Electrospinning of Highly Porous Scaffolds for Cartilage Regeneration

2008 ◽  
Vol 9 (3) ◽  
pp. 1044-1049 ◽  
Author(s):  
Anna Thorvaldsson ◽  
Hanna Stenhamre ◽  
Paul Gatenholm ◽  
Pernilla Walkenström
Keyword(s):  
Cartilage Regeneration ◽  
Porous Scaffolds ◽  
Highly Porous

scholarly journals Highly porous scaffolds of PEDOT:PSS for bone tissue engineering

2017 ◽  
Vol 62 ◽  
pp. 91-101 ◽  
Author(s):  
Anne Géraldine Guex ◽  
Jennifer L. Puetzer ◽  
Astrid Armgarth ◽  
Elena Littmann ◽  
Eleni Stavrinidou ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Porous Scaffolds ◽  
Highly Porous

scholarly journals Productionof poly(L-CO-D,L LacticAcid) porous fibers by electrospinning

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Nayara Maysa da Silva Carvalho ◽  
Bárbara E. Ciocca ◽  
Rubens Maciel Filho ◽  
Marcele Fonseca Passos ◽  
Maria Regina Wolf Maciel ◽  
...  
Keyword(s):  
Pore Formation ◽  
Scientific Community ◽  
Low Dielectric Constant ◽  
Porous Scaffolds ◽  
Low Density ◽  
Fiber Morphology ◽  
Low Dielectric ◽  
Mean Diameter ◽  
Porous Fibers ◽  
Highly Porous

The production of porous scaffolds has been widely investigated by the scientific community due to its suitability for tissue engineering. Among techniques that allow the fabrication of porous materials, electrospinning is appealing for being robust and versatile. This research investigated the pore formation in poly (L-co-D,L lactic acid) fibers obtained by conventional electrospinning and the influence of chloroform as a single solvent on fiber morphology. Random and highly porous fibers with a mean diameter of 2.373 ± 0.564 µm were collected. Chloroform affects the fiber morphology, mainly for its fast evaporation and low density of charges. The solvent on the surface evaporates quickly, and the low stretch of the jet does not help the polymer to reorganize over the length of the fiber, forming pores. In conclusion, the low dielectric constant and boiling point of chloroform induce pores formation along the PLDLA fibers.  

scholarly journals Novel whey protein isolate-based highly porous scaffolds modified with therapeutic ion-releasing bioactive glasses

2020 ◽  
Vol 261 ◽  
pp. 127115 ◽  
Author(s):  
Michal Dziadek ◽  
Timothy E.L. Douglas ◽  
Kinga Dziadek ◽  
Barbara Zagrajczuk ◽  
Andrada Serafim ◽  
...  
Keyword(s):  
Whey Protein ◽  
Whey Protein Isolate ◽  
Protein Isolate ◽  
Porous Scaffolds ◽  
Bioactive Glasses ◽  
Highly Porous

Fabrication of highly porous scaffolds for tissue engineering based on star-shaped functional poly(ε-caprolactone)

2010 ◽  
Vol 108 (3) ◽  
pp. 694-703 ◽  
Author(s):  
Stefan Theiler ◽  
Petra Mela ◽  
Stefanos E. Diamantouros ◽  
Stefan Jockenhoevel ◽  
Helmut Keul ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Porous Scaffolds ◽  
Highly Porous

scholarly journals Polycaprolactone Foam Functionalized With Chitosan Microparticles – a Suitable Scaffold for Cartilage Regeneration

2016 ◽  
pp. 121-131 ◽  
Author(s):  
E. FILOVÁ ◽  
B. JAKUBCOVÁ ◽  
I. DANILOVÁ ◽  
E. KUŽELOVÁ KOŠŤÁKOVÁ ◽  
T. JAROŠÍKOVÁ ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Cell Growth ◽  
Dna Content ◽  
Viscoelastic Properties ◽  
Cartilage Regeneration ◽  
Biomechanical Properties ◽  
Porous Scaffolds ◽  
Chitosan Microparticles ◽  
Salt Leaching ◽  
Cell Growth And Differentiation

For biodegradable porous scaffolds to have a potential application in cartilage regeneration, they should enable cell growth and differentiation and should have adequate mechanical properties. In this study, our aim was to prepare biocompatible scaffolds with improved biomechanical properties. To this end, we have developed foam scaffolds from poly-Ɛ-caprolactone (PCL) with incorporated chitosan microparticles. The scaffolds were prepared by a salt leaching technique from either 10 or 15 wt% PCL solutions containing 0, 10 and 20 wt% chitosan microparticles, where the same amount and size of NaCl was used as a porogen in all the cases. PCL scaffolds without and with low amounts of chitosan (0 and 10 wt% chitosan) showed higher DNA content than scaffolds with high amounts of chitosan during a 22-day experiment. 10 wt% PCL with 10 and 20 wt% chitosan showed significantly increased viscoelastic properties compared to 15 wt% PCL scaffolds with 0 and 10 wt% chitosan. Thus, 10 wt% PCL scaffolds with 0 wt% and 10 wt% chitosan are potential scaffolds for cartilage regeneration.

scholarly journals Synthesis and Investigation into Apatite-forming Ability of Hydroxyapatite/Chitosan-based Scaffold

2019 ◽  
Vol 35 (3) ◽  
Author(s):  
Tran Thanh Hoai ◽  
Nguyen Kim Nga
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Inorganic Material ◽  
Porous Scaffolds ◽  
Mineral Layer ◽  
Chitosan Scaffold ◽  
Apatite Mineral ◽  
Highly Porous

In this study, porous scaffolds were fabricated using inorganic material-hydroxyapatite and chitosan for bone-tissue engineering. The combination of hydroxyapatite and chitosan may result in increasing biocompatibility of the scaffolds. The scaffolds were prepared by solvent casting and paticulate leaching method. Bioactivity of the scaffolds was evaluated through in vitro experiments by soaking scaffold samples in simulated body fluid (SBF). The scaffolds obtained were highly porous and interconnected with a mean pore size of around 200µm and porosity about 79 %. The apatite-mineral layer was produced on the HAp/chitosan after 10 days of soaking in SBF, however, it was not observed on the chitosan scaffold after 10 days soaking. The results revealed that the HAp/chitosan scaffold showed better bioactivity than the chitosan scaffold. Keywords Scaffold, Chitosan, Apatite, SBF. In this study, porous scaffolds were fabricated using inorganic material-hydroxyapatite and chitosan for bone-tissue engineering. The combination of hydroxyapatite and chitosan may result in increasing biocompatibility of the scaffolds. The scaffolds were prepared by solvent casting and paticulate leaching method. Bioactivity of the scaffolds was evaluated through in vitro experiments by soaking scaffold samples in simulated body fluid (SBF). The scaffolds obtained were highly porous and interconnected with a mean pore size of around 200µm and porosity about 79 %. The apatite-mineral layer was produced on the HAp/chitosan after 10 days of soaking in SBF, however, it was not observed on the chitosan scaffold after 10 days soaking. The results revealed that the HAp/chitosan scaffold showed better bioactivity than the chitosan scaffold. Keywords: Scaffold, Chitosan, Apatite, SBF.   In this study, porous scaffolds were fabricated using inorganic material-hydroxyapatite and chitosan for bone-tissue engineering. The combination of hydroxyapatite and chitosan may result in increasing biocompatibility of the scaffolds. The scaffolds were prepared by solvent casting and paticulate leaching method. Bioactivity of the scaffolds was evaluated through in vitro experiments by soaking scaffold samples in simulated body fluid (SBF). The scaffolds obtained were highly porous and interconnected with a mean pore size of around 200µm and porosity about 79 %. The apatite-mineral layer was produced on the HAp/chitosan after 10 days of soaking in SBF, however, it was not observed on the chitosan scaffold after 10 days soaking. The results revealed that the HAp/chitosan scaffold showed better bioactivity than the chitosan scaffold. Keywords: Scaffold, Chitosan, Apatite, SBF. References [1] M.P. Bostrom, D.A. Seigerman, The clinical use of allografts, demineralized bone matrices, synthetic bone graft substitutes and osteoinductive growth factors: a survey study, Hss. Journal 1 (2005) 9-18. https://doi.org/10. 1007/s11420-005-0111-5.[2] T.T. Hoai, N.K Nga, L.T. Giang, T.Q. Huy, P.N.M. Tuan, B.T.T. Binh, Hydrothermal Synthesis of Hydroxyapatite Nanorods for Rapid Formation of Bone-Like Mineralization, J. Electron. Mater. 46 (2017) 5064-5072. https:// doi.org/10.1007/s11664-017-5509-6.[3] M. Rinaudo, Chitin and chitosan: properties and applications, Prog. Polym. Sci. 31 (2006) 603-632. https://doi.org/10.1016/j.progpolymsci.2006. 06.001.[4] N.K. Nga, H.D. Chinh, P.T.T Hong, T.Q. Huy, Facile chitosan films for high performance removal of reactive blue 19 dye from aqueous solution, J. Polym. Environ. 25 (2007) 146-155. https://doi.org/10.1007/s10924-016-0792-5.[5] M.N.V Ravi Kumar, R.A.A Muzzarelli, H. Sashiwa, A.J. Domb, Chitosan chemistry and pharmaceutical perspectives, Chem. Rev. 104 (2004) 6017-6084. https://doi.org/10.1021/cr03 0441b.[6] J.M. Karp, M.S. Shoichet, J.E. Davies, Bone formation on two‐dimensional poly (DL‐lactide‐co‐glycolide)(PLGA) films and three‐dimensional PLGA tissue engineering scaffolds in vitro, J. Biomed. Mater. Res. A 64 (2003) 388-396. https://doi.org/10.1002/jbm.a.10420.[7] J.F. Mano, R.L. Reis, Osteochondral defects: present situation and tissue engineering approaches, J. Tissue. Eng. Regen. Med. 1 (2007) 261-273. https://doi.org/10.1002/term.37. [8] A.G. Mikos, J.S. Temenoff, Formation of highly porous biodegradable scaffolds for tissue engineering, Electron. J. Biotechn. 3 (2000) 23-24. http://dx.doi.org/10.4067/S0717-3458200000 0200003.[9] W.W. Thein-Han, R.D.K Misra, Biomimetic chitosan–nanohydroxyapatite composite scaffolds for bone tissue engineering, Acta Biomater. 5 (2009) 1182–1197. https://doi.org/ 10.1016/j.actbio.2008.11.025.[10] Y. Zhang, J.R. Venugopal, A.E. Turki, S. Ramakrishna, B. Su, C.T. Lim, Electrospun biomimetic nanocomposite nanofibers of hydroxyapatite/chitosan for bone tissue engineering, Biomaterials 29 (2008) 4314–4322. https://doi.org/10.1016/j.biomaterials.2008.07.038.[11] B.X. Vương, Tổng hợp và đặc trưng vật liệu composite hydroxyapatite/chitosan ứng dụng trong kỹ thuật y sinh.,Tạp chí Khoa học ĐHQGHN: Khoa học Tự nhiên và Công nghệ Tập 34 (2018) 9-15. https://doi.org/10.25073/ 2588-1140/vnunst.4689.[12] N.K. Nga, T.T. Hoai, P.H. Viet, Biomimetic scaffolds based on hydroxyapatite nanorod/poly (D, L) lactic acid with their corresponding apatite-forming capability and biocompatibility for bone-tissue engineering, Colloids Surf. B Biointerf. 128 (2015) 506-514. https://doi.org/10. 1016/j.colsurfb.2015.03.001.[13] N.K. Nga, L.T. Giang, T.Q. Huy, C. Migliaresi, Surfactant-assisted size control of hydroxyapatite nanorods for bone tissue engineering, Colloids Surf. B: Biointerf. 116 (2014) 666-673. https://doi.org/10.1016/j.colsurfb.2013.11.001.[14] C.R. Kothapalli, M.T. Shaw, M. Wei, Biodegradable HA-PLA 3-D porous scaffolds: effect of nano-sized filler content on scaffold properties, Acta Biomater. 1 (2005) 653-662. https://doi.org/10.1016/j.actbio.2005.06.005.[15] T. Kokubo, H. Takadama, How useful is SBF in predicting in vivo bone bioactivity?, Biomaterials 27 (2006) 2907-2915. https://doi.org/10.1016/j. biomaterials.2006.01.017[16] T.T. Hoai, N.K. Nga, Effect of pore architecture on osteoblast adhesion and proliferation on hydroxyapatite/poly (D, L) lactic acid-based bone scaffolds, J. Iran. Chem. Soc. 15 (2018) 1663-1671. https://doi.org/10.1007/s13738-018-1365-4.        

scholarly journals Up-Cycling of LCD Glass by Additive Manufacturing of Porous Translucent Glass Scaffolds

Materials ◽  
2021 ◽  
Vol 14 (17) ◽  
pp. 5083
Author(s):  
Arish Dasan ◽  
Paulina Ożóg ◽  
Jozef Kraxner ◽  
Hamada Elsayed ◽  
Elena Colusso ◽  
...  
Keyword(s):  
Additive Manufacturing ◽  
Viscous Flow ◽  
Porous Ceramic ◽  
Porous Scaffolds ◽  
Organic Additives ◽  
Burn Out ◽  
Ceramic Components ◽  
High Transition ◽  
Manufacturing Technologies ◽  
Highly Porous

Additive manufacturing technologies, compared to conventional shaping methods, offer great opportunities in design versatility, for the manufacturing of highly porous ceramic components. However, the application to glass powders, later subjected to viscous flow sintering, involves significant challenges, especially in shape retention and in the achievement of a substantial degree of translucency in the final products. The present paper disclosed the potential of glass recovered from liquid crystal displays (LCD) for the manufacturing of highly porous scaffolds by direct ink writing and masked stereolithography of fine powders mixed with suitable organic additives, and sintered at 950 °C, for 1–1.5 h, in air. The specific glass, featuring a relatively high transition temperature (Tg~700 °C), allowed for the complete burn-out of organics before viscous flow sintering could take place; in addition, translucency was favored by the successful removal of porosity in the struts and by the resistance of the used glass to crystallization.

scholarly journals Vascular Wall–Mesenchymal Stem Cells Differentiation on 3D Biodegradable Highly Porous CaSi-DCPD Doped Poly (α-hydroxy) Acids Scaffolds for Bone Regeneration

Nanomaterials ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 243 ◽  
Author(s):  
Monica Forni ◽  
Chiara Bernardini ◽  
Fausto Zamparini ◽  
Augusta Zannoni ◽  
Roberta Salaroli ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
In Vitro Model ◽  
Smooth Muscle Actin ◽  
Vascular Wall ◽  
Porous Scaffolds ◽  
Alpha Smooth Muscle Actin ◽  
Vitro Model ◽  
Highly Porous

Vascularization is a crucial factor when approaching any engineered tissue. Vascular wall–mesenchymal stem cells are an excellent in vitro model to study vascular remodeling due to their strong angiogenic attitude. This study aimed to demonstrate the angiogenic potential of experimental highly porous scaffolds based on polylactic acid (PLA) or poly-e-caprolactone (PCL) doped with calcium silicates (CaSi) and dicalcium phosphate dihydrate (DCPD), namely PLA-10CaSi-10DCPD and PCL-10CaSi-10DCPD, designed for the regeneration of bone defects. Vascular wall–mesenchymal stem cells (VW-MSCs) derived from pig thoracic aorta were seeded on the scaffolds and the expression of angiogenic markers, i.e. CD90 (mesenchymal stem/stromal cell surface marker), pericyte genes α-SMA (alpha smooth muscle actin), PDGFR-β (platelet-derived growth factor receptor-β), and NG2 (neuron-glial antigen 2) was evaluated. Pure PLA and pure PCL scaffolds and cell culture plastic were used as controls (3D in vitro model vs. 2D in vitro model). The results clearly demonstrated that the vascular wall mesenchymal cells colonized the scaffolds and were metabolically active. Cells, grown in these 3D systems, showed the typical gene expression profile they have in control 2D culture, although with some main quantitative differences. DNA staining and immunofluorescence assay for alpha-tubulin confirmed a cellular presence on both scaffolds. However, VW-MSCs cultured on PLA-10CaSi-10DCPD showed an individual cells growth, whilst on PCL-10CaSi-10DCPD scaffolds VW-MSCs grew in spherical clusters. In conclusion, vascular wall mesenchymal stem cells demonstrated the ability to colonize PLA and PCL scaffolds doped with CaSi-DCPD for new vessels formation and a potential for tissue regeneration.

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