scholarly journals Probing Soluble Guanylate Cyclase Activation by CO and YC-1 Using Resonance Raman Spectroscopy

Biochemistry ◽  
2010 ◽  
Vol 49 (18) ◽  
pp. 3815-3823 ◽  
Author(s):  
Mohammed Ibrahim ◽  
Emily R. Derbyshire ◽  
Michael A. Marletta ◽  
Thomas G. Spiro
Keyword(s):  
Raman Spectroscopy ◽  
Guanylate Cyclase ◽  
Soluble Guanylate Cyclase ◽  
Resonance Raman Spectroscopy ◽  
Resonance Raman ◽  
Cyclase Activation ◽  
Guanylate Cyclase Activation

Structural characterization of nitric oxide-bound soluble Guanylate Cyclase using resonance Raman spectroscopy

2013 ◽  
Vol 17 (03) ◽  
pp. 240-246
Author(s):  
Biswajit Pal ◽  
Katsuhiro Tanaka ◽  
Shigeo Takenaka ◽  
Tajith B. Shaik ◽  
Teizo Kitagawa
Keyword(s):  
Nitric Oxide ◽  
Raman Spectroscopy ◽  
Guanylate Cyclase ◽  
Protein Interactions ◽  
Bound States ◽  
Soluble Guanylate Cyclase ◽  
Resonance Raman Spectroscopy ◽  
Resonance Raman ◽  
Bending Modes

Mammalian soluble Guanylate Cyclase (sGC), working as a physiological NO receptor, is investigated using resonance Raman spectroscopy for NO bound states with different saturation levels in the presence and absence of effectors. The Fe–NO (νFe–NO) and N–O (νN-O) stretching bands appeared at 521 and 1681 cm-1, respectively, without effectors, but νN-O was split into 1681 and 1699 cm-1 in the presence of GTP and shifted to 1687 cm-1 in the presence of YC-1 or BAY 41-2272, while νFe-NO remained unaltered. The split two νN-O bands were independent of NO saturation levels. GTP or YC-1/BAY 41-2272 altered the vinyl and propionate bending modes from 423 to 399 cm-1 and 376 to 367 cm-1, respectively. Based on these observations, allosteric effects on NO …protein interactions are discussed.

Effects of GTP on Bound Nitric Oxide of Soluble Guanylate Cyclase Probed by Resonance Raman Spectroscopy†

Biochemistry ◽  
1997 ◽  
Vol 36 (33) ◽  
pp. 10155-10160 ◽  
Author(s):  
Takeshi Tomita ◽  
Takashi Ogura ◽  
Shingo Tsuyama ◽  
Yoshio Imai ◽  
Teizo Kitagawa
Keyword(s):  
Nitric Oxide ◽  
Raman Spectroscopy ◽  
Guanylate Cyclase ◽  
Soluble Guanylate Cyclase ◽  
Resonance Raman Spectroscopy ◽  
Resonance Raman

scholarly journals Molecular steps in soluble guanylate cyclase activation

2005 ◽  
Vol 5 (Suppl 1) ◽  
pp. S1
Author(s):  
Elizabeth M Boon ◽  
Stephen PL Cary ◽  
Shirley H Huang ◽  
Jonathan A Winger ◽  
Michael A Marletta
Keyword(s):  
Guanylate Cyclase ◽  
Soluble Guanylate Cyclase ◽  
Cyclase Activation ◽  
Guanylate Cyclase Activation

Hydrogen peroxide elicits pulmonary arterial relaxation and guanylate cyclase activation

1987 ◽  
Vol 252 (4) ◽  
pp. H721-H732 ◽  
Author(s):  
T. M. Burke ◽  
M. S. Wolin
Keyword(s):  
Hydrogen Peroxide ◽  
Guanylate Cyclase ◽  
Superoxide Anion ◽  
Vascular Tone ◽  
Soluble Guanylate Cyclase ◽  
Compound I ◽  
Cyclase Activation ◽  
Arterial Relaxation ◽  
Pulmonary Arterial ◽  
Guanylate Cyclase Activation

Hydrogen peroxide produces concentration-dependent relaxation of precontracted isolated bovine intrapulmonary arterial rings by a mechanism which is independent of the endothelium or prostaglandin mediators. Relaxant responses to hydrogen peroxide concentrations of up to 100 microM were markedly attenuated by the inhibitor of soluble guanylate cyclase activation, methylene blue (10 microM). Micromolar concentrations of hydrogen peroxide elicit time- and concentration-dependent increase in arterial levels of guanosine 3',5'-cyclic monophosphate that are associated with decreases in force. Soluble guanylate cyclase activity is markedly activated by enzymatically generated hydrogen peroxide in a manner that is most closely associated with the concentration of catalase present in the assay, by a mechanism that is inhibited by superoxide anion and the inactivation of catalase. Our data are most consistent with the involvement of compound I, a species of catalase formed during the metabolism of peroxide, in the mechanism of guanylate cyclase activation. The nature of this mechanism of arterial relaxation suggests that it could contribute to the regulation of pulmonary vascular tone by oxygen tension.

Effects of soluble guanylate cyclase activation on heart transplantation in a rat model

2015 ◽  
Vol 34 (10) ◽  
pp. 1346-1353 ◽  
Author(s):  
Sivakkanan Loganathan ◽  
Sevil Korkmaz-Icöz ◽  
Tamás Radovits ◽  
Shiliang Li ◽  
Beatrice Mikles ◽  
...  
Keyword(s):  
Heart Transplantation ◽  
Guanylate Cyclase ◽  
Rat Model ◽  
Soluble Guanylate Cyclase ◽  
Cyclase Activation ◽  
Guanylate Cyclase Activation

Identification of residues crucially involved in soluble guanylate cyclase activation

FEBS Letters ◽  
2006 ◽  
Vol 580 (17) ◽  
pp. 4205-4213 ◽  
Author(s):  
Christiane Rothkegel ◽  
Peter M. Schmidt ◽  
Friederike Stoll ◽  
Henning Schröder ◽  
Harald H.H.W. Schmidt ◽  
...  
Keyword(s):  
Guanylate Cyclase ◽  
Soluble Guanylate Cyclase ◽  
Cyclase Activation ◽  
Guanylate Cyclase Activation
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