Real-Time Measurement of in Vitro Peptide Binding to Soluble HLA-A*0201 by Fluorescence Polarization

Biochemistry ◽  
2004 ◽  
Vol 43 (46) ◽  
pp. 14852-14863 ◽  
Author(s):  
Rico Buchli ◽  
Rodney S. VanGundy ◽  
Heather D. Hickman-Miller ◽  
Christopher F. Giberson ◽  
Wilfried Bardet ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescence Polarization ◽  
Peptide Binding ◽  
Time Measurement ◽  
Real Time Measurement ◽  
Soluble Hla

scholarly journals Real-time measurement of adenosine and ATP release in the central nervous system

2020 ◽  
Author(s):  
Nicholas Dale
Keyword(s):  
Real Time ◽  
Motor Pattern ◽  
Atp Release ◽  
In Vitro Models ◽  
Time Measurement ◽  
Pattern Generation ◽  
Neural Function ◽  
Adaptive Changes ◽  
Real Time Measurement

AbstractThis brief review recounts how, stimulated by the work of Geoff Burnstock, I developed biosensors that allowed direct real-time measurement of ATP and adenosine during neural function. The initial impetus to create an adenosine biosensor came from trying to understand how ATP and adenosine-modulated motor pattern generation in the frog embryo spinal cord. Early biosensor measurements demonstrated slow accumulation of adenosine during motor activity. Subsequent application of these biosensors characterized real-time release of adenosine in in vitro models of brain ischaemia, and this line of work has recently led to clinical measurements of whole blood purine levels in patients undergoing carotid artery surgery or stroke. In parallel, the wish to understand the role of ATP signalling in the chemosensory regulation of breathing stimulated the development of ATP biosensors. This revealed that release of ATP from the chemosensory areas of the medulla oblongata preceded adaptive changes in breathing, triggered adaptive changes in breathing via activation of P2 receptors, and ultimately led to the discovery of connexin26 as a channel that mediates CO2-gated release of ATP from cells.

scholarly journals Direct, real-time measurement of shear stress-induced nitric oxide produced from endothelial cells in vitro

Nitric Oxide ◽  
2010 ◽  
Vol 23 (4) ◽  
pp. 335-342 ◽  
Author(s):  
Allison M. Andrews ◽  
Dov Jaron ◽  
Donald G. Buerk ◽  
Patrick L. Kirby ◽  
Kenneth A. Barbee
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Shear Stress ◽  
Real Time ◽  
Time Measurement ◽  
Real Time Measurement

scholarly journals Use of the Real Time xCelligence System for Purposes of Medical Microbiology

2012 ◽  
Vol 61 (3) ◽  
pp. 191-197 ◽  
Author(s):  
ADAM F. JUNKA ◽  
ADRIANA JANCZURA ◽  
DANUTA SMUTNICKA ◽  
BEATA MĄCZYŃSKA ◽  
ANNA SECEWICZ ◽  
...  
Keyword(s):  
Real Time ◽  
Biofilm Formation ◽  
Reference Strain ◽  
Morphological Changes ◽  
Chinese Hamster ◽  
Eukaryotic Cell ◽  
Time Measurement ◽  
Bacterial Biofilm ◽  
Real Time Measurement

Roche's xCelligence impedance-measuring instrument is one of a few commercially available systems of such type. According to the best knowledge of authors, instrument was tested so far only for eukaryotic cell research. The aim of this work was to estimate xCELLigence suitability for the microbiological tests, including (i) measurement of morphological changes in eukaryotic cells as a result of bacterial toxin activity, (ii) measurement of bacterial biofilm formation and (iii) impact of antiseptics on the biofilm structure. To test the infuence of bacterial LT enterotoxin on eukaryotic cell lines, Chinese Hamster Ovary (CHO) cell line and reference strain Escherichia coli ATTC 35401 were used. To investigate Roche's instrument ability to measure biofilm formation and impact of antiseptics on its development, Staphylococcus aureus ATTC6538 reference strain was used. The data generated during the experiments indicate excellent ability of xCelligence instrument to detect cytopathic effect caused by bacterial LT endotoxin and to detect staphylococcal biofilm formation. However, interpretation of the results obtained during real-time measurement of antiseptic's bactericidal activity against staphylococcal biofilm, caused many difficulties. xCelligence instrument can be used for real-time monitoring of morphological changes in CHO cells treated with bacterial LT enterotoxin and for real-time measurement of staphylococcal biofilm formation in vitro. Further investigation is necessary to confirm suitability of system to analyze antiseptic's antimicrobial activity against biofilm in vitro.

Real-time measurement of in vitro flow by Fourier-domain color Doppler optical coherence tomography

2004 ◽  
Vol 29 (2) ◽  
pp. 171 ◽  
Author(s):  
Rainer A. Leitgeb ◽  
Leopold Schmetterer ◽  
Christoph K. Hitzenberger ◽  
Adolf F. Fercher ◽  
Fatma Berisha ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Real Time ◽  
Color Doppler ◽  
Time Measurement ◽  
Fourier Domain ◽  
Optical Coherence ◽  
Real Time Measurement ◽  
Doppler Optical Coherence Tomography

Real-time measurement of in-vitro and in-vivo blood flow with Fourier domain optical coherence tomography

2004 ◽  
Author(s):  
Rainer Leitgeb ◽  
Leopold Schmetterer ◽  
Wolfgang Drexler ◽  
Fatmire Berisha ◽  
Christoph K. Hitzenberger ◽  
...  
Keyword(s):  
Blood Flow ◽  
Optical Coherence Tomography ◽  
Real Time ◽  
Time Measurement ◽  
Fourier Domain ◽  
Optical Coherence ◽  
Real Time Measurement
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