Photon Emission by Heme-Protein in the Presence of Reactive Oxygen Species and Phenolic Compounds

1998 ◽  
pp. 256-265 ◽  
Author(s):  
Y. Yoshiki ◽  
M. Kawane ◽  
T. Iida ◽  
H. Yuan ◽  
Okubo ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Phenolic Compounds ◽  
Photon Emission ◽  
Reactive Oxygen ◽  
Heme Protein ◽  
Oxygen Species

scholarly journals The Effects of Bioactive Compounds from Blueberry and Blackcurrant Powder on Oat Bran Pastes: Enhancing In Vitro Antioxidant Activity and Reducing Reactive Oxygen Species in Lipopolysaccharide-Stimulated Raw264.7 Macrophages

Antioxidants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 388
Author(s):  
Xiao Dan Hui ◽  
Gang Wu ◽  
Duo Han ◽  
Xi Gong ◽  
Xi Yang Wu ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Bioactive Compounds ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Raw264.7 Macrophages ◽  
Oat Bran

In this study, blueberry and blackcurrant powder were chosen as the phenolic-rich enrichments for oat bran. A Rapid Visco Analyser was used to form blueberry and blackcurrant enriched oat pastes. An in vitro digestion process evaluated the changes of phenolic compounds and the in vitro antioxidant potential of extracts of pastes. The anthocyanidin profiles in the extracts were characterised by the pH differential method. The results showed that blueberry and blackcurrant powder significantly increased the content of phenolic compounds and the in vitro antioxidant capacity of pastes, while the total flavonoid content decreased after digestion compared to the undigested samples. Strong correlations between these bioactive compounds and antioxidant values were observed. Lipopolysaccharide-stimulated RAW264.7 macrophages were used to investigate the intracellular antioxidant activity of the extracts from the digested oat bran paste with 25% enrichment of blueberry or blackcurrant powder. The results indicated that the extracts of digested pastes prevented the macrophages from experiencing lipopolysaccharide (LPS)-stimulated intracellular reactive oxygen species accumulation, mainly by the Kelch-like ECH-associated protein 1 (Keap1)/nuclear factor erythroid 2-related factor 2 (Nrf2) signalling pathway. These findings suggest that the bioactive ingredients from blueberry and blackcurrant powder enhanced the in vitro and intracellular antioxidant capacity of oat bran pastes, and these enriched pastes have the potential to be utilised in the development of the functional foods.

scholarly journals Aqueous Extract of Cocoa Phenolic Compounds Protects Differentiated Neuroblastoma SH-SY5Y Cells from Oxidative Stress

Biomolecules ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1266
Author(s):  
Noelia Carballeda Sangiao ◽  
Susana Chamorro ◽  
Sonia de Pascual-Teresa ◽  
Luis Goya
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Phenolic Compounds ◽  
Protective Effect ◽  
Aqueous Extract ◽  
Antioxidant Properties ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Human Neuroblastoma ◽  
Antioxidant Defense Enzymes

Cocoa is a rich source of polyphenols, especially flavanols and procyanidin oligomers, with antioxidant properties, providing protection against oxidation and nitration. Cocoa phenolic compounds are usually extracted with methanol/ethanol solvents in order to obtain most of their bioactive compounds; however, aqueous extraction seems more representative of the physiological conditions. In this study, an aqueous extract of cocoa powder has been prepared and chemically characterized, and its potential protective effect against chemically-induced oxidative stress has been tested in differentiated human neuroblastoma SH-SY5Y cells. Neuronal-like cultured cells were pretreated with realistic concentrations of cocoa extract and its major monomeric flavanol component, epicatechin, and then submitted to oxidative stress induced by a potent pro-oxidant. After one hour, production of reactive oxygen species was evaluated by two different methods, flow cytometry and in situ fluorescence by a microplate reader. Simultaneously, reduced glutathione and antioxidant defense enzymes glutathione peroxidase and glutathione reductase were determined and the results used for a comparative analysis of both ROS (reactive oxygen species) methods and to test the chemo-protective effect of the bioactive products on neuronal-like cells. The results of this approach, never tested before, validate both analysis of ROS and indicate that concentrations of an aqueous extract of cocoa phenolics and epicatechin within a physiological range confer a significant protection against oxidative insult to neuronal-like cells in culture.

scholarly journals Reactive Oxygen Species Scavenging Activity of Jixueteng Evaluated by Electron Spin Resonance (ESR) and Photon Emission

2014 ◽  
Vol 9 (12) ◽  
pp. 1934578X1400901 ◽  
Author(s):  
Toshizo Toyama ◽  
Satoko Wada-Takahashi ◽  
Maomi Takamichi ◽  
Kiyoko Watanabe ◽  
Ayaka Yoshida ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Electron Spin Resonance ◽  
Photon Emission ◽  
Herbal Medicines ◽  
Reactive Oxygen ◽  
Scavenging Activity ◽  
Oxygen Species ◽  
Ros Scavenging ◽  
Spin Resonance ◽  
Luminescence Measurement

Jixueteng, the dried stem of Spatholobus suberectus Dunn (Leguminosae), is a traditional Chinese herbal medicine that is commonly classified as a herb that promotes blood circulation and can be used to treat blood stasis. The aim of this study was to examine the reactive oxygen species (ROS) scavenging activity of Jixueteng and other herbal medicines. The ROS scavenging activities of the water extracts of Jixueteng, Cnidium officinale and Salvia miltiorrhiza were examined using an electron spin resonance (ESR) technique and faint luminescence measurement. The ESR signal intensities of the superoxide anion (O2•-) and hydroxyl radical (HO•) were reduced more by Jixueteng than the other herbal medicines we tested. High photon emission intensity to hydrogen peroxide (H2O2) and HO• was observed in Jixueteng using the XYZ chemiluminescence system that was used as faint luminescence measurement and analysis. The results of the present study revealed that the ROS scavenging activity of 8% Jixueteng was the strongest among the herbal medicines we tested. It has been reported that Jixueteng includes various polyphenols. In the ROS scavenging activity by Jixueteng, it is supposed that the antioxidant activity caused by these polyphenols would contribute greatly. In conclusion, a water extract component of Jixueteng had potent free radical scavenging activity and an antioxidative effect that inhibited the oxidative actions of O2•-, H2O2 and HO•. Therefore, Jixueteng represents a promising therapeutic drug for reactive oxygen-associated pathologies.

scholarly journals Warming Alters Plant Chemical and Nutrient Compositions by Affecting Metabolites in Cunninghamia lanceolata (Lamb.) Hook

Forests ◽  
2019 ◽  
Vol 10 (7) ◽  
pp. 553
Author(s):  
Qiufang Zhang ◽  
Zhijie Yang ◽  
Tingting Chen ◽  
Xiaoying Gong ◽  
Decheng Xiong ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Amino Acids ◽  
Heat Stress ◽  
Chemical Composition ◽  
Phenolic Compounds ◽  
Reactive Oxygen ◽  
Moisture Stress ◽  
Cunninghamia Lanceolata ◽  
Oxygen Species ◽  
Plant Chemical

Research Highlights: Warming alters the chemical composition of Cunninghamia lanceolata (Lamb.) Hook, resulting in increased production of macromolecular compounds that protect against heat stress. Background and Objectives: Low latitude forests are experiencing obvious climatic warming; however, the plant physiological responses to warming are not well understood. As warming induces moisture stress, we hypothesized that warming activates metabolites (i.e., lipids, phenolic compounds, amino acids) and causes damage to the leaves, exemplified by the increased concentrations of reactive oxygen species. Materials and Methods: We conducted a warming experiment in a C. lanceolata plantation. Plant physiological traits associated with nutrient status, reactive oxygen species, antioxidant enzymes species, and metabolites were measured. Results: Warming altered the chemical composition of C. lanceolata as it increased C:N ratios of leaves and roots. In particular, the concentrations of N and P in leaves and roots were significantly decreased under the warming condition, which might be related to the biomass production, namely, a dilution effect. Under the warming condition, most of the phospholipid compounds and proteins significantly increased. Leaf C, carbohydrates, amino acids, organic acids, flavonoids, and phenolic compounds were identified to have significantly lower concentrations under the warming treatment than those under the control treatment. These results suggested that moisture stress under the warming treatment may drive C deficiency and metabolic restriction in plants. Conclusions: Under the warming condition, C. lanceolata changed its energy utilization strategy and invested more resources to produce macromolecular compounds for protecting against heat stress. Warming in sub-tropical forests alters plant chemical properties, and thus may have an important consequence for nutrient cycling and soil C sequestration.

scholarly journals Generation of Reactive Oxygen Species and Photon Emission from a Browned Product

2002 ◽  
Vol 66 (8) ◽  
pp. 1641-1645 ◽  
Author(s):  
Tetsuo IIDA ◽  
Yumiko YOSHIKI ◽  
Shinich SOMEYA ◽  
Kazuyoshi OKUBO
Keyword(s):  
Reactive Oxygen Species ◽  
Photon Emission ◽  
Reactive Oxygen ◽  
Oxygen Species
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