scholarly journals Effects of Trehalose Polycation End-Group Functionalization on Plasmid DNA Uptake and Transfection

2012 ◽  
Vol 13 (8) ◽  
pp. 2229-2239 ◽  
Author(s):  
Kevin Anderson ◽  
Antons Sizovs ◽  
Mallory Cortez ◽  
Chris Waldron ◽  
D. M. Haddleton ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Dna Uptake ◽  
End Group Functionalization ◽  
End Group

scholarly journals End group functionalization of poly(ethylene glycol) with phenolphthalein: towards star-shaped polymers based on supramolecular interactions

2014 ◽  
Vol 10 ◽  
pp. 2263-2269 ◽  
Author(s):  
Carolin Fleischmann ◽  
Hendrik Wöhlk ◽  
Helmut Ritter
Keyword(s):  
Ethylene Glycol ◽  
Cycloaddition Reaction ◽  
Supramolecular Interactions ◽  
Poly Ethylene Glycol ◽  
Naked Eye ◽  
Vis Spectroscopy ◽  
End Group Functionalization ◽  
Interesting Approach ◽  
Poly Ethylene ◽  
End Group

The synthesis of a new phenolphthalein azide derivative, which can be easily utilized in polymer analogous reactions, is presented. The subsequent cycloaddition reaction with propargyl-functionalized methoxypoly(ethylene glycol) yielded polymers bearing phenolphthalein as the covalently attached end group. In presence of per-β-cyclodextrin-dipentaerythritol, the formation of stable inclusion complexes was observed, representing an interesting approach towards the formation of star shaped polymers. The decolorization of a basic polymer solution caused by the complexation was of great advantage since this behavior enabled following the complex formation by UV–vis spectroscopy and even the naked eye.

Genetic transformation of Rhodopseudomonas sphaeroides by plasmid DNA

1982 ◽  
Vol 152 (1) ◽  
pp. 89-97
Author(s):  
C S Fornari ◽  
S Kaplan
Keyword(s):  
Plasmid Dna ◽  
Recombinant Dna ◽  
Effective Means ◽  
Viable Cell ◽  
Broad Host Range ◽  
Dna Uptake ◽  
Rhodopseudomonas Sphaeroides ◽  
Open Circular ◽  
Polyethylene Glycol 6000

A broad-host-range cloning vector, pUI81, was constructed in vitro from plasmids RSF1010 and pSL25 (a pBR322 derivative) and used to assay for transformation in Rhodopseudomonas sphaeroides. Washing cells with 500 mM Tris was an effective means of inducing competence for DNA uptake. Transformation frequencies as high as 10(-5) (transformants per viable cell) have been achieved by incubating Tris-treated cells with plasmid DNA, 100 mM CaCl2, and 20% polyethylene glycol 6000. Maximum frequencies were obtained when recipient cells were spread onto selective media after a 6.5-h outgrowth period in antibiotic-free medium. The structure (open circular versus closed, covalent circular), size, and concentration of plasmid DNA all significantly affected the transformation frequency. Four different plasmids, all small and suitable as cloning vectors, have been introduced by transformation into several different R. sphaeroides strains. Recombinant DNA carried on small, nonconjugative plasmids with broad host ranges can now be directly transferred to R. sphaeroides by this method.

Involvement of Specific Mechanism in Plasmid DNA Uptake by Mouse Peritoneal Macrophages

1998 ◽  
Vol 245 (3) ◽  
pp. 729-733 ◽  
Author(s):  
Toshihide Takagi ◽  
Miwa Hashiguchi ◽  
Ram I. Mahato ◽  
Hideaki Tokuda ◽  
Yoshinobu Takakura ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Peritoneal Macrophages ◽  
Dna Uptake ◽  
Specific Mechanism ◽  
Mouse Peritoneal Macrophages

scholarly journals Sequence-specific DNA uptake in transformation of Neisseria gonorrhoeae

1982 ◽  
Vol 152 (3) ◽  
pp. 1071-1077
Author(s):  
J F Graves ◽  
G D Biswas ◽  
P F Sparling
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Dna Sequence ◽  
Plasmid Dna ◽  
Cryptic Plasmid ◽  
Dna Uptake ◽  
Restriction Fragments

Piliated, competent gonococci are known to preferentially take up homologous transforming DNA into the cell. We examined the mechanism for DNA uptake with pFA10, a hybrid 11.5-kilobase (kb) penicillin-resistant (Pcr) plasmid composed of heterologous DNA from a 7.2-kb Pcr plasmid and homologous DNA from a 4.2-kb gonococcal cryptic plasmid. The presence of the gonococcal cryptic plasmid DNA in the hybrid resulted in markedly increased transformation efficiencies in isogenic crosses as compared with the parent 7.2-kb Pcr plasmid. Uptake of 32P-end-labeled MspI or TaqI restriction fragments of the hybrid was limited to fragments entirely derived from the 4.2-kb gonococcal cryptic plasmid, indicating that DNA uptake was probably dependent on the presence of a specific DNA sequence. Since Haemophilus DNA did not inhibit transformation by the hybrid Pcr plasmid, the gonococcal DNA uptake sequence is different from the known sequence involved in homologous DNA uptake by Haemophilus spp.

Ultrasound and microbubble mediated plasmid DNA uptake: A fast, global and multi-mechanisms involved process

2018 ◽  
Vol 273 ◽  
pp. 40-50 ◽  
Author(s):  
Ning Rong ◽  
Hao Zhou ◽  
Ruming Liu ◽  
Yan Wang ◽  
Zhenzhen Fan
Keyword(s):  
Plasmid Dna ◽  
Dna Uptake

scholarly journals End Group-Functionalization and Synthesis of Block-Copolythiophenes by Modified Nickel Initiators

2011 ◽  
Vol 44 (15) ◽  
pp. 6017-6025 ◽  
Author(s):  
Alfons Smeets ◽  
Pieter Willot ◽  
Julien De Winter ◽  
Pascal Gerbaux ◽  
Thierry Verbiest ◽  
...  
Keyword(s):  
End Group Functionalization ◽  
End Group
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