Photoaffinity Labeling of Diphtheria Toxin Fragment A with 8-Azidoadenosyl Nicotinamide Adenine Dinucleotide†

Biochemistry ◽  
1999 ◽  
Vol 38 (42) ◽  
pp. 13877-13886 ◽  
Author(s):  
Rita Lodaya ◽  
Steven R. Blanke ◽  
R. John Collier ◽  
James T. Slama
Keyword(s):  
Nicotinamide Adenine Dinucleotide ◽  
Diphtheria Toxin ◽  
Photoaffinity Labeling ◽  
Nicotinamide Adenine

scholarly journals STUDIES ON THE MODE OF ACTION OF DIPHTHERIA TOXIN

1964 ◽  
Vol 120 (6) ◽  
pp. 1019-1039 ◽  
Author(s):  
R. J. Collier ◽  
A. M. Pappenheimer
Keyword(s):  
Amino Acids ◽  
Hela Cells ◽  
Nicotinamide Adenine Dinucleotide ◽  
Ribonucleic Acid ◽  
Mode Of Action ◽  
Diphtheria Toxin ◽  
Polypeptide Chain ◽  
Nicotinamide Adenine ◽  
Low Concentrations ◽  
Rabbit Reticulocytes

It has been demonstrated that low concentrations of highly purified diphtheria toxin specifically inhibit incorporation of labeled amino acids into polypeptides in extracts from HeLa cells and from rabbit reticulocytes. No inhibition of incorporation occurs in the absence of a specific cofactor. This cofactor has been identified as nicotinamide adenine dinucleotide (NAD). It has been shown that it is one of the steps involving transfer of amino acids from soluble ribonucleic acid to the growing polypeptide chain that is affected by the toxin in the presence of NAD.

Sign in / Sign up

Export Citation Format

Share Document