Role of Aromatic Interactions in Amyloid Formation by Peptides Derived from Human Amylin†

Biochemistry ◽  
2004 ◽  
Vol 43 (50) ◽  
pp. 15901-15908 ◽  
Author(s):  
Sylvia M. Tracz ◽  
Andisheh Abedini ◽  
Miles Driscoll ◽  
Daniel P. Raleigh
Keyword(s):  
Amyloid Formation ◽  
Aromatic Interactions ◽  
Human Amylin

scholarly journals Amyloidogenicity and cytotoxicity of des-Lys-1 human amylin provides insight into amylin self-assembly and highlights the difficulties of defining amyloidogenicity

2019 ◽  
Vol 32 (2) ◽  
pp. 87-93 ◽  
Author(s):  
Kyung-Hoon Lee ◽  
Alexander Zhyvoloup ◽  
Daniel Raleigh
Keyword(s):  
Self Assembly ◽  
Amyloid Formation ◽  
Wild Type ◽  
Islet Amyloid ◽  
High Charge Density ◽  
Human Amylin ◽  
Phosphate Buffered Saline

Abstract The polypeptide amylin is responsible for islet amyloid in type 2 diabetes, a process which contributes to β-cell death in the disease. The role of the N-terminal region of amylin in amyloid formation is relatively unexplored, although removal of the disulfide bridged loop between Cys-2 and Cys-7 accelerates amyloid formation. We examine the des Lys-1 variant of human amylin (h-amylin), a variant which is likely produced in vivo. Lys-1 is a region of high charge density in the h-amylin amyloid fiber. The des Lys-1 polypeptide forms amyloid on the same time scale as wild-type amylin in phosphate buffered saline, but does so more rapidly in Tris. The des Lys-1 variant is somewhat less toxic to cultured INS cells than wild type. The implications for the in vitro mechanism of amyloid formation and for comparative analysis of amyloidogenicity are discussed.

Carbohydrate-Aromatic Interactions: The Role of Curvature on XH···π Interactions

2010 ◽  
Vol 114 (12) ◽  
pp. 4313-4324 ◽  
Author(s):  
R. Mahesh Kumar ◽  
M. Elango ◽  
V. Subramanian
Keyword(s):  
Π Interactions ◽  
Aromatic Interactions

scholarly journals Mutational Analysis of Preamyloid Intermediates: The Role of His-Tyr Interactions in Islet Amyloid Formation

2014 ◽  
Vol 106 (7) ◽  
pp. 1520-1527 ◽  
Author(s):  
Ling-Hsien Tu ◽  
Arnaldo L. Serrano ◽  
Martin T. Zanni ◽  
Daniel P. Raleigh
Keyword(s):  
Mutational Analysis ◽  
Amyloid Formation ◽  
Islet Amyloid

Characterization of Amyloid Formation by Glucagon-Like Peptides: Role of Basic Residues in Heparin-Mediated Aggregation

Biochemistry ◽  
2013 ◽  
Vol 52 (49) ◽  
pp. 8800-8810 ◽  
Author(s):  
Narendra Nath Jha ◽  
A. Anoop ◽  
Srivastav Ranganathan ◽  
Ganesh M. Mohite ◽  
Ranjith Padinhateeri ◽  
...  
Keyword(s):  
Amyloid Formation

Role of Oxidative Stress in Protein Misfolding and/or Amyloid Formation

2010 ◽  
pp. 615-630 ◽  
Author(s):  
Johanna C. Scheinost ◽  
Daniel P. Witter ◽  
Grant E. Boldt ◽  
Paul Wentworth
Keyword(s):  
Oxidative Stress ◽  
Protein Misfolding ◽  
Amyloid Formation
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