scholarly journals Intrapolypeptide Interactions between the GTPase Effector Domain (GED) and the GTPase Domain Form the Bundle Signaling Element in Dynamin Dimers

Biochemistry ◽  
2014 ◽  
Vol 53 (36) ◽  
pp. 5724-5726 ◽  
Author(s):  
Saipraveen Srinivasan ◽  
Juha-Pekka Mattila ◽  
Sandra L. Schmid
Keyword(s):  
Gtpase Domain ◽  
Effector Domain ◽  
Gtpase Effector Domain

scholarly journals NMR Derived Model of GTPase Effector Domain (GED) Self Association: Relevance to Dynamin Assembly

PLoS ONE ◽  
2012 ◽  
Vol 7 (1) ◽  
pp. e30109
Author(s):  
Swagata Chakraborty ◽  
Supriya Pratihar ◽  
Ramakrishna V. Hosur
Keyword(s):  
Effector Domain ◽  
Gtpase Effector Domain ◽  
Self Association

scholarly journals De novo missense variant in the GTPase effector domain (GED) of DNM1L leads to static encephalopathy and seizures

2019 ◽  
Vol 5 (3) ◽  
pp. a003673 ◽  
Author(s):  
Nurit Assia Batzir ◽  
Pranjali K. Bhagwat ◽  
Tanya N. Eble ◽  
Pengfei Liu ◽  
Christine M. Eng ◽  
...  
Keyword(s):  
De Novo ◽  
Missense Variant ◽  
Effector Domain ◽  
Gtpase Effector Domain ◽  
Static Encephalopathy

1H, 15N, 13C resonance assignment of 9.7 M urea-denatured state of the GTPase effector domain (GED) of dynamin

2008 ◽  
Vol 3 (1) ◽  
pp. 13-16 ◽  
Author(s):  
Jeetender Chugh ◽  
Shilpy Sharma ◽  
Dinesh Kumar ◽  
Ramakrishna V. Hosur
Keyword(s):  
Resonance Assignment ◽  
Effector Domain ◽  
Denatured State ◽  
Gtpase Effector Domain

scholarly journals Adult-onset autosomal dominant spastic paraplegia linked to a GTPase-effector domain mutation of dynamin 2

BMC Neurology ◽  
2015 ◽  
Vol 15 (1) ◽  
Author(s):  
Nyamkhishig Sambuughin ◽  
Lev G. Goldfarb ◽  
Tatiana M. Sivtseva ◽  
Tatiana K. Davydova ◽  
Vsevolod A. Vladimirtsev ◽  
...  
Keyword(s):  
Autosomal Dominant ◽  
Adult Onset ◽  
Spastic Paraplegia ◽  
Effector Domain ◽  
Gtpase Effector Domain ◽  
Dynamin 2

scholarly journals The GTPase Effector Domain Sequence of the Dnm1p GTPase Regulates Self-Assembly and Controls a Rate-limiting Step in Mitochondrial Fission

2001 ◽  
Vol 12 (9) ◽  
pp. 2756-2766 ◽  
Author(s):  
Noelle H. Fukushima ◽  
Ellen Brisch ◽  
Brian R. Keegan ◽  
William Bleazard ◽  
Janet M. Shaw
Keyword(s):  
Mitochondrial Fission ◽  
Yeast Two Hybrid ◽  
Effector Domain ◽  
Rate Limiting Step ◽  
Limiting Step ◽  
Gtpase Effector Domain ◽  
Rate Limiting ◽  
Two Hybrid

Dnm1p belongs to a family of dynamin-related GTPases required to remodel different cellular membranes. In budding yeast, Dnm1p-containing complexes assemble on the cytoplasmic surface of the outer mitochondrial membrane at sites where mitochondrial tubules divide. Our previous genetic studies suggested that Dnm1p's GTPase activity was required for mitochondrial fission and that Dnm1p interacted with itself. In this study, we show that bacterially expressed Dnm1p can bind and hydrolyze GTP in vitro. Coimmunoprecipitation studies and yeast two-hybrid analysis suggest that Dnm1p oligomerizes in vivo. With the use of the yeast two-hybrid system, we show that this Dnm1p oligomerization is mediated, in part, by a C-terminal sequence related to the GTPase effector domain (GED) in dynamin. The Dnm1p interactions characterized here are similar to those reported for dynamin and dynamin-related proteins that form higher order structures in vivo, suggesting that Dnm1p assembles to form rings or collars that surround mitochondrial tubules. Based on previous findings, a K705A mutation in the Dnm1p GED is predicted to interfere with GTP hydrolysis, stabilize active Dnm1p-GTP, and stimulate a rate-limiting step in fission. Here we show that expression of the Dnm1 K705A protein in yeast enhances mitochondrial fission. Our results provide evidence that the GED region of a dynamin-related protein modulates a rate-limiting step in membrane fission.

scholarly journals Intra- and Intermolecular Domain Interactions of the C-terminal GTPase Effector Domain of the Multimeric Dynamin-like GTPase Drp1

2004 ◽  
Vol 279 (34) ◽  
pp. 35967-35974 ◽  
Author(s):  
Peng-Peng Zhu ◽  
Andrew Patterson ◽  
Julia Stadler ◽  
Daniel P. Seeburg ◽  
Morgan Sheng ◽  
...  
Keyword(s):  
Effector Domain ◽  
Domain Interactions ◽  
Gtpase Effector Domain

scholarly journals Structural characterization of the large soluble oligomers of the GTPase effector domain of dynamin

FEBS Journal ◽  
2006 ◽  
Vol 273 (2) ◽  
pp. 388-397 ◽  
Author(s):  
Jeetender Chugh ◽  
Amarnath Chatterjee ◽  
Ashutosh Kumar ◽  
Ram Kumar Mishra ◽  
Rohit Mittal ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Effector Domain ◽  
Gtpase Effector Domain
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