Automatic Redirection of Carbon Flux between Glycolysis and Pentose Phosphate Pathway Using an Oxygen-Responsive Metabolic Switch in Corynebacterium glutamicum

2020 ◽  
Vol 9 (4) ◽  
pp. 814-826
Author(s):  
Shunsuke Kobayashi ◽  
Hideo Kawaguchi ◽  
Tomokazu Shirai ◽  
Kazuaki Ninomiya ◽  
Kenji Takahashi ◽  
...  
Keyword(s):  
Corynebacterium Glutamicum ◽  
Pentose Phosphate Pathway ◽  
Carbon Flux ◽  
Pentose Phosphate ◽  
Metabolic Switch

Optimal Ratio of Carbon Flux between Glycolysis and the Pentose Phosphate Pathway for Amino Acid Accumulation in Corynebacterium glutamicum

2020 ◽  
Vol 9 (7) ◽  
pp. 1615-1622
Author(s):  
Katsuki Murai ◽  
Daisuke Sasaki ◽  
Shunsuke Kobayashi ◽  
Akira Yamaguchi ◽  
Hiroto Uchikura ◽  
...  
Keyword(s):  
Amino Acid ◽  
Corynebacterium Glutamicum ◽  
Pentose Phosphate Pathway ◽  
Carbon Flux ◽  
Pentose Phosphate ◽  
Optimal Ratio ◽  
Acid Accumulation ◽  
Amino Acid Accumulation

scholarly journals Amplified Expression of Fructose 1,6-Bisphosphatase in Corynebacterium glutamicum Increases In Vivo Flux through the Pentose Phosphate Pathway and Lysine Production on Different Carbon Sources

2005 ◽  
Vol 71 (12) ◽  
pp. 8587-8596 ◽  
Author(s):  
Judith Becker ◽  
Corinna Klopprogge ◽  
Oskar Zelder ◽  
Elmar Heinzle ◽  
Christoph Wittmann
Keyword(s):  
Corynebacterium Glutamicum ◽  
Pentose Phosphate Pathway ◽  
Metabolic Flux ◽  
Carbon Sources ◽  
Pentose Phosphate ◽  
Metabolic Effects ◽  
Flux Analysis ◽  
Lysine Production ◽  
Fructose 1,6 Bisphosphatase

ABSTRACT The overexpression of fructose 1,6-bisphosphatase (FBPase) in Corynebacterium glutamicum leads to significant improvement of lysine production on different sugars. Amplified expression of FBPase via the promoter of the gene encoding elongation factor TU (EFTU) increased the lysine yield in the feedback-deregulated lysine-producing strain C. glutamicum lysCfbr by 40% on glucose and 30% on fructose or sucrose. Additionally formation of the by-products glycerol and dihydroxyacetone was significantly reduced in the PEFTUfbp mutant. As revealed by 13C metabolic flux analysis on glucose the overexpression of FBPase causes a redirection of carbon flux from glycolysis toward the pentose phosphate pathway (PPP) and thus leads to increased NADPH supply. Normalized to an uptake flux of glucose of 100%, the relative flux into the PPP was 56% for C. glutamicum lysCfbr PEFTUfbp and 46% for C. glutamicum lysCfb r . The flux for NADPH supply was 180% in the PEFTUfbp strain and only 146% in the parent strain. Amplification of FBPase increases the production of lysine via an increased supply of NADPH. Comparative studies with another mutant containing the sod promoter upstream of the fbp gene indicate that the expression level of FBPase relates to the extent of the metabolic effects. The overexpression of FBPase seems useful for starch- and molasses-based industrial lysine production with C. glutamicum. The redirection of flux toward the PPP should also be interesting for the production of other NADPH-demanding compounds as well as for products directly stemming from the PPP.

scholarly journals Enhanced Endogenous Amino Acids and Energy Metabolism Level for cAMP Biosynthesis by Arthrobacter sp. CCTCC 2013431 with Citrate as Cosubstrate

2021 ◽  
Author(s):  
Zhigang Li ◽  
Baofeng Chen ◽  
Yang Gu ◽  
Hai Tan ◽  
Zhonghua Zhang ◽  
...  
Keyword(s):  
Amino Acids ◽  
Energy Metabolism ◽  
Pentose Phosphate Pathway ◽  
Carbon Flux ◽  
Pentose Phosphate ◽  
Regulation Mechanism ◽  
Feedback Suppression ◽  
Metabolic Intensity ◽  
Glutamate Synthesis ◽  
Tricarboxylate Cycle

Abstract Objectives In our previous study, citrate was used as auxiliary energy substance for improving cAMP fermentation performance, however, the regulation mechanism of citrate on improved cAMP contents was not clear. To elucidate the regulation mechanism, cAMP fermentations with/without citrate addition were conducted in a 7 L fermentor using Arthrobacter sp. CCTCC 2013431 and assays on key enzymes activities, energy metabolism level, amino acids contents and peroxidation level were performed. Results With 3 g/L-broth sodium citrate added, cAMP concentration and conversion yield from glucose reached 4.34 g/L and 0.076 g/g which were improved by 30.7% and 29.8%, respectively, when compared with those of control. Citrate changed carbon flux distribution among different routes and more carbon flux was directed into pentose phosphate pathway beneficial to cAMP synthesis. Meanwhile, energy metabolism together with precursor amino acids levels were improved significantly owing to strengthened metabolic intensity of tricarboxylate cycle by exogenous citrate utilization which provided energy and substance basis for cAMP production. Moreover, higher glutamate synthesis and oxidative stress caused by citrate addition consumed excessive NADPH derived from pentose phosphate pathway by which feedback suppression for pentose phosphate pathway was relieved efficiently.Conclusion Citrate promoted cAMP fermentation production by Arthrobacter sp. CCTCC2013431 due to enhanced precursor amino acids, energy metabolism level and relieved feedback suppression for pentose phosphate pathway.

Hypoxia and oxygenation induce a metabolic switch between pentose phosphate pathway and glycolysis in glioma stem-like cells

2013 ◽  
Vol 126 (5) ◽  
pp. 763-780 ◽  
Author(s):  
Annegret Kathagen ◽  
Alexander Schulte ◽  
Gerd Balcke ◽  
Heidi S. Phillips ◽  
Tobias Martens ◽  
...  
Keyword(s):  
Pentose Phosphate Pathway ◽  
Pentose Phosphate ◽  
Metabolic Switch
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