Nanofibers from Blends of Polyvinyl Alcohol and Polyhydroxy Butyrate As Potential Scaffold Material for Tissue Engineering of Skin

2010 ◽  
Vol 11 (12) ◽  
pp. 3413-3421 ◽  
Author(s):  
Ashraf Sh. Asran ◽  
Khashayar. Razghandi ◽  
Neha Aggarwal ◽  
Goerg H. Michler ◽  
T. Groth
Keyword(s):  
Tissue Engineering ◽  
Polyvinyl Alcohol ◽  
Scaffold Material ◽  
Polyhydroxy Butyrate

280 MULTILINEAGE POTENTIAL OF PORCINE BONE MARROW AND ADIPOSE-DERIVED MESENCHYMAL STEM CELLS IN 3-D ALGINATE HYDROGELS

2009 ◽  
Vol 21 (1) ◽  
pp. 237 ◽  
Author(s):  
D. Kim ◽  
A. J. Maki ◽  
H.-J. Kong ◽  
E. Monaco ◽  
M. Bionaz ◽  
...  
Keyword(s):  
Gene Expression ◽  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipogenic Differentiation ◽  
Cell Types ◽  
Scaffold Material ◽  
Over Time

Adipose tissue presents an appealing alternative to bone marrow as a source of mesenchymal stem cells (MSC). However, in order to enhance cell proliferation and differentiation, 3-dimensional (3-D) culture may be required. A 3-D culture has benefits due to its more in vivo-like environment. Further, to form a functional tissue, a scaffold material is required to ensure proper shape and allow for efficient delivery of nutrients and growth factors. Alginate, a resorbable hydrogel, is a potential injectable scaffold for fat and bone tissue engineering due to its high biocompatibility, gelation with calcium and slow dissolution in a physiologic environment. In the present study, we examined the viability, gene expression and morphology of MSC, isolated from porcine adipose (ADSC) and bone marrow (BMSC), during osteogenic and adipogenic differentiation in a 3D alginate hydrogel environment for 0, 7 and 14 days (d). ADSC and BMSC were infused into alginate hydrogels, which polymerized upon the addition of Ca+2 ions. Both stem cell types were differentiated into osteoblasts using 0.1 μm dexamethasone, 10 mm beta glycerophosphate and 50 μm ascorbic acid, whereas adipocytes were differentiated using 10 μm insulin, 1 μm dexamethasone, and 0.5 mm IBMX. Osteogenic differentiation was confirmed using alkaline phosphatase, Von Kossa, and alizarin red S staining and adipogenic differentiation was confirmed using Oil Red O. Cell viability and proliferation was quantified using the MTT assay. Gene expression was measured using qPCR. The morphology of ADSC and BMSC differentiated toward osteogenic lineages changed with both cell types forming osteogenic nodules over time. The nodules formed by ADSC were larger in diameter than those formed by BMSC. Unlike the osteogenic cells that formed nodules, the ADSC and BMSC differentiated into adipogenic cells showed no significant changes in cell size or aggregation. Gene expression results indicated increased PPARG expression in BMSC with time whereas ADSC showed a peak of expression on day 7 and then decreased. ADSC showed increased (14-fold) PPRG expression when compared with BMSC. ADSC had 160-fold less expression of ALP than BMSC. BMSC showed a 16-fold higher expression level of BGLAP than ADSC. ADSC showed a 15.8% higher expression than BMSC for COL1a1. Both ADSC and BMSC showed similar trends SPARC expression, but BMSC had a 12-fold higher expression of SPP1 than ADSC. In summary, both types of mesenchymal stem cells successfully differentiated into both lineages and maintained viability in the hydrogel over time. In conclusion, alginate is a viable scaffold material for the differentiation of mesenchymal stem cells for tissue engineering applications. These results allow for future studies using the pig as an in vivo fat and bone tissue engineering model. This research was supported by the Illinois Regenerative Medicine Institute.

Remodeling and Compaction in Tissue Engineered Small Vessels

2009 ◽  
Author(s):  
Ana L. F. Soares ◽  
Maria Stekelenburg ◽  
Frank Baaijens
Keyword(s):  
Tissue Engineering ◽  
Environmental Factors ◽  
Small Diameter ◽  
Scaffold Material ◽  
Biodegradable Scaffold ◽  
Mechanical Integrity ◽  
Small Vessels ◽  
Biomechanical Stimuli

Small-diameter vessels tissue engineering (TE) seeks to provide viable replacements to the native ones. The approach involves seeding autologous cells onto a biodegradable scaffold material and delivering the suitable environmental factors (biochemical and biomechanical stimuli) during culture via a conditioning protocol. Still, these replacements appear to lack the native mechanical integrity. TE protocols should then be improved and optimized.

Combined Effects of Scaffold Material and Mechanical Stimulation on the Formation of Tissue Engineered Constructs Using Tendon and Ligament Progenitor Cells

2013 ◽  
Author(s):  
Andrew P. Breidenbach ◽  
Nathaniel A. Dyment ◽  
Yinhui Lu ◽  
Jason T. Shearn ◽  
David W. Rowe ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Progenitor Cells ◽  
Three Dimensional ◽  
Musculoskeletal Injuries ◽  
Combined Effects ◽  
Joint Movement ◽  
Scaffold Material ◽  
Ligament Injuries ◽  
Promising Alternative

Tendon and ligament injuries account for one-third of all musculoskeletal injuries [1]. Collagen fibrils in these mechanosensitive tissues transmit forces to mobilize and stabilize joint movement. Donor tissues used to repair these tissues often lack the mechanical properties of the tissue they are replacing. One promising alternative using tissue engineering combines stem/progenitor cells in three-dimensional tissue engineered constructs (TECs).

Enhancing mechanical and biodegradation properties of polyvinyl alcohol/silk fibroin nanofibers composite patches for Cardiac Tissue Engineering

2019 ◽  
Vol 255 ◽  
pp. 126510 ◽  
Author(s):  
Mostafa M. Sayed ◽  
Hamouda M. Mousa ◽  
M.R. El-Aassar ◽  
Nehal M. El-Deeb ◽  
Nouby M Ghazaly ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Polyvinyl Alcohol ◽  
Silk Fibroin ◽  
Cardiac Tissue ◽  
Cardiac Tissue Engineering ◽  
Silk Fibroin Nanofibers

scholarly journals Biomimetically Reinforced Polyvinyl Alcohol-Based Hybrid Scaffolds for Cartilage Tissue Engineering

Polymers ◽  
2017 ◽  
Vol 9 (12) ◽  
pp. 655 ◽  
Author(s):  
Hwan Kim ◽  
Yunsup Lee ◽  
Yunhye Kim ◽  
Yongsung Hwang ◽  
Nathaniel Hwang
Keyword(s):  
Tissue Engineering ◽  
Polyvinyl Alcohol ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Hybrid Scaffolds
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