Novel catalytic activity of nitrile hydratase from Rhodococcus sp. N771

2008 ◽  
Vol 106 (2) ◽  
pp. 174-179 ◽  
Author(s):  
Kayoko Taniguchi ◽  
Kensuke Murata ◽  
Yoshihiko Murakami ◽  
Shunya Takahashi ◽  
Takemichi Nakamura ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Nitrile Hydratase ◽  
Rhodococcus Sp

Purification and Properties of the Nitrile Hydratase of a New Strain of Rhodococcus sp.

1991 ◽  
Vol 146 (2) ◽  
pp. 89-98 ◽  
Author(s):  
M.R. Kaakeh ◽  
J.-L. Legras ◽  
R. Duran ◽  
Ch.K.N. Chan Kwo Chion ◽  
A. Arnaud ◽  
...  
Keyword(s):  
Nitrile Hydratase ◽  
Purification And Properties ◽  
Rhodococcus Sp

An anchoring residue adjacent to the substrate access tunnel entrance of nitrile hydratase directs its catalytic activity towards 3-cyanopyridine

2021 ◽  
Author(s):  
Zhongyi Cheng ◽  
Weimiao Zhang ◽  
Yuanyuan Xia ◽  
Dong Ma ◽  
Zhe-Min Zhou
Keyword(s):  
Catalytic Activity ◽  
Nitrile Hydratase ◽  
Salt Bridge ◽  
Tunnel Entrance

The βGlu50 residue located adjacent to the substrate access tunnel entrance of nitrile hydratase from Pseudonocardia thermophila JCM3095 acts as an anchoring residue. Breaking the salt bridge between β50 residue...

Photosensitive Nitrile Hydratase from Rhodococcus sp. N-771.

1992 ◽  
Vol 672 (1 Enzyme Engine) ◽  
pp. 29-36 ◽  
Author(s):  
JUN HONDA ◽  
TERUYUKI NAGAMUNE ◽  
YOSHITAKA TERATANI ◽  
AKIRA HIRATA ◽  
HIROYUKI SASABE ◽  
...  
Keyword(s):  
Nitrile Hydratase ◽  
Rhodococcus Sp

scholarly journals Computational Design of Nitrile Hydratase from Pseudonocardia thermophila JCM3095 for Improved Thermostability

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4806
Author(s):  
Zhongyi Cheng ◽  
Yao Lan ◽  
Junling Guo ◽  
Dong Ma ◽  
Shijin Jiang ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Rational Design ◽  
Nitrile Hydratase ◽  
Elevated Temperatures ◽  
Residual Activity ◽  
Fold Increase ◽  
Computational Design ◽  
Site Directed Mutagenesis ◽  
Multiple Point ◽  
Substrate Affinity

High thermostability and catalytic activity are key properties for nitrile hydratase (NHase, EC 4.2.1.84) as a well-industrialized catalyst. In this study, rational design was applied to tailor the thermostability of NHase from Pseudonocardia thermophila JCM3095 (PtNHase) by combining FireProt server prediction and molecular dynamics (MD) simulation. Site-directed mutagenesis of non-catalytic residues provided by the rational design was subsequentially performed. The positive multiple-point mutant, namely, M10 (αI5P/αT18Y/αQ31L/αD92H/βA20P/βP38L/βF118W/βS130Y/βC189N/βC218V), was obtained and further analyzed. The Melting temperature (Tm) of the M10 mutant showed an increase by 3.2 °C and a substantial increase in residual activity of the enzyme at elevated temperatures was also observed. Moreover, the M10 mutant also showed a 2.1-fold increase in catalytic activity compared with the wild-type PtNHase. Molecular docking and MD simulations demonstrated better substrate affinity and improved thermostability for the mutant.

scholarly journals Post-translational modification is essential for catalytic activity of nitrile hydratase

2000 ◽  
Vol 9 (5) ◽  
pp. 1024-1030 ◽  
Author(s):  
Taku Murakami ◽  
Masaki Nojiri ◽  
Hiroshi Nakayama ◽  
Naoshi Dohmae ◽  
Koji Takio ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Nitrile Hydratase ◽  
Post Translational Modification

scholarly journals Draft Genome Sequence of Rhodococcus sp. Strain M8, Which Can Degrade a Broad Range of Nitriles

2018 ◽  
Vol 6 (6) ◽  
pp. e01526-17 ◽  
Author(s):  
Andrey D. Novikov ◽  
Konstantin V. Lavrov ◽  
Artem S. Kasianov ◽  
Tatyana V. Gerasimova ◽  
Alexander S. Yanenko
Keyword(s):  
Genome Sequence ◽  
Nitrile Hydratase ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Contaminated Sites ◽  
Degrading Bacterium ◽  
Rhodococcus Sp

ABSTRACT Rhodococcus sp. strain M8 is a nitrile-degrading bacterium isolated from acrylonitrile-contaminated sites. This strain produces the enzymes for sequential nitrile degradation, cobalt-type nitrile hydratase, and amidase in large amounts. Its draft genome sequence, announced here, has an estimated size of 6.3 Mbp.

Crystallization of a photosensitive nitrile hydratase from Rhodococcus sp. N-771

1991 ◽  
Vol 220 (2) ◽  
pp. 221-222 ◽  
Author(s):  
Teruyuki Nagamune ◽  
Jun Honda ◽  
Won-Dai Cho ◽  
Nobuo Kamiya ◽  
Yoshitaka Teratani ◽  
...  
Keyword(s):  
Nitrile Hydratase ◽  
Rhodococcus Sp
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