scholarly journals Rapid Reaction Kinetics of Proline Dehydrogenase in the Multifunctional Proline Utilization A Protein

Biochemistry ◽  
2011 ◽  
Vol 51 (1) ◽  
pp. 511-520 ◽  
Author(s):  
Michael A. Moxley ◽  
Donald F. Becker
Keyword(s):  
Reaction Kinetics ◽  
Proline Dehydrogenase ◽  
Proline Utilization ◽  
Rapid Reaction ◽  
Proline Utilization A ◽  
Kinetics Of

scholarly journals Structural and functional studies of proline catabolic enzymes and human aldehyde dehydrogenases

2015 ◽  
Author(s):  
◽  
Min Luo
Keyword(s):  
Hot Spot ◽  
Gram Negative Bacteria ◽  
Proline Dehydrogenase ◽  
Aldehyde Dehydrogenases ◽  
Functional Studies ◽  
Gram Positive Bacteria ◽  
Catabolic Enzymes ◽  
Proline Catabolism ◽  
Proline Utilization ◽  
Proline Utilization A

Oxidation of amino acids, like proline catabolism, is a central part of energy metabolism. Proline is oxidized to glutamate by two enzymes: proline dehydrogenase (PRODH) and 1-pyrroline-5-carboxylate dehydrogenase (P5CDH). PRODH catalyzes the first reaction of proline to 1-pyrroline-5-carboxylate (P5C). P5C undergoes a non-enzymatic hydrolysis to glutamate semialdehyde (GSA), which is oxidized to glutamate by a NAD+- dependent enzyme P5CDH. PRODH and P5CDH are mono-functional enzymes in eukaryotes and Gram-positive bacteria; while in Gram-negative bacteria, the two enzymes are fused into one protein as two domains, known as proline utilization A (PutA). This dissertation work involved structural and functional studies of PRODH, P5CDH, PutA, and human aldehyde dehydrogenases (ALDHs). The results illuminated the substrate recognition for mono-functional PRODH and hot spot oligomerization mechanism for mono-functional P5CDH, also, demonstrated that diethylaminobenzaldehyde (DEAB) is a mechanism based inactivator for aldehyde dehydrogenase 7A1. Furthermore, the C-terminal domain found in PutAs, the only domain without any structural and functional information has been structurally and biochemically characterized.

scholarly journals Involvement of the β3-α3 Loop of the Proline Dehydrogenase Domain in Allosteric Regulation of Membrane Association of Proline Utilization A

Biochemistry ◽  
2013 ◽  
Vol 52 (26) ◽  
pp. 4482-4491 ◽  
Author(s):  
Weidong Zhu ◽  
Ashley M. Haile ◽  
Ranjan K. Singh ◽  
John D. Larson ◽  
Danielle Smithen ◽  
...  
Keyword(s):  
Allosteric Regulation ◽  
Membrane Association ◽  
Proline Dehydrogenase ◽  
Proline Utilization ◽  
Proline Utilization A

Impact Of Synthesis Route on the Water Oxidation Kinetics of Hematite Photoanodes

2020 ◽  
Author(s):  
Camilo A. Mesa ◽  
Ludmilla Steier ◽  
Benjamin Moss ◽  
Laia Francàs ◽  
James E. Thorne ◽  
...  
Keyword(s):  
Oxygen Vacancy ◽  
Reaction Kinetics ◽  
Water Oxidation ◽  
Oxidation Kinetics ◽  
Oxidation Reaction ◽  
Charge Accumulation ◽  
Optical Signals ◽  
Current Voltage ◽  
Voltage Performance ◽  
Kinetics Of

<p><i>Operando</i> spectroelectrochemical analysis is used to determine the water oxidation reaction kinetics for hematite photoanodes prepared using four different synthetic procedures. Whilst these photoanodes exhibit very different current / voltage performance, their underlying water oxidation kinetics are found to be almost invariant. Lower photoanode performance was found to correlate with the observation of optical signals indicative of charge accumulation in mid-gap oxygen vacancy states, indicating these states do not contribute directly to water oxidation.</p>

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