SARS CoV Main Proteinase:  The Monomer−Dimer Equilibrium Dissociation Constant

Biochemistry ◽  
2006 ◽  
Vol 45 (49) ◽  
pp. 14632-14641 ◽  
Author(s):  
Vito Graziano ◽  
William J. McGrath ◽  
Lin Yang ◽  
Walter F. Mangel
Keyword(s):  
Dissociation Constant ◽  
Equilibrium Dissociation Constant ◽  
Equilibrium Dissociation

Ontogeny of gastric mucosal muscarinic receptor and sensitivity to carbachol

1984 ◽  
Vol 246 (5) ◽  
pp. G550-G555
Author(s):  
E. R. Seidel ◽  
L. R. Johnson
Keyword(s):  
Dissociation Constant ◽  
Cholinergic Receptor ◽  
Equilibrium Dissociation Constant ◽  
Muscarinic Cholinergic Receptor ◽  
Fetal Rat ◽  
Gastric Lumen ◽  
Basal Acid ◽  
Muscarinic Cholinergic ◽  
Equilibrium Dissociation ◽  
Apparent Equilibrium

Development of the muscarinic cholinergic receptor and sensitivity of oxyntic gland mucosa to a muscarinic agonist were studied in rats of various ages. The gastric lumen of the fetal rat at the 20th day of gestation contained a statistically significant amount of basal pepsin, which increased log linearly over the first 30 days of life. Carbachol was effective in stimulating the secretion of pepsin as early as 12 h after birth. Basal acid could be measured in the gastric lumen 12 h after birth. The secretion of basal acid increased log linearly over the first 30 days of life. Carbachol was an effective secretagogue even in the fetal rat. The density of the muscarinic cholinergic receptor of the adult rat oxyntic gland mucosa was 99.3 fmol/mg protein with an apparent equilibrium dissociation constant for quinuclidinyl benzilate of 0.40 nM. The receptor was well developed even in the fetal rat, which bound 79.6 fmol/mg protein with an apparent equilibrium dissociation constant of 0.26 nM. Except for immediately after birth, receptor density was maintained between 70 and 90% of the adult level over the first 30 days of life. These results suggest that cholinergic regulation of gastric acid and pepsin secretion is probably functional by either late gestation or at least immediately after birth.

Ouabain binding to cultured vascular smooth muscle cells of the spontaneously hypertensive rat

1986 ◽  
Vol 250 (6) ◽  
pp. C948-C954 ◽  
Author(s):  
L. Hopp ◽  
F. Khalil ◽  
H. Tamura ◽  
M. Kino ◽  
B. M. Searle ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Dissociation Constant ◽  
Vascular Smooth Muscle Cells ◽  
Equilibrium Dissociation Constant ◽  
Rat Strains ◽  
Spontaneously Hypertensive ◽  
Na Pump ◽  
Equilibrium Dissociation

The binding of ouabain and K+ to the Na+ pump were analyzed in serially passed cultured vascular smooth muscle cells (VSMCs) originating from spontaneously hypertensive (SH), Wistar-Kyoto (WKY), and American Wistar (W) rats. Our techniques have utilized analyses of displacement of [3H]ouabain by both unlabeled ouabain and K+ from specific binding sites on the VSMCs. We have found that each of the VSMC preparations from the three rat strains appeared to demonstrate one population of specific ouabain receptors (Na+ pumps); the number of Na+ pump units (mean +/- SE, expressed as 10(5) units/cell; number of observations indicated in parentheses) of both the SH and WKY rats was significantly lower than the number of Na+ pump units of W rat VSMCs [SH: 3.00 +/- 0.02 (231), WKY: 2.87 +/- 0.05 (245), and W: 3.62 +/- 0.04 (225)]; the equilibrium dissociation constant values (microM) for ouabain in VSMCs of SH and WKY rats were similar but were significantly higher than that of VSMCs derived from W rats [SH: 4.69 +/- 0.09 (231), WKY: 4.57 +/- 0.12 (245), and W: 3.69 +/- 0.17 (225)]; and among the VSMCs originating from the three rat strains, the apparent equilibrium dissociation constant value for K+ (mM) was the lowest in those of the SH rat [1.04 +/- 0.003 (143), compared with VSMCs of the WKY rat [1.54 +/- 0.006 (135)] and W rat [1.19 +/- 0.003 (136)]. Our previous studies have demonstrated increased passive Na+ and K+ transport rate constants of SH rat VSMCs compared with either W or WKY rat cells. These findings suggest the possibility of higher permeabilities of the SH cells.(ABSTRACT TRUNCATED AT 250 WORDS)

Platelet 3H Ketanserin Binding in Migraine

Cephalalgia ◽  
2001 ◽  
Vol 21 (5) ◽  
pp. 567-572 ◽  
Author(s):  
R Shukla ◽  
VK Khanna ◽  
S Pradeep ◽  
M Husain ◽  
R Tandon ◽  
...  
Keyword(s):  
Dissociation Constant ◽  
Clinical Features ◽  
Binding Sites ◽  
Scatchard Analysis ◽  
Healthy Controls ◽  
Equilibrium Dissociation Constant ◽  
Binding Characteristics ◽  
Number Of Binding Sites ◽  
Equilibrium Dissociation ◽  
Dissociation Constant Kd

Platelet 3H ketanserin binding was studied in 33 patients of migraine and 30 healthy controls. The binding characteristics: equilibrium dissociation constant (Kd) and maximal number of binding sites (Bmax) determined by Scatchard analysis revealed a significant decrease in Kd and no change in Bmax in migraine cases. No correlation was observed between the Kd and Bmax with the clinical features of migraine. The findings of the present study show that there is a decreased affinity of platelet 5-HT2 receptors in migraine.

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