Fourier Transform Infrared Study of the Cation Radical of P680 in the Photosystem II Reaction Center:  Evidence for Charge Delocalization on the Chlorophyll Dimer†

Biochemistry ◽  
1998 ◽  
Vol 37 (39) ◽  
pp. 13614-13625 ◽  
Author(s):  
Takumi Noguchi ◽  
Tatsuya Tomo ◽  
Yorinao Inoue
Keyword(s):  
Fourier Transform ◽  
Photosystem Ii ◽  
Reaction Center ◽  
Cation Radical ◽  
Fourier Transform Infrared ◽  
Infrared Study ◽  
Charge Delocalization

scholarly journals A difference Fourier-transform infrared study of two redox-active tyrosine residues in photosystem II

1993 ◽  
Vol 90 (23) ◽  
pp. 11024-11028 ◽  
Author(s):  
G M MacDonald ◽  
K A Bixby ◽  
B A Barry
Keyword(s):  
Fourier Transform ◽  
Photosystem Ii ◽  
Reaction Center ◽  
Fourier Transform Infrared ◽  
Active Species ◽  
Structural Factors ◽  
Infrared Study ◽  
Tyrosine Residues ◽  
Redox Active ◽  
Difference Fourier

Photosystem II, the photosynthetic water-oxidizing complex, contains two redox-active tyrosine residues. Although current models suggest that these tyrosines are located in symmetric positions in the reaction center, there are functional differences between them. To elucidate those structural factors that give rise to this functional asymmetry, we have used difference Fourier-transform infrared spectroscopy to obtain the vibrational difference spectrum associated with the oxidation of each of these redox-active residues. Isotopic labeling was employed to definitively assign vibrational lines to the redox-active tyrosines. This work has shown that the vibrational spectra of the two redox-active species are significantly different from each other. This result suggests that the structure of the redox-active residue helps to determine its role in electron transfer in the reaction center.

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