Cross-Sectional Analysis of the Polysaccharide Composition in Cellulosic Fiber Materials by Enzymatic Peeling/High-Performance Capillary Zone Electrophoresis

2005 ◽  
Vol 6 (6) ◽  
pp. 3146-3151 ◽  
Author(s):  
John Sjöberg ◽  
Antje Potthast ◽  
Thomas Rosenau ◽  
Paul Kosma ◽  
Herbert Sixta
Keyword(s):  
Capillary Zone Electrophoresis ◽  
High Performance ◽  
Cross Sectional ◽  
Cross Sectional Analysis ◽  
Enzymatic Peeling ◽  
Zone Electrophoresis ◽  
Capillary Zone ◽  
Polysaccharide Composition ◽  
Fiber Materials ◽  
Sectional Analysis

scholarly journals High-performance capillary electrophoresis of core histones and their acetylated modified derivatives

1992 ◽  
Vol 283 (2) ◽  
pp. 467-471 ◽  
Author(s):  
H Lindner ◽  
W Helliger ◽  
A Dirschlmayer ◽  
M Jaquemar ◽  
B Puschendorf
Keyword(s):  
Capillary Electrophoresis ◽  
Capillary Zone Electrophoresis ◽  
Rat Liver ◽  
Phosphate Buffer ◽  
High Performance ◽  
Buffer System ◽  
Zone Electrophoresis ◽  
Core Histones ◽  
Capillary Zone ◽  
High Performance Capillary Electrophoresis

By using high-performance capillary electrophoresis, we have successfully separated rat liver core histones into several subfractions. Inconvenient interactions of the highly basic proteins with the capillary wall were eliminated by a phosphate buffer system containing 0.03% hydroxyprophylmethylcellulose. Sample amounts of a few nanolitres were analysed within about 20 min. Multiacetylated histones H4 and H3 from induced Friend erythroleukaemic cells prepurified by h.p.l.c. were clearly separated into their non-acetylated and distinct acetylated forms. Our results illustrate that the application of capillary zone electrophoresis on its own or in combination with h.p.l.c. to the analysis of histones provides an important new alternative to traditional gel electrophoreses.

Unexpected Discovery of Hemoglobin D Iran Families in Punjab, Pakistan by using two different Screening Methods

2021 ◽  
Vol 15 (11) ◽  
pp. 2893-2896
Author(s):  
Nada Sikander ◽  
Shabnam Bashir ◽  
Rija Tariq ◽  
Furqan Sabir ◽  
Samia Akhtar ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Cation Exchange ◽  
Capillary Zone Electrophoresis ◽  
High Performance ◽  
Screening Methods ◽  
Compound Heterozygous ◽  
Hb E ◽  
Zone Electrophoresis ◽  
Capillary Zone

Background: Hemoglobin D Iran is frequently misdiagnosed as Hb E or Hb D Punjab if only one method of screening is used. The objective of our study was to highlight the importance of using two different screening techniques in diagnosis of a hemoglobin variant, Hb D Iran in our case. Hematological parameters of heterozygous Hb D Iran and compound heterozygous β/Hb D Iran were also compared. Methods: A descriptive study was carried out on results of 52,379 subjects which were part of thalassemia extended family cascade screening from 36 districts of Punjab from October 2019-March 2021. Cases of Hb D Punjab and Hb E were run on both CE-HPLC (cation exchange-high performance liquid chromatography) and CZE (capillary zone electrophoresis). Resulting Hb D Iran cases were confirmed by ARMS-PCR (Amplification refractory mutation system-polymerase chain reaction). Results: Forty cases of Hb D Iran were detected out of 160 initially suspected Hb D Punjab cases and 126 Hb E cases. Diagnosis was confirmed by molecular analysis. Statistical significance was found between RBC count, MCV, MCH, Hb F and diagnosis of “heterozygous Hb D Iran” and “compound heterozygous for β/ Hb D Iran”. Conclusion: Hb D Iran can be easily missed and misdiagnosed as Hb E or Hb D Punjab, if two screening methods are not used. This maybe a reason why Hb D Iran remains unreported in our region. CBC and HPLC indices can also be suggestive if a case is of heterozygous D Iran or compound heterozygous β/Hb D Iran. Keywords: Hb D Iran, Hb E, Hb D Punjab, Cation exchange High performance liquid chromatography, Capillary zone electrophoresis

Comparison of High Resolution HPLC with Capillary Zone Electrophoresis in Hemoglobin A2 Measurement.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 3832-3832
Author(s):  
Monica V. Gallivan ◽  
Laura A. Worfolk ◽  
Christina D. Lapuz ◽  
Olivia Saqui ◽  
Larielyn P. Pan ◽  
...  
Keyword(s):  
High Resolution ◽  
Capillary Zone Electrophoresis ◽  
High Performance ◽  
Surrogate Marker ◽  
Beta Thalassemia ◽  
Structural Variants ◽  
Alpha Thalassemia ◽  
Zone Electrophoresis ◽  
Capillary Zone ◽  
Hemoglobin A2

Abstract With the advent of high performance liquid chromatography (HPLC) accurate and precise measurement of hemoglobin A2 has been available for over 20 years. When microcytosis is present an elevated A2 is used as a surrogate marker for identification of beta thalassemia carriers. This combination is a very useful screening test. In contrast, A2 measurement in the low range is used to a lesser extent in screening for alpha thalassemia, delta chain variants and delta thalassemia. In the absence of structural variants A2 measurement by HPLC is accurate in the normal, high, and low ranges. This accuracy however does not hold true when samples harbor structural variants, particularly in the presence of Hb S as there are glycated and degraded fractions of S that co-elute with A2, thus falsely elevating the A2 value. In addition, with E and “D” type variants there is no A2 result or a falsely decreased value because A2 partially or completely co-elutes with the variant. In contrast to HPLC, capillary zone electrophoresis (CZE) separates the hemoglobin fractions according to their electrophoretic mobility with an alkaline buffer. We compared HbA2 measurement by high resolution (HR) HPLC (Primus Diagnostics) and CZE (Sebia CapillaryS) in samples without structural variants in the normal, high, and low ranges and in samples containing common structural variants. The 112 normals were from healthy, hematologically normal adult volunteers. The 20 samples in the high A2 range did not include structural variants and all had microcytosis. The 42 low A2 samples were from individuals without microcytosis in whom isoelectricfocusing (IEF) was also performed to identify delta chain variants. The heterozygotes for S, C, E and “D” had adult phenotypes and based on the percentages of the variant did not appear to have alpha thalassemia. In the “D” group there were 6 D-Punjab, 1 Osu-Christiansburg, and 1 Korle-Bu. G-Philadelphia was excluded as it occurs in association with alpha thalassemia. The results of our comparison study are shown in Table I. Besides what is indicated in the table we identified 9 individuals in the low A2 group that had delta chain variants identified on CZE but silent on HR-HPLC. These delta variants were confirmed by IEF. In summary, in the absence of structural variants these data indicate excellent correlation between HR-HPLC and CZE in the normal, high and low A2 ranges. In the low A2 range CZE has an added advantage over HR-HPLC in detecting delta variants. In the C-Trait group both methodologies appear equivalent but as expected in the S-Trait, E-Trait, and “D” Trait groups the CZE value appears to be more accurate. Compared to the normal group, however, there is a slight positive bias in S-Trait and E-Trait. In conclusion both methodologies appear complementary and can be used in combination for greater accuracy in hemoglobin identification and quantification of fractions. Table I Parameter N HR-HPLC Mean HR-HPLC 1 SD CZE Mean CZE 1 SD y R value Normal range A2 (1.8–3.5) 112 2.6 0.17 2.6 0.15 1.02 0.90 High range A2 > 3.6 20 5.1 0.59 5.0 0.5 0.98 0.94 Low range A <1.8 42 1.4 0.25 1.5 0.29 1.08 0.91 S Trait 32 4.0 0.25 3.1 0.18 0.76 0.66 C trait 15 3.1 0.28 3.1 0.3 0.98 0.66 E Trait 12 2.0 0.55 3.4 0.41 1.52 0.38 “D” Trait 8 N/A N/A 2.9 0.18 N/A N/A

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