scholarly journals Determination of the Three-Dimensional Structure of CC Chemokine Monocyte Chemoattractant Protein 3 by1H Two-Dimensional NMR Spectroscopy

Biochemistry ◽  
1997 ◽  
Vol 36 (34) ◽  
pp. 10600-10600
Author(s):  
S. Meunier ◽  
J.-M. Bernassau ◽  
J.-C. Guillemot ◽  
P. Ferrara ◽  
H. Darbon
Keyword(s):  
Nmr Spectroscopy ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Two Dimensional ◽  
Three Dimensional Structure ◽  
Cc Chemokine ◽  
Monocyte Chemoattractant ◽  
Monocyte Chemoattractant Protein

scholarly journals Macrocyclic Tetramers – Structural Investigation of Peptide-Peptoid Hybrids

2021 ◽  
Author(s):  
Claudine Herlan ◽  
Anna Sonnefeld ◽  
Thomas Gloge ◽  
Julian Brückel ◽  
Luisa Schlee ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Bioactive Compounds ◽  
Rational Design ◽  
Structural Investigation ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
X Ray ◽  
Three Dimensional Structure ◽  
X Ray Scattering

The determination of the three-dimensional structure is a key step for the identification of new targets as well as the rational design of bioactive compounds. Herein, we report the synthesis and the structural elucidation of novel tetrameric macrocycles. Measurements were taken in solid and solution states with the help of X-ray scattering and NMR spectroscopy. The investigations made will help to find diverse applications for this new, promising compound class.

Determination of the Three-Dimensional Structure of the Bifunctional .alpha.-Amylase/Trypsin Inhibitor from Ragi Seeds by NMR Spectroscopy

Biochemistry ◽  
1995 ◽  
Vol 34 (26) ◽  
pp. 8281-8293 ◽  
Author(s):  
Stefan Strobl ◽  
Peter Muehlhahn ◽  
Reimond Bernstein ◽  
Ronald Wiltscheck ◽  
Klaus Maskos ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Trypsin Inhibitor ◽  
Three Dimensional ◽  
Alpha Amylase ◽  
Dimensional Structure ◽  
Three Dimensional Structure

Three-Dimensional Structure of Cloned T3 Connector Protein at 1.6nm Resolution

1990 ◽  
Vol 48 (1) ◽  
pp. 282-283
Author(s):  
José L. Carrascosa ◽  
José M. Valpuesta ◽  
Hisao Fujisawa
Keyword(s):  
Dna Sequences ◽  
Expression Vector ◽  
Three Dimensional ◽  
Deae Cellulose ◽  
Dna Packaging ◽  
Dimensional Structure ◽  
Two Dimensional ◽  
Freezing And Thawing ◽  
Three Dimensional Structure ◽  
Dimensional Reconstruction

The head to tail connector of bacteriophages plays a fundamental role in the assembly of viral heads and DNA packaging. In spite of the absence of sequence homology, the structure of connectors from different viruses (T4, Ø29, T3, P22, etc) share common morphological features, that are most clearly revealed in their three-dimensional structure. We have studied the three-dimensional reconstruction of the connector protein from phage T3 (gp 8) from tilted view of two dimensional crystals obtained from this protein after cloning and purification.DNA sequences including gene 8 from phage T3 were cloned, into Bam Hl-Eco Rl sites down stream of lambda promotor PL, in the expression vector pNT45 under the control of cI857. E R204 (pNT89) cells were incubated at 42°C for 2h, harvested and resuspended in 20 mM Tris HC1 (pH 7.4), 7mM 2 mercaptoethanol, ImM EDTA. The cells were lysed by freezing and thawing in the presence of lysozyme (lmg/ml) and ligthly sonicated. The low speed supernatant was precipitated by ammonium sulfate (60% saturated) and dissolved in the original buffer to be subjected to gel nitration through Sepharose 6B, followed by phosphocellulose colum (Pll) and DEAE cellulose colum (DE52). Purified gp8 appeared at 0.3M NaCl and formed crystals when its concentration increased above 1.5 mg/ml.

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