scholarly journals Measurement and Control of Dissolved Carbon Dioxide in Mammalian Cell Culture Processes Using an in Situ Fiber Optic Chemical Sensor

2000 ◽  
Vol 16 (5) ◽  
pp. 769-774 ◽  
Author(s):  
R.N. Pattison ◽  
J. Swamy ◽  
B. Mendenhall ◽  
C. Hwang ◽  
B.T. Frohlich
Keyword(s):  
Carbon Dioxide ◽  
Cell Culture ◽  
Chemical Sensor ◽  
Fiber Optic ◽  
Mammalian Cell Culture ◽  
Dissolved Carbon ◽  
Dissolved Carbon Dioxide ◽  
And Control ◽  
Measurement And Control

scholarly journals Real‐time dissolved carbon dioxide monitoring I: Application of a novel in situ sensor for CO 2 monitoring and control

2020 ◽  
Vol 117 (4) ◽  
pp. 981-991 ◽  
Author(s):  
Viki R. Chopda ◽  
Timothy Holzberg ◽  
Xudong Ge ◽  
Brandon Folio ◽  
Michael Tolosa ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Real Time ◽  
Monitoring And Control ◽  
Dissolved Carbon ◽  
Dissolved Carbon Dioxide ◽  
Carbon Dioxide Monitoring ◽  
In Situ Sensor ◽  
And Control

scholarly journals In Situ Quantitative Raman Detection of Dissolved Carbon Dioxide and Sulfate in Deep-Sea High-Temperature Hydrothermal Vent Fluids

2018 ◽  
Vol 19 (6) ◽  
pp. 1809-1823 ◽  
Author(s):  
Lianfu Li ◽  
Xin Zhang ◽  
Zhendong Luan ◽  
Zengfeng Du ◽  
Shichuan Xi ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
High Temperature ◽  
Deep Sea ◽  
Hydrothermal Vent ◽  
Dissolved Carbon ◽  
Dissolved Carbon Dioxide

scholarly journals Novel Carbon Dioxide-Based Method for Accurate Determination of pH and pCO2 in Mammalian Cell Culture Processes

Processes ◽  
2020 ◽  
Vol 8 (5) ◽  
pp. 520
Author(s):  
Christian Klinger ◽  
Verena Trinkaus ◽  
Tobias Wallocha
Keyword(s):  
Carbon Dioxide ◽  
Cell Culture ◽  
Mammalian Cell ◽  
Mammalian Cell Culture ◽  
Accurate Determination ◽  
Reference Method ◽  
Dissolved Carbon ◽  
Non Invasive ◽  
Offline Measurement ◽  
Pco2 Monitoring

In mammalian cell culture, especially in pharmaceutical manufacturing, pH is a critical process parameter that has to be controlled as accurately as possible. Not only does pH directly affect cell culture performance, ensuring a comparable pH is also crucial for scaling and transfer of processes. A sample-based offline pH measurement is commonly used to ensure correct bioreactor pH probe signals after sterilization and as a detection measure for drifts of probe signals. However, the sample-based pH offline measurement does not necessarily deliver required accuracy. Offsets between bioreactor pH and sample pH heavily depend on equipment, local procedures and the offline measurement method that is used. This article adequately describes a novel, non-invasive method to determine pH and pCO2 in sterile bioreactors without the need to sample and measure offline. This method utilizes the chemical correlation between carbon dioxide in the gas phase, dissolved carbon dioxide, bicarbonate and dependent proton concentrations that directly affect the pH in carbonate buffered systems. The proposed carbon dioxide-based pH reference method thereby is able to accurately determine the true pH in the bioreactor without the need to sample. The proposed method is independent of scale and bioreactor configuration and does not depend on local procedures that may differ between sites, scales or operators. Applicability of the method for both stainless steel and single use bioreactors is shown. Furthermore, the very same principles are applicable for non-invasive, online pCO2 monitoring.

Impact of Dursban and Abate on Microbial Numbers and Some Chemical Properties of Standing Ponds

1975 ◽  
Vol 10 (1) ◽  
pp. 33-41 ◽  
Author(s):  
J. Butcher ◽  
M. Boyer ◽  
CD. Fowle
Keyword(s):  
Carbon Dioxide ◽  
Active Ingredient ◽  
Algal Blooms ◽  
Chemical Properties ◽  
Total Carbon ◽  
Dissolved Carbon ◽  
Photosynthetic Productivity ◽  
Dissolved Carbon Dioxide ◽  
Microbial Numbers

Abstract Eleven small ponds, lined with polyethylene, were used to assess the consequences of applications of *DursbanR at 0.004, 0.030, 0.100 and 1.000 ppm and AbateR at 0.025 and 0.100 ppm active ingredient. The treated ponds showed a more pronounced long-term increase in pH and dissolved oxygen and decreasing total and dissolved carbon dioxide in comparison with untreated ponds. Algal blooms were of longer duration in treated ponds than in controls. Total photosynthetic productivity was higher in treated ponds but bacterial numbers did not change significantly. Photosynthetic productivity was estimated by following the changes in total carbon dioxide.

Miniature Bioreactors for Rapid Bioprocess Development of Mammalian Cell Culture

2012 ◽  
Vol 59 (1) ◽  
Author(s):  
Mohd Helmi Sani ◽  
Frank Baganz
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Process Development ◽  
Mammalian Cell Culture ◽  
Small Scale ◽  
Cell Culture Process ◽  
Deep Wells ◽  
Working Volume ◽  
Culture Process ◽  
And Control

At present, there are a number of commercial small scale shaken systems available on the market with instrumented controllable microbioreactors such as Micro–24 Microreactor System (Pall Corporation, Port Washington, NY) and M2P Biolector, (M2P Labs GmbH, Aachen, Germany). The Micro–24 system is basically an orbital shaken 24–well plate that operates at working volume 3 – 7 mL with 24 independent reactors (deep wells, shaken and sparged) running simultaneously. Each reactor is designed as single use reactor that has the ability to continuously monitor and control the pH, DO and temperature. The reactor aeration is supplied by sparging air from gas feeds that can be controlled individually. Furthermore, pH can be controlled by gas sparging using either dilute ammonia or carbon dioxide directly into the culture medium through a membrane at the bottom of each reactor. Chen et al., (2009) evaluated the Micro–24 system for the mammalian cell culture process development and found the Micro–24 system is suitable as scaledown tool for cell culture application. The result showed that intra-well reproducibility, cell growth, metabolites profiles and protein titres were scalable with 2 L bioreactors.

scholarly journals In Situ Measurement and Control of the Fermi Level in Colloidal Nanocrystal Thin Films during Their Fabrication

2018 ◽  
Vol 9 (24) ◽  
pp. 7165-7172 ◽  
Author(s):  
Sebastian Volk ◽  
Nuri Yazdani ◽  
Olesya Yarema ◽  
Maksym Yarema ◽  
Deniz Bozyigit ◽  
...  
Keyword(s):  
Thin Films ◽  
Fermi Level ◽  
In Situ Measurement ◽  
And Control ◽  
Measurement And Control
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