Expression, Purification, and Reconstitution of a Diatom Silicon Transporter

Biochemistry ◽  
2012 ◽  
Vol 51 (18) ◽  
pp. 3776-3785 ◽  
Author(s):  
Paul Curnow ◽  
Laura Senior ◽  
Michael J. Knight ◽  
Kimberlee Thamatrakoln ◽  
Mark Hildebrand ◽  
...  
Keyword(s):  
Silicon Transporter

A pericycle‐localized silicon transporter for efficient xylem loading in rice

2022 ◽  
Author(s):  
Sheng Huang ◽  
Naoki Yamaji ◽  
Gen Sakurai ◽  
Namiki Mitani‐Ueno ◽  
Noriyuki Konishi ◽  
...  
Keyword(s):  
Silicon Transporter ◽  
Xylem Loading

scholarly journals A family of silicon transporter structural genes in a pennate diatom Synedra ulna subsp. danica (Kütz.) Skabitsch

PLoS ONE ◽  
2018 ◽  
Vol 13 (8) ◽  
pp. e0203161 ◽  
Author(s):  
Artyom M. Marchenkov ◽  
Darya P. Petrova ◽  
Alexey A. Morozov ◽  
Yulia R. Zakharova ◽  
Michael A. Grachev ◽  
...  
Keyword(s):  
Structural Genes ◽  
Pennate Diatom ◽  
Silicon Transporter

Isolation and functional characterization ofCsLsi1, a silicon transporter gene inCucumis sativus

2016 ◽  
Vol 159 (2) ◽  
pp. 201-214 ◽  
Author(s):  
Hao Sun ◽  
Jia Guo ◽  
Yaoke Duan ◽  
Tiantian Zhang ◽  
Heqiang Huo ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Transporter Gene ◽  
Silicon Transporter

scholarly journals Identification of a mammalian silicon transporter

2017 ◽  
Vol 312 (5) ◽  
pp. C550-C561 ◽  
Author(s):  
Sarah Ratcliffe ◽  
Ravin Jugdaohsingh ◽  
Julien Vivancos ◽  
Alan Marron ◽  
Rupesh Deshmukh ◽  
...  
Keyword(s):  
Higher Plants ◽  
Bone Development ◽  
Rat Kidney ◽  
Expression System ◽  
Reverse Direction ◽  
Xenopus Laevis Oocytes ◽  
Renal Epithelium ◽  
Silicon Transporter ◽  
Normal Rat ◽  
Dietary Phosphate

Silicon (Si) has long been known to play a major physiological and structural role in certain organisms, including diatoms, sponges, and many higher plants, leading to the recent identification of multiple proteins responsible for Si transport in a range of algal and plant species. In mammals, despite several convincing studies suggesting that silicon is an important factor in bone development and connective tissue health, there is a critical lack of understanding about the biochemical pathways that enable Si homeostasis. Here we report the identification of a mammalian efflux Si transporter, namely Slc34a2 (also termed NaPiIIb), a known sodium-phosphate cotransporter, which was upregulated in rat kidney following chronic dietary Si deprivation. Normal rat renal epithelium demonstrated punctate expression of Slc34a2, and when the protein was heterologously expressed in Xenopus laevis oocytes, Si efflux activity (i.e., movement of Si out of cells) was induced and was quantitatively similar to that induced by the known plant Si transporter OsLsi2 in the same expression system. Interestingly, Si efflux appeared saturable over time, but it did not vary as a function of extracellular [Formula: see text] or Na+ concentration, suggesting that Slc34a2 harbors a functionally independent transport site for Si operating in the reverse direction to the site for phosphate. Indeed, in rats with dietary Si depletion-induced upregulation of transporter expression, there was increased urinary phosphate excretion. This is the first evidence of an active Si transport protein in mammals and points towards an important role for Si in vertebrates and explains interactions between dietary phosphate and silicon.

scholarly journals Characterization of the Silicon Uptake System and Molecular Mapping of the Silicon Transporter Gene in Rice

2004 ◽  
Vol 136 (2) ◽  
pp. 3284-3289 ◽  
Author(s):  
Jian Feng Ma ◽  
Namiki Mitani ◽  
Sakiko Nagao ◽  
Saeko Konishi ◽  
Kazunori Tamai ◽  
...  
Keyword(s):  
Molecular Mapping ◽  
Uptake System ◽  
Transporter Gene ◽  
Silicon Transporter ◽  
Silicon Uptake

scholarly journals Screening and Expression of a Silicon Transporter Gene(Lsi1)in Wild-Type Indica Rice Cultivars

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
Mahbod Sahebi ◽  
Mohamed M. Hanafi ◽  
M. Y. Rafii ◽  
Parisa Azizi ◽  
Rambod Abiri ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Indica Rice ◽  
Rice Variety ◽  
Morphological Properties ◽  
Japonica Rice ◽  
Wild Type ◽  
Rice Varieties ◽  
Silicon Transporter ◽  
Chlorophyll A And B ◽  
Japonica Rice Variety

Silicon (Si) is one of the most prevalent elements in the soil. It is beneficial for plant growth and development, and it contributes to plant defense against different stresses. TheLsi1gene encodes a Si transporter that was identified in a mutant Japonica rice variety. This gene was not identified in fourteen Malaysian rice varieties during screening. Then, a mutant version ofLsi1was substituted for the native version in the three most common Malaysian rice varieties, MR219, MR220, and MR276, to evaluate the function of the transgene. Real-time PCR was used to explore the differential expression ofLsi1in the three transgenic rice varieties. Silicon concentrations in the roots and leaves of transgenic plants were significantly higher than in wild-type plants. Transgenic varieties showed significant increases in the activities of the enzymes SOD, POD, APX, and CAT; photosynthesis; and chlorophyll content; however, the highest chlorophyll A and B levels were observed in transgenic MR276. Transgenic varieties have shown a stronger root and leaf structure, as well as hairier roots, compared to the wild-type plants. This suggests thatLsi1plays a key role in rice, increasing the absorption and accumulation of Si, then alters antioxidant activities, and improves morphological properties.

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