Sequence-Selective Interaction of the Minor-Groove Interstrand Cross-Linking Agent SJG-136 with Naked and Cellular DNA:  Footprinting and Enzyme Inhibition Studies†

Biochemistry ◽  
2005 ◽  
Vol 44 (11) ◽  
pp. 4135-4147 ◽  
Author(s):  
Chris Martin ◽  
Tom Ellis ◽  
Claire J. McGurk ◽  
Terence C. Jenkins ◽  
John A. Hartley ◽  
...  
Keyword(s):  
Enzyme Inhibition ◽  
Minor Groove ◽  
Cross Linking ◽  
Dna Footprinting ◽  
Selective Interaction ◽  
Cellular Dna ◽  
Inhibition Studies

Mycobacterial DNA GyrB Inhibitors: Ligand Based Pharmacophore Modelling and In Vitro Enzyme Inhibition Studies

2014 ◽  
Vol 14 (17) ◽  
pp. 1990-2005 ◽  
Author(s):  
Shalini Saxena ◽  
Janupally Renuka ◽  
Variam Jeankumar ◽  
Perumal Yogeeswari ◽  
Dharmarajan Sriram
Keyword(s):  
Enzyme Inhibition ◽  
Pharmacophore Modelling ◽  
Inhibition Studies

scholarly journals Use of the photoaffinity cross-linking agent N-hydroxysuccinimidyl-4-azidosalicylic acid to characterize salivary-glycoprotein-bacterial interactions

1986 ◽  
Vol 234 (1) ◽  
pp. 43-48 ◽  
Author(s):  
E J Bergey ◽  
M J Levine ◽  
M S Reddy ◽  
S D Bradway ◽  
I Al-Hashimi
Keyword(s):  
Polyacrylamide Gel Electrophoresis ◽  
Specific Interaction ◽  
Gel Filtration ◽  
Cross Linking ◽  
Oral Streptococci ◽  
Bacterial Surface ◽  
Acetylneuraminic Acid ◽  
Streptococcus Sanguis ◽  
Surface Examination ◽  
Inhibition Studies

The present study has utilized the iodinatable cross-linking agent N-hydroxysuccinimidyl-4-azidosalicylic acid (ASA) to examine the specific interaction between the proline-rich glycoprotein (PRG) of human parotid saliva and Streptococcus sanguis G9B. The binding of 125I-ASA-PRG to Streptococcus sanguis G9B displayed saturation kinetics, reversibility and was inhibited by unlabelled PRG. Inhibition studies with other glycoproteins and saccharides indicated that binding was mediated by a bacterial adhesin with specificity towards N-acetylneuraminic acid, galactose, and N-acetylgalactosamine. After cross-linking, the 125I-ASA-PRG-adhesin complex could be extracted with SDS and separated from uncoupled 125I-ASA-PRG by gel filtration on Sepharose CL-6B. Approx. 1% of the 125I-ASA-PRG was cross-linked to the bacterial surface. Examination of the 125I-ASA-PRG-adhesin complex by SDS/polyacrylamide-gel electrophoresis/fluorography on 5% -(w/v)-polyacrylamide gels revealed that PRG was bound to two bacterial components. These findings support our previous suggestion that human salivary glycoproteins can specifically interact with oral streptococci and that these interactions occur between the glycoprotein's carbohydrate units and lectin(s) on the bacterial cell surface.

scholarly journals Potential Effects of Corneal Cross-Linking upon the Limbus

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Johnny E. Moore ◽  
Davide Schiroli ◽  
C. B. Tara Moore
Keyword(s):  
Stem Cells ◽  
Potential Risk ◽  
Ocular Surface ◽  
Ex Vivo ◽  
Uv Exposure ◽  
Cross Linking ◽  
Clinical Complications ◽  
Cellular Dna ◽  
Pathological Consequence

Corneal cross-linking is nowadays the most used strategy for the treatment of keratoconus and recently it has been exploited for an increasing number of different corneal pathologies, from other ectatic disorders to keratitis. The safety of this technique has been widely assessed, but clinical complications still occur. The potential effects of cross-linking treatment upon the limbus are incompletely understood; it is important therefore to investigate the effect of UV exposure upon the limbal niche, particularly as UV is known to be mutagenic to cellular DNA and the limbus is where ocular surface tumors can develop. The risk of early induction of ocular surface cancer is undoubtedly rare and has to date not been published other than in one case after cross-linking. Nevertheless it is important to further assess, understand, and reduce where possible any potential risk. The aim of this review is to summarize all the reported cases of a pathological consequence for the limbal cells, possibly induced by cross-linking UV exposure, the studies donein vitroorex vivo, the theoretical bases for the risks due to UV exposure, and which aspects of the clinical treatment may produce higher risk, along with what possible mechanisms could be utilized to protect the limbus and the delicate stem cells present within it.

scholarly journals Cellular pharmacology of novel C8-linked anthramycin-based sequence-selective DNA minor groove cross-linking agents

1994 ◽  
Vol 70 (1) ◽  
pp. 48-53 ◽  
Author(s):  
M Smellie ◽  
LR Kelland ◽  
DE Thurston ◽  
RL Souhami ◽  
JA Hartley
Keyword(s):  
Minor Groove ◽  
Cross Linking ◽  
Dna Minor Groove ◽  
Cellular Pharmacology

scholarly journals Synthesis, Spectral and Enzyme Inhibition Studies on N-Aralkyl/ Aryl Sulfonated Derivatives of Commercially Available Paroxetine

2014 ◽  
Vol 26 (1) ◽  
pp. 93-98
Author(s):  
Asia Siddiqa ◽  
Aziz-ur-Rehman ◽  
Muhammad Athar Abbasi ◽  
Shahid Rasool ◽  
Ghulam Hussain ◽  
...  
Keyword(s):  
Enzyme Inhibition ◽  
Inhibition Studies ◽  
Derivatives Of

Isolation and Enzyme-Inhibition Studies of the Chemical Constituents fromAjuga bracteosa

2007 ◽  
Vol 4 (1) ◽  
pp. 72-83 ◽  
Author(s):  
Naheed Riaz ◽  
Sarfraz A. Nawaz ◽  
Naveen Mukhtar ◽  
Abdul Malik ◽  
Nighat Afza ◽  
...  
Keyword(s):  
Enzyme Inhibition ◽  
Chemical Constituents ◽  
Inhibition Studies

scholarly journals Phytochemical Composition and Enzyme Inhibition Studies of Buxus papillosa C.K. Schneid

Processes ◽  
2020 ◽  
Vol 8 (7) ◽  
pp. 757
Author(s):  
Hammad Saleem ◽  
Thet Thet Htar ◽  
Rakesh Naidu ◽  
Gokhan Zengin ◽  
Marcello Locatelli ◽  
...  
Keyword(s):  
Enzyme Inhibition ◽  
Skin Diseases ◽  
Research Work ◽  
Tentative Identification ◽  
Chemical Profiling ◽  
Phytochemical Composition ◽  
In Vitro Bioassays ◽  
Inhibition Studies ◽  
Wide Usage

The current research work is an endeavor to study the chemical profiling and enzyme-inhibition potential of different polarity solvent (n-hexane, dichloromethane—DCM and methanol—MeOH) extracts from the aerial and stem parts of Buxus papillosa C.K. Schneid. All the extracts were analyzed for HPLC-PDA phenolic quantification, while both (aerial and stem) DCM extracts were studied for UHPLC-MS phytochemical composition. The inhibitory activity against the clinically important enzymes having crucial role in different pathologies like skin diseases (tyrosinase), inflammatory problems (lipoxygenase—LOX) and diabetes mellitus (α-amylase) were studied using standard in vitro bioassays. The DCM extracts upon UHPLC-MS analysis conducted in both negative and positive ionization modes has led to the tentative identification of 52 important secondary metabolites. Most of these belonged to the alkaloid, flavonoid, phenolic and triterpenoid classes. The HPLC-PDA polyphenolic quantification identified the presence of 10 phenolic compounds. Catechin was present in significant amounts in aerial-MeOH (7.62 ± 0.45 μg/g extract) and aerial-DCM (2.39 ± 0.51-μg/g extract) extracts. Similarly, higher amounts of epicatechin (2.76 ± 0.32-μg/g extract) and p-hydroxybenzoic acid (1.06 ± 0.21 μg/g extract) were quantified in aerial-DCM and stem-MeOH extracts, respectively. Likewise, all the extracts exhibited moderate inhibition against all the tested enzymes. These findings explain the wide usage of this plant in folklore medicine and suggest that it could be further studied as an origin of novel bioactive phytocompounds and for the designing of new pharmaceuticals.

ENZYME INHIBITION STUDIES WITH DICHLOROBENZOPRIM: A NOVEL LIPOPHILIC ANTIFOLATE

1990 ◽  
Vol 42 (S1) ◽  
pp. 95P-95P
Author(s):  
R J Griffin ◽  
M F G Stevens ◽  
P A Lambert
Keyword(s):  
Enzyme Inhibition ◽  
Inhibition Studies
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