scholarly journals Evidence of the Participation of Remote Residues in the Catalytic Activity of Co-Type Nitrile Hydratase fromPseudomonas putida

Biochemistry ◽  
2011 ◽  
Vol 50 (22) ◽  
pp. 4923-4935 ◽  
Author(s):  
Heather R. Brodkin ◽  
Walter R. P. Novak ◽  
Amy C. Milne ◽  
J. Alejandro D’Aquino ◽  
N. M. Karabacak ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Nitrile Hydratase ◽  
Remote Residues

An anchoring residue adjacent to the substrate access tunnel entrance of nitrile hydratase directs its catalytic activity towards 3-cyanopyridine

2021 ◽  
Author(s):  
Zhongyi Cheng ◽  
Weimiao Zhang ◽  
Yuanyuan Xia ◽  
Dong Ma ◽  
Zhe-Min Zhou
Keyword(s):  
Catalytic Activity ◽  
Nitrile Hydratase ◽  
Salt Bridge ◽  
Tunnel Entrance

The βGlu50 residue located adjacent to the substrate access tunnel entrance of nitrile hydratase from Pseudonocardia thermophila JCM3095 acts as an anchoring residue. Breaking the salt bridge between β50 residue...

Novel catalytic activity of nitrile hydratase from Rhodococcus sp. N771

2008 ◽  
Vol 106 (2) ◽  
pp. 174-179 ◽  
Author(s):  
Kayoko Taniguchi ◽  
Kensuke Murata ◽  
Yoshihiko Murakami ◽  
Shunya Takahashi ◽  
Takemichi Nakamura ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Nitrile Hydratase ◽  
Rhodococcus Sp

scholarly journals Computational Design of Nitrile Hydratase from Pseudonocardia thermophila JCM3095 for Improved Thermostability

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4806
Author(s):  
Zhongyi Cheng ◽  
Yao Lan ◽  
Junling Guo ◽  
Dong Ma ◽  
Shijin Jiang ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Rational Design ◽  
Nitrile Hydratase ◽  
Elevated Temperatures ◽  
Residual Activity ◽  
Fold Increase ◽  
Computational Design ◽  
Site Directed Mutagenesis ◽  
Multiple Point ◽  
Substrate Affinity

High thermostability and catalytic activity are key properties for nitrile hydratase (NHase, EC 4.2.1.84) as a well-industrialized catalyst. In this study, rational design was applied to tailor the thermostability of NHase from Pseudonocardia thermophila JCM3095 (PtNHase) by combining FireProt server prediction and molecular dynamics (MD) simulation. Site-directed mutagenesis of non-catalytic residues provided by the rational design was subsequentially performed. The positive multiple-point mutant, namely, M10 (αI5P/αT18Y/αQ31L/αD92H/βA20P/βP38L/βF118W/βS130Y/βC189N/βC218V), was obtained and further analyzed. The Melting temperature (Tm) of the M10 mutant showed an increase by 3.2 °C and a substantial increase in residual activity of the enzyme at elevated temperatures was also observed. Moreover, the M10 mutant also showed a 2.1-fold increase in catalytic activity compared with the wild-type PtNHase. Molecular docking and MD simulations demonstrated better substrate affinity and improved thermostability for the mutant.

scholarly journals Post-translational modification is essential for catalytic activity of nitrile hydratase

2000 ◽  
Vol 9 (5) ◽  
pp. 1024-1030 ◽  
Author(s):  
Taku Murakami ◽  
Masaki Nojiri ◽  
Hiroshi Nakayama ◽  
Naoshi Dohmae ◽  
Koji Takio ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Nitrile Hydratase ◽  
Post Translational Modification

scholarly journals The Alpha Subunit of Nitrile Hydratase Is Sufficient for Catalytic Activity and Post-Translational Modification

Biochemistry ◽  
2014 ◽  
Vol 53 (24) ◽  
pp. 3990-3994 ◽  
Author(s):  
Micah T. Nelp ◽  
Andrei V. Astashkin ◽  
Linda A. Breci ◽  
Reid M. McCarty ◽  
Vahe Bandarian
Keyword(s):  
Catalytic Activity ◽  
Nitrile Hydratase ◽  
Alpha Subunit ◽  
Post Translational Modification

High resolution electron microscopy of lanthanum phosphate crystals

1978 ◽  
Vol 36 (1) ◽  
pp. 274-275
Author(s):  
J. C. Wheatley ◽  
J. M. Cowley
Keyword(s):  
Electron Microscopy ◽  
Catalytic Activity ◽  
High Resolution ◽  
Petroleum Industry ◽  
High Resolution Electron Microscopy ◽  
Lanthanum Phosphate ◽  
Good Catalyst ◽  
Resolution Electron ◽  
Good Catalytic Activity ◽  
Physical And Chemical

Rare-earth phosphates are of particular interest because of their catalytic properties associated with the hydrolysis of many aromatic chlorides in the petroleum industry. Lanthanum phosphates (LaPO4) which have been doped with small amounts of copper have shown increased catalytic activity (1). However the physical and chemical characteristics of the samples leading to good catalytic activity are not known.Many catalysts are amorphous and thus do not easily lend themselves to methods of investigation which would include electron microscopy. However, the LaPO4, crystals are quite suitable samples for high resolution techniques.The samples used were obtained from William L. Kehl of Gulf Research and Development Company. The electron microscopy was carried out on a JEOL JEM-100B which had been modified for high resolution microscopy (2). Standard high resolution techniques were employed. Three different sample types were observed: 669A-1-5-7 (poor catalyst), H-L-2 (good catalyst) and 27-011 (good catalyst).

The design and catalytic performance of molybdenum active sites on an MCM-41 framework for the aerobic oxidation of 5-hydroxymethylfurfural to 2,5-diformylfuran

2019 ◽  
Vol 9 (3) ◽  
pp. 811-821 ◽  
Author(s):  
Zhao-Meng Wang ◽  
Li-Juan Liu ◽  
Bo Xiang ◽  
Yue Wang ◽  
Ya-Jing Lyu ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Active Sites ◽  
Aerobic Oxidation ◽  
Catalytic Performance ◽  
Mcm 41

The catalytic activity decreases as –(SiO)3Mo(OH)(O) > –(SiO)2Mo(O)2 > –(O)4–MoO.

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