Determination of Affinities forlckSH2 Binding Peptides Using a Sensitive Fluorescence Assay:  Comparison between the pYEEIP and pYQPQP Consensus Sequences Reveals Context-Dependent Binding Specificity

Biochemistry ◽  
1996 ◽  
Vol 35 (51) ◽  
pp. 16746-16752 ◽  
Author(s):  
Ruby C. Cousins-Wasti ◽  
Richard H. Ingraham ◽  
Maurice M. Morelock ◽  
Christine A. Grygon
1970 ◽  
Vol 65 (1_Suppl) ◽  
pp. S104-S121 ◽  
Author(s):  
E. E. Baulieu ◽  
J. P. Raynaud ◽  
E. Milgrom

ABSTRACT A brief review of the characteristics of steroid binding proteins found in the plasma and in some target organs is presented, followed by some general remarks on binding »specificity« and binding parameters. Useful techniques for measuring binding parameters at equilibrium are reported, both those which keep the equilibrium intact and those which implicate its disruption. A concept is developed according to which the determination of a specific steroid binding protein is based on the »differential dissociation« of the several steroid binding complexes present in most biological mixtures. Methods which allow determination of the kinetic parameters of the binding systems are also presented. Various representations of the binding and therefore different modes of graphic representation and calculation are discussed, including the recent »proportion graph« method.


Blood ◽  
2009 ◽  
Vol 113 (3) ◽  
pp. 679-687 ◽  
Author(s):  
Dunja Ferring-Appel ◽  
Matthias W. Hentze ◽  
Bruno Galy

Abstract Mice with total and constitutive iron regulatory protein 2 (IRP2) deficiency exhibit microcytosis and altered body iron distribution with duodenal and hepatic iron loading and decreased iron levels in splenic macrophages. To explore cell-autonomous and systemic context-dependent functions of IRP2 and to assess the systemic consequences of local IRP2 deficiency, we applied Cre/Lox technology to specifically ablate IRP2 in enterocytes, hepatocytes, or macrophages, respectively. This study reveals that the hepatic and duodenal manifestations of systemic IRP2 deficiency are largely explained by cell-autonomous functions of IRP2. By contrast, IRP2-deficient macrophages from otherwise IRP2-sufficient mice do not display the abnormalities of macrophages from systemically IRP2-deficient animals, suggesting that these result from IRP2 disruption in other cell type(s). Mice with enterocyte-, hepatocyte-, or macrophage-specific IRP2 deficiency display normal red blood cell and plasma iron parameters, supporting the notion that the microcytosis in IRP2-deficient mice likely reflects an intrinsic defect in hematopoiesis. This work defines the respective roles of IRP2 in the determination of critical body iron parameters such as organ iron loading and erythropoiesis.


1992 ◽  
Vol 22 (6) ◽  
pp. 1405-1412 ◽  
Author(s):  
Ton N. M. Schumacher ◽  
Grada M. Van Bleek ◽  
Marie-ThéRèSe Heemels ◽  
Karl Deres ◽  
Ka Wan Li ◽  
...  

Peptides 1992 ◽  
1993 ◽  
pp. 67-69 ◽  
Author(s):  
Michal Lebl ◽  
Kit S. Lam ◽  
Petr Kocis ◽  
Viktor Krchňák ◽  
Marcel Patek ◽  
...  
Keyword(s):  

Author(s):  
Abdelouahad Bayar ◽  
Khalid Sami

To justify texts, Arabic calligraphers use to stretch some letters with small flowing curves; the kashida instead of inserting blanks among words. Of course, such stretchings are context dependent. An adequate tool to support such writing may be based on a continuous mathematical model. The model has to take into account the motion of the qalam. The characters may be represented as outlines. Among the curves composing the characters outlines, some intersections are to be determined dynamically. In the Naskh style, the qalam‘s head behaves as a rigid rectangle in motion with a constant inclination. To determine the curves delimiting the set of points to shade when writing, we have to find out a mathematical way to compare plane curves. Moreover, as the PostScript procedure to produce a dynamic character, should be repeated whenever the letter is to draw, the development of a font supporting a continuous stretching model, allowing stretchable letters with no overlapping outlines, without optimization would be of a high cost in CPU time. In this chapter, some stretching models are given and discussed. A method to compare curves is presented. It allows the determination of the character encoding with eventually overlapping outlines. Then a way to approximate the curves intersection coefficients is given. This is enough to remove overlapping outlines. Some evaluations in time processing to confirm the adopted optimization techniques are also exposed.


2019 ◽  
Vol 584 ◽  
pp. 113384 ◽  
Author(s):  
Michał Szkop ◽  
Kamil Brygoła ◽  
Magdalena Janczewska ◽  
Tomasz Ciach

1997 ◽  
Vol 43 (6) ◽  
pp. 996-1002 ◽  
Author(s):  
Manfred A Kessler ◽  
Andreas Meinitzer ◽  
Walter Petek ◽  
Otto S Wolfbeis

Abstract We report a new automated fluorescence assay for determination of albumin in urine. The dye Albumin Blue 580 specifically binds to albumin with exhibition of strong red fluorescence. The albumin concentration is calculated from emission intensity at 616 nm (excitation at 590 nm) and a calibration curve. Two Cobas Fara programs cover working ranges of 2–200 and 1–50 mg/L with detection limits of 1.4 and 0.4 mg/L, respectively. Within-run CVs (n = 10) ranged from 1.7% (189 mg/L) to 8.9% (7.2 mg/L) for 2–200 mg/L and from 2.9% (43.3 mg/L) to 5.7% (2.3 mg/L) for the 1–50 mg/L range. A test of urine samples (n = 100) submitted to routine analysis gave results that agreed well with those by the Behring nephelometric assay: AB 580 = 0.922 (± 0.010) BNA + 4.16 (± 0.78). No interference was detected from other urine components, including several proteins and 46 drugs. The high specificity and sensitivity make the method ideal for determination of microalbuminuria. In addition, the method is fast, inexpensive, and well-suited for clinical laboratory application and thus may be used instead of immunoassays.


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