns ◽  
Hermann Sahm
Keyword(s):  
Amino Acids ◽  
Corynebacterium Glutamicum ◽  
Carbon Flux ◽  
Stable Expression

scholarly journals Modular control of multiple pathways of Corynebacterium glutamicum for 5-aminolevulinic acid production

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Fanglan Ge ◽  
Xiaokun Li ◽  
Qingrong Ge ◽  
Di Zhu ◽  
Wei Li ◽  
...  
Keyword(s):  
Corynebacterium Glutamicum ◽  
Carbon Flux ◽  
Large Scale ◽  
Aminolevulinic Acid ◽  
Protoporphyrin Ix ◽  
Tca Cycle ◽  
Strain Engineering ◽  
C4 Pathway ◽  
Encoding Gene ◽  
Glutamate Synthesis

Abstract5-aminolevulinic acid (ALA) has broad potential applications in the medical, agricultural and food industries. Several strategies have been implemented successfully to try to improve ALA synthesis. Nonetheless, the low yield has got in the way of large-scale bio-manufacture of 5-ALA. In this study, we explored strain engineering strategies for high‐level 5‐ALA production in Corynebacterium glutamicum F343 using the C4 pathway. Initially, the glutamate dehydrogenase-encoding gene gdhA was deleted to reduce glutamate yield. Then the C4 pathway was introduced in the gdhA mutant strain F2-A (∆gdhA + hemA), resulting in a 5-ALA yield of up to 3.2 g/L. Furthermore, the accumulations of downstream metabolites such as heme, porphobilinogen, and protoporphyrin IX, were decreased. After evaluating the mechanisms of this synthetic pathway by RNA-Seq, the results showed that genes involved in both the C5 pathway and heme pathways were down-regulated in strain F2-A (∆gdhA + hemA). Interestingly, upstream genes of succinyl-CoA in the tricarboxylic acid (TCA) cycle, such as icd, lpdA, were up-regulated, while its downstream genes, including sucC, sucD, sdhB, sdhA, sdhCD, were down-regulated. These changes amplify the sources of succinyl-CoA and reduce its expenditure, before pulling the carbon flux to produce 5-ALA. Furthermore, the down-regulation of most genes of the heme pathway could reduce the drainage of 5‐ALA, which further enhance its accumulation. To alleviate competition between glyoxylate and the TCA cycle, the isocitrate dehydrogenase-encoding gene aceA was also knocked out, resulting in 3.86 g/L of 5‐ALA. Finally, the fermentation conditions were optimized, resulting in a maximum 5-ALA yield of 5.6 g/L. Overall, the blocking of the glutamate synthesis pathway could be a powerful strategy to re-allocate the carbon flux to produce 5-ALA. It could also enable the efficient synthesis of other TCA derivatives in C. glutamicum.

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