scholarly journals Characterization of the Silicon Uptake System and Molecular Mapping of the Silicon Transporter Gene in Rice

2004 ◽  
Vol 136 (2) ◽  
pp. 3284-3289 ◽  
Author(s):  
Jian Feng Ma ◽  
Namiki Mitani ◽  
Sakiko Nagao ◽  
Saeko Konishi ◽  
Kazunori Tamai ◽  
...  
Keyword(s):  
Molecular Mapping ◽  
Uptake System ◽  
Transporter Gene ◽  
Silicon Transporter ◽  
Silicon Uptake

scholarly journals Functional Characterization of a Silicon Transporter Gene Implicated in Silicon Distribution in Barley

2012 ◽  
Vol 160 (3) ◽  
pp. 1491-1497 ◽  
Author(s):  
Naoki Yamaji ◽  
Yukako Chiba ◽  
Namiki Mitani-Ueno ◽  
Jian Feng Ma
Keyword(s):  
Functional Characterization ◽  
Transporter Gene ◽  
Silicon Transporter

scholarly journals Cloning of the trkAH gene cluster and characterization of the Trk K+-uptake system of Vibrio alginolyticus

Microbiology ◽  
1998 ◽  
Vol 144 (8) ◽  
pp. 2281-2289 ◽  
Author(s):  
T. Nakamura ◽  
N. Yamamuro ◽  
S. Stumpe ◽  
T. Unemoto ◽  
E. P. Bakker
Keyword(s):  
Gene Cluster ◽  
Vibrio Alginolyticus ◽  
Uptake System ◽  
K Uptake

scholarly journals Characterization of simultaneous uptake of xylose and glucose in Caldicellulosiruptor kronotskyensis for optimal hydrogen production

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Thitiwut Vongkampang ◽  
Krishnan Sreenivas ◽  
Jonathan Engvall ◽  
Carl Grey ◽  
Ed W. J. van Niel
Keyword(s):  
Glucose Uptake ◽  
Growth Patterns ◽  
Sugar Uptake ◽  
Uptake System ◽  
Sugar Mixtures ◽  
Xylose Uptake ◽  
Growth Profiles ◽  
High Concentrations ◽  
Xylose Transporter

Abstract Background Caldicellulosiruptor kronotskyensis has gained interest for its ability to grow on various lignocellulosic biomass. The aim of this study was to investigate the growth profiles of C. kronotskyensis in the presence of mixtures of glucose–xylose. Recently, we characterized a diauxic-like pattern for C. saccharolyticus on lignocellulosic sugar mixtures. In this study, we aimed to investigate further whether C. kronotskyensis has adapted to uptake glucose in the disaccharide form (cellobiose) rather than the monosaccharide (glucose). Results Interestingly, growth of C. kronotskyensis on glucose and xylose mixtures did not display diauxic-like growth patterns. Closer investigation revealed that, in contrast to C. saccharolyticus, C. kronotskyensis does not possess a second uptake system for glucose. Both C. saccharolyticus and C. kronotskyensis share the characteristics of preferring xylose over glucose. Growth on xylose was twice as fast (μmax = 0.57 h−1) as on glucose (μmax = 0.28 h−1). A study of the sugar uptake was made with different glucose–xylose ratios to find a kinetic relationship between the two sugars for transport into the cell. High concentrations of glucose inhibited xylose uptake and vice versa. The inhibition constants were estimated to be KI,glu = 0.01 cmol L−1 and KI,xyl = 0.001 cmol L−1, hence glucose uptake was more severely inhibited by xylose uptake. Bioinformatics analysis could not exclude that C. kronotskyensis possesses more than one transporter for glucose. As a next step it was investigated whether glucose uptake by C. kronotskyensis improved in the form of cellobiose. Indeed, cellobiose is taken up faster than glucose; nevertheless, the growth rate on each sugar remained similar. Conclusions C. kronotskyensis possesses a xylose transporter that might take up glucose at an inferior rate even in the absence of xylose. Alternatively, glucose can be taken up in the form of cellobiose, but growth performance is still inferior to growth on xylose. Therefore, we propose that the catabolism of C. kronotskyensis has adapted more strongly to pentose rather than hexose, thereby having obtained a specific survival edge in thermophilic lignocellulosic degradation communities.

scholarly journals Production and characterization of transgenic rice plants carrying a high-affinity nitrate transporter gene (OsNRT2.1)

2009 ◽  
Vol 59 (3) ◽  
pp. 237-243 ◽  
Author(s):  
Hisato Katayama ◽  
Mari Mori ◽  
Yoko Kawamura ◽  
Toshinori Tanaka ◽  
Masashi Mori ◽  
...  
Keyword(s):  
Transgenic Rice ◽  
Nitrate Transporter ◽  
Transporter Gene ◽  
Rice Plants ◽  
High Affinity ◽  
Transgenic Rice Plants ◽  
Nitrate Transporter Gene

Characterization of the monoamine uptake system in catecholamine storage vesicles isolated from a pheochromocytoma taken from a child

1984 ◽  
Vol 33 (14) ◽  
pp. 2245-2252 ◽  
Author(s):  
M.P. Roisin ◽  
M.F. Isambert ◽  
J.P. Henry ◽  
M. Guillot ◽  
G. Lenoir
Keyword(s):  
Uptake System ◽  
Monoamine Uptake ◽  
Storage Vesicles ◽  
Catecholamine Storage
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