Kinetic Mechanism and Cofactor Content of Soybean Root Nodule Urate Oxidase†

Biochemistry ◽  
1997 ◽  
Vol 36 (16) ◽  
pp. 4731-4738 ◽  
Author(s):  
Kalju Kahn ◽  
Peter A. Tipton
Keyword(s):  
Root Nodule ◽  
Kinetic Mechanism ◽  
Urate Oxidase ◽  
Soybean Root

Studies on possible routes of ammonium assimilation in soybean root nodule bacteroids

1973 ◽  
Vol 19 (12) ◽  
pp. 1493-1499 ◽  
Author(s):  
Stanley D. Dunn ◽  
Robert V. Klucas
Keyword(s):  
Glutamine Synthetase ◽  
Nitrogen Assimilation ◽  
Reductive Amination ◽  
Root Nodule ◽  
Root Nodules ◽  
Ammonium Assimilation ◽  
Kinetic Properties ◽  
Transferase Activity ◽  
Soybean Root

Glutamine amide–2-oxoglutarate aminotransferase NAD+ oxidoreductase (GOGAT), glutamine synthetase (GS), glutamate dehydrogenase (GD), and alanine dehydrogenase (AD) were studied in soybean root nodules. GS, GOGAT, and AD were present in bacteroids at levels that could account for ammonium assimilation, but GD activity was quite low. The total activities of GS and GD were higher in the cytosol than in the bacteroids by factors of 20 and 7, respectively, whereas GOGAT was not detected in the cytosol. GS (transferase activity) was inhibited by alanine, CTP, glycine, and tryptophan at 5 mM but was relatively unaffected by asparagine, aspartic acid, CMP, glucosamine, and histidine at 5 mM. GOGAT activity was unaffected by ATP, ADP, 8-hydroxyquinoline, and 1,10-phenanthroline but was inhibited by EDTA, citrate, and parachloromercuribenzoate. GOGAT activity (reductive amination) was also inhibited 97% by preincubation with 10−4 M azaserine for 30 min but GD activity was inhibited only 13%. The apparent Km values for NH4+ by AD was 7.4 × 10−3 M and by GD was 7.3 × 10−2 M while for glutamine by GOGAT it was 9.3 × 10−5 M. Activities and kinetic properties for these enzymes may suggest potential routes of nitrogen assimilation in vivo.

scholarly journals Effect of Changes in Shoot Carbon-Exchange Rate on Soybean Root Nodule Activity

1982 ◽  
Vol 69 (2) ◽  
pp. 432-436 ◽  
Author(s):  
Larry E. Williams ◽  
Theodore M. Dejong ◽  
Donald A. Phillips
Keyword(s):  
Exchange Rate ◽  
Root Nodule ◽  
Carbon Exchange ◽  
Soybean Root

scholarly journals Soybean Root Nodule cDNA Encoding Glutathione Reductase

1994 ◽  
Vol 104 (3) ◽  
pp. 1081-1082 ◽  
Author(s):  
X. Tang ◽  
M. A. Webb
Keyword(s):  
Glutathione Reductase ◽  
Root Nodule ◽  
Soybean Root

Utilization of nitrate by bacteroids of Bradyrhizobium japonicum in the soybean root nodule

Planta ◽  
1988 ◽  
Vol 174 (1) ◽  
pp. 51-58 ◽  
Author(s):  
C. Giannakis ◽  
D. J. D. Nicholas ◽  
W. Wallace
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Root Nodule ◽  
Soybean Root

scholarly journals Cellular Location and Concentration of Leghaemoglobin in Soybean Root Nodules

1973 ◽  
Vol 26 (4) ◽  
pp. 741 ◽  
Author(s):  
FJ Bergersen ◽  
DJ Goodchild
Keyword(s):  
Root Nodule ◽  
Root Nodules ◽  
Fresh Tissue ◽  
Cellular Location ◽  
Soybean Root ◽  
Tissue Sections

The reaction of leghaemoglobin (Lb) with oxidized 3,3'-diaminobenzidine has been used to demonstrate the localization of this haemoprotein within the membrane envelopes surrounding the bacteroids in soybean root nodule cells. In fresh tissue sections mounted in a modified Honda medium, the reagent stained the envelope contents a brick-red colour.

scholarly journals Purification and Characterization of Soybean Root Nodule Ferric Leghemoglobin Reductase

1991 ◽  
Vol 96 (1) ◽  
pp. 32-37 ◽  
Author(s):  
Lin Ji ◽  
Stephen Wood ◽  
Manuel Becana ◽  
Robert V. Klucas
Keyword(s):  
Root Nodule ◽  
Purification And Characterization ◽  
Soybean Root
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