The Heme Component of the Neutrophil NADPH Oxidase Complex Is a Target for Aryliodonium Compounds†

Biochemistry ◽  
1999 ◽  
Vol 38 (12) ◽  
pp. 3694-3703 ◽  
Author(s):  
Jacques Doussiere ◽  
Jacques Gaillard ◽  
Pierre V. Vignais
Keyword(s):  
Nadph Oxidase ◽  
Nadph Oxidase Complex

scholarly journals Characterization of the NADPH Oxidase Complex in Human Myometrial and Leiomyoma Smooth Muscle Cells.

2009 ◽  
Vol 81 (Suppl_1) ◽  
pp. 295-295
Author(s):  
Fernando Mesquita ◽  
Erica Marsh ◽  
Mayandi Sivaguru ◽  
Romana Nowak
Keyword(s):  
Smooth Muscle ◽  
Nadph Oxidase ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Nadph Oxidase Complex

scholarly journals Role of l-amino acid oxidase isolated from Calloselasma rhodostoma venom on neutrophil NADPH oxidase complex activation

Toxicon ◽  
2018 ◽  
Vol 145 ◽  
pp. 48-55 ◽  
Author(s):  
Mauro Valentino Paloschi ◽  
Charles Nunes Boeno ◽  
Jéssica Amaral Lopes ◽  
André Eduardo dos Santos da Rosa ◽  
Weverson Luciano Pires ◽  
...  
Keyword(s):  
Amino Acid ◽  
Nadph Oxidase ◽  
Acid Oxidase ◽  
Amino Acid Oxidase ◽  
Nadph Oxidase Complex ◽  
Calloselasma Rhodostoma

Exaggerated vasomotor response to ANG II in rats with fetal programming of hypertension associated with exposure to a low-protein diet during gestation

2006 ◽  
Vol 291 (4) ◽  
pp. R1060-R1068 ◽  
Author(s):  
C. Yzydorczyk ◽  
F. Gobeil ◽  
G. Cambonie ◽  
I. Lahaie ◽  
N. L. O. Lê ◽  
...  
Keyword(s):  
Nadph Oxidase ◽  
Renin Angiotensin System ◽  
Underlying Mechanism ◽  
Maximal Response ◽  
Ang Ii ◽  
Vasomotor Response ◽  
Enhanced Chemiluminescence ◽  
Low Protein ◽  
Diphenylene Iodonium ◽  
Nadph Oxidase Complex

The renin-angiotensin system plays a key role in the initiation and maintenance of elevated blood pressure associated with altered intrauterine milieu. The current studies were undertaken to verify whether vascular response to ANG II is increased in adult offspring of low-protein fed dams (LP) compared with control (CTRL) and if so, to examine underlying mechanism(s). ANG II-induced contraction of carotid rings was increased in LP (Emax, the maximum asymptote of the curve, relative to maximal response to KCl 80 mM: 230 ± 3% LP vs. 201 ± 2% CTRL, P < 0.05). In both groups, contraction to ANG II was mediated solely by AT1R. Responses to thromboxane A2 analog U-46619 and to KCl 80 mM under step increases in tension were similar between groups. Endothelium depletion enhanced contraction to ANG II in both groups, more so in LP. Blockade of endothelin formation had no effect on response to ANG II, and ANG-(1–7) did not elicit vasomotor response in either group. Superoxide dismutase (SOD) analog Tempol normalized LP without modifying CTRL response to ANG II. Basal levels of superoxide (aortic segments, lucigenin-enhanced chemiluminescence and fluorescent dye hydroethidine) were higher in LP. ANG II further increased superoxide production in LP only, and this was inhibited by coincubation with diphenylene iodonium or apocynin (inhibitor of NADPH oxidase complex). AT1R expression in carotid arteries was increased in LP, whereas SOD expression was unchanged. In conclusion, vasoconstriction to ANG II is exaggerated in this model of developmental programming of hypertension, secondary to enhanced vascular production of superoxide anion by NADPH oxidase with concomitant increase of AT1R expression.

Anionic lipid-induced conformational changes in human phagocyte flavocytochrome b precede assembly and activation of the NADPH oxidase complex

2012 ◽  
Vol 521 (1-2) ◽  
pp. 24-31 ◽  
Author(s):  
Ross M. Taylor ◽  
Marcia H. Riesselman ◽  
Connie I. Lord ◽  
Jeannie M. Gripentrog ◽  
Algirdas J. Jesaitis
Keyword(s):  
Nadph Oxidase ◽  
Conformational Changes ◽  
Anionic Lipid ◽  
Nadph Oxidase Complex ◽  
Flavocytochrome B

scholarly journals Inhibition of neutrophil function following exposure to the Aspergillus fumigatus toxin fumagillin

2010 ◽  
Vol 59 (6) ◽  
pp. 625-633 ◽  
Author(s):  
John P. Fallon ◽  
Emer P. Reeves ◽  
Kevin Kavanagh
Keyword(s):  
Immune Response ◽  
Nadph Oxidase ◽  
Aspergillus Fumigatus ◽  
Proteolytic Enzymes ◽  
Yeast Cells ◽  
Fungal Colonization ◽  
Central Component ◽  
Myeloperoxidase Activity ◽  
Microbial Infection ◽  
Nadph Oxidase Complex

The filamentous fungus Aspergillus fumigatus produces a variety of enzymes and toxins that may facilitate fungal colonization of tissue and evasion of the host immune response. One such toxin, fumagillin, was investigated for its ability to inhibit the action of neutrophils, which are a central component of the innate immune response to microbial infection. Neutrophils exposed to 2 μg fumagillin ml−1 for 25 min showed a significantly reduced ability to kill yeast cells (P<0.02), to phagocytose conidia of A. fumigatus (P<0.023) and to consume oxygen (P<0.032). The ability of neutrophils to generate superoxide is dependent upon the action of a functional NADPH oxidase complex which is composed of cytosolic (p40phox, p47phox, p67phox, Rac2) and membrane (gp91phox) proteins. Exposure of neutrophils to fumagillin inhibited the formation of the NADPH oxidase complex by blocking the translocation of p47phox from the cytosolic to the membrane fraction (P=0.02). In addition to the production of superoxide, neutrophils also undergo degranulation, which leads to the release of proteolytic enzymes that contribute to the microbicidal activity of the cell. Fumagillin-treated neutrophils showed reduced degranulation as evidenced by lower myeloperoxidase activity (P<0.019). Fumagillin-treated cells demonstrated reduced levels of F-actin, thus indicating that retarding the formation of F-actin may contribute to the inhibition of the structural rearrangements required in the activated neutrophil. This work indicates that fumagillin may contribute to reducing the local immune response by altering the activity of neutrophils and thus facilitate the continued persistence and growth of A. fumigatus in the host.

Sign in / Sign up

Export Citation Format

Share Document