Single-Tryptophan Mutants of Monomeric Tryptophan Repressor:  Optical Spectroscopy Reveals Nonnative Structure in a Model for an Early Folding Intermediate†

Biochemistry ◽  
1998 ◽  
Vol 37 (21) ◽  
pp. 7850-7858 ◽  
Author(s):  
Xiao Shao ◽  
C. Robert Matthews
Keyword(s):  
Optical Spectroscopy ◽  
Folding Intermediate ◽  
Tryptophan Repressor

Using optical spectroscopy for the study of paper degradation

2006 ◽  
Vol 49 (1) ◽  
pp. 25-35 ◽  
Author(s):  
Mauro Missori ◽  
Marcofabio Righini
Keyword(s):  
Optical Spectroscopy ◽  
Paper Degradation

Properties of Ultraviolet Exposed Camptothecin Studied by Using Optical Spectroscopy Methods

2016 ◽  
Vol 19 (4) ◽  
pp. 319-324 ◽  
Author(s):  
Blanka Ziomkowska ◽  
Tomasz Wybranowski ◽  
Michal Cyrankiewicz ◽  
Stefan Kruszewski
Keyword(s):  
Optical Spectroscopy

Intraspecific differentiation of sandflies specimens by optical spectroscopy and multivariate analysis

2021 ◽  
Author(s):  
Aline E. Casaril ◽  
Carlos G. Santos ◽  
Bruno S. Marangoni ◽  
Sandro M. Lima ◽  
Luis H.C. Andrade ◽  
...  
Keyword(s):  
Multivariate Analysis ◽  
Optical Spectroscopy ◽  
Intraspecific Differentiation

Growth of pentacene on α−Al2O3 (0001) studied by in situ optical spectroscopy

2017 ◽  
Vol 1 (4) ◽  
Author(s):  
Lei Zhang ◽  
X. Fu ◽  
M. Hohage ◽  
P. Zeppenfeld ◽  
L. D. Sun
Keyword(s):  
Optical Spectroscopy ◽  
Α Al2o3

Accurate determination of the onset wavelength (λonset) in optical spectroscopy

2021 ◽  
Vol 265 ◽  
pp. 107544
Author(s):  
Austin M. Wallace ◽  
Christine Curiac ◽  
Jared H. Delcamp ◽  
Ryan C. Fortenberry
Keyword(s):  
Optical Spectroscopy ◽  
Accurate Determination

scholarly journals Pharmacological inactivation of the prion protein by targeting a folding intermediate

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Giovanni Spagnolli ◽  
Tania Massignan ◽  
Andrea Astolfi ◽  
Silvia Biggi ◽  
Marta Rigoli ◽  
...  
Keyword(s):  
Prion Protein ◽  
Prion Diseases ◽  
Cellular Prion Protein ◽  
Surface Glycoprotein ◽  
Folding Intermediate ◽  
Biological Regulation ◽  
Cell Surface Glycoprotein ◽  
Folding Intermediates ◽  
Protein Levels ◽  
Small Molecule Ligands

AbstractRecent computational advancements in the simulation of biochemical processes allow investigating the mechanisms involved in protein regulation with realistic physics-based models, at an atomistic level of resolution. These techniques allowed us to design a drug discovery approach, named Pharmacological Protein Inactivation by Folding Intermediate Targeting (PPI-FIT), based on the rationale of negatively regulating protein levels by targeting folding intermediates. Here, PPI-FIT was tested for the first time on the cellular prion protein (PrP), a cell surface glycoprotein playing a key role in fatal and transmissible neurodegenerative pathologies known as prion diseases. We predicted the all-atom structure of an intermediate appearing along the folding pathway of PrP and identified four different small molecule ligands for this conformer, all capable of selectively lowering the load of the protein by promoting its degradation. Our data support the notion that the level of target proteins could be modulated by acting on their folding pathways, implying a previously unappreciated role for folding intermediates in the biological regulation of protein expression.

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