Use of Glycerol-Containing Media To Study the Intrinsic Fluorescence Properties of Detergent-Solubilized Native or Expressed SERCA1a†

Biochemistry ◽  
2008 ◽  
Vol 47 (46) ◽  
pp. 12159-12174 ◽  
Author(s):  
Cédric Montigny ◽  
Bertrand Arnou ◽  
Estelle Marchal ◽  
Philippe Champeil
2013 ◽  
Vol 15 (21) ◽  
pp. 8162 ◽  
Author(s):  
Jean-François Greisch ◽  
Michael E. Harding ◽  
Mattias Kordel ◽  
Wim Klopper ◽  
Manfred M. Kappes ◽  
...  

1996 ◽  
Vol 271 (44) ◽  
pp. 27249-27258 ◽  
Author(s):  
Paolo Neyroz ◽  
Carolina Menna ◽  
Eugenia Polverini ◽  
Lanfranco Masotti

2020 ◽  
Vol 18 (42) ◽  
pp. 8668-8676
Author(s):  
Constance M. Korkor ◽  
Larnelle F. Garnie ◽  
Leah Amod ◽  
Timothy J. Egan ◽  
Kelly Chibale

Intrinsic fluorescence properties, docking and a hemozoin inhibition assay were employed to study the mechanism of action of two structurally related pyrido-[1,2-a]benzimidazole derivatives.


2014 ◽  
Vol 43 (2) ◽  
pp. 246-248 ◽  
Author(s):  
Lei Cao ◽  
Dandan Jia ◽  
Shaofei Wang ◽  
Yunlong Rong ◽  
Chun Liu ◽  
...  

2006 ◽  
Vol 61 (9-10) ◽  
pp. 741-748 ◽  
Author(s):  
Michael U. Kumke ◽  
Carsten Dosche ◽  
Roman Flehr ◽  
Wolfram Trowitzsch-Kienast ◽  
Hans-Gerd Löhmannsröben

AbstractSiderophores play a very important role in the uptake process of iron by bacteria. Due to the so-called active transport the uptake of siderophores by bacteria is very specific, which makes the use of siderophores as effective shuttles for antibiotics in the treatment of infections and other diseases caused by bacteria highly attractive. In order to further investigate the transport and incorporation of siderophores into the bacteria cells, distinct molecular probes are needed. Especially artificial siderophores, that show a specific intrinsic fluorescence, are highly attractive for such monitoring purposes. A promising candidate of such a fluorescent artificial siderophore is bis-2,3-dihydroxybenzoyl-2,6-dimethylamino-pyridine (pyridinochelin, PY). The fluorescence properties of PY were investigated in different solvents and in the presence of different metal ions. It was found that PY in its free form shows a complex fluorescence behavior. In methanol a clear dual fluorescence is observed. In aqueous solution intermolecular interactions with water molecules are determining the intrinsic fluorescence. Upon complexation with metal ions (Me3+ = Eu3+, Tb3+, Al3+, Fe3+) the fluorescence characteristics changed. The fluorescence quantum yield of PY decreased upon addition of Me3+ - except for Al3+, which showed no fluorescence quenching. The fluorescence decay of PY loaded with metal ions showed a nicely mono-exponential fluorescence decay, which was in contrast to PY in the absence of metal ions. This drastic change in the fluorescence properties of PY upon metal ion complexation makes PY highly attractive as a fluorescence probe for the investigation of siderophore action and siderophore-mediated transport processes


1967 ◽  
Vol 64 ◽  
pp. 173-182 ◽  
Author(s):  
Erhard J. Schimitschek ◽  
Richard B. Nehrich Jr ◽  
John A. Trias

2000 ◽  
Vol 72 (3) ◽  
pp. 415 ◽  
Author(s):  
Joydip Das ◽  
Rosalie K. Crouch ◽  
Parkson Lee-Gau Chong

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