scholarly journals Mechanistic Investigations of the Dehydration Reaction of Lacticin 481 Synthetase Using Site-Directed Mutagenesis†

Biochemistry ◽  
2007 ◽  
Vol 46 (20) ◽  
pp. 5991-6000 ◽  
Author(s):  
Young Ok You ◽  
Wilfred A. van der Donk
Keyword(s):  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Dehydration Reaction ◽  
Lacticin 481 ◽  
Lacticin 481 Synthetase ◽  
Mechanistic Investigations

Mechanistic Investigations on Microbial Type I Terpene Synthases through Site-directed Mutagenesis

Synthesis ◽  
2021 ◽  
Author(s):  
Houchao Xu ◽  
Jeroen Dickschat
Keyword(s):  
Crystal Structures ◽  
Site Directed Mutagenesis ◽  
Terpene Synthase ◽  
Directed Mutagenesis ◽  
Type I ◽  
Terpene Synthases ◽  
Conserved Motifs ◽  
Sequence Identity ◽  
The Past ◽  
Mechanistic Investigations

During the past three decades many terpene synthases have been characterised from all kingdoms of life. The type I of these enzymes from bacteria, fungi and protists commonly exhibit several highly conserved motifs and single residues, and the available crystal structures show a shared -helical fold, while the overall sequence identity is generally low. Several enzymes have been studied by site-directed mutagenesis, giving valuable insights into terpene synthase catalysis and the intriguing mechanisms of terpene synthases. Some mutants are also preparatively useful and give higher yields than the wildtype or a different product that is otherwise difficult to access. The accumulated knowledge obtained from these studies is presented and discussed in this review.

The site-directed mutagenesis and prokaryotic expression of midkine gene in Cynoglossus semilaevis

2013 ◽  
Vol 37 (3) ◽  
pp. 330
Author(s):  
Yanan WANG ◽  
Xudong LIU ◽  
Linlin MU ◽  
Zhipeng LIU ◽  
Chunmei LI ◽  
...  
Keyword(s):  
Prokaryotic Expression ◽  
Cynoglossus Semilaevis ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis

Site-directed mutagenesis on alkaline phosphatasefrompseudomonas aeruginosapao1 for enhanced activity

2016 ◽  
Vol 7 (4) ◽  
Author(s):  
UMA SELVARAJ ◽  
THIRUMALAI MUTHUKUMARESAN ◽  
GAYATHRI VIJAYENDRAN ◽  
SENTHIL KUMAR DEVAN ◽  
VENU BABU P ◽  
...  
Keyword(s):  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Enhanced Activity

scholarly journals Activation of anthrachamycin biosynthesis in Streptomyces chattanoogensis L10 by site-directed mutagenesis of rpoB

2019 ◽  
Vol 20 (12) ◽  
pp. 983-994
Author(s):  
Zi-yue Li ◽  
Qing-ting Bu ◽  
Jue Wang ◽  
Yu Liu ◽  
Xin-ai Chen ◽  
...  
Keyword(s):  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Streptomyces Chattanoogensis
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