The Crystal Structure of the Y66L Variant of Green Fluorescent Protein Supports a Cyclization−Oxidation−Dehydration Mechanism for Chromophore Maturation†,‡

Biochemistry ◽  
2004 ◽  
Vol 43 (15) ◽  
pp. 4464-4472 ◽  
Author(s):  
Matthew A. Rosenow ◽  
Holly A. Huffman ◽  
Marlene E. Phail ◽  
Rebekka M. Wachter
Keyword(s):  
Crystal Structure ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Dehydration Mechanism ◽  
Green Fluorescent

scholarly journals The structure of a GFP-based antibody (fluorobody) to TLH, a toxin fromVibrio parahaemolyticus

2015 ◽  
Vol 71 (7) ◽  
pp. 913-918 ◽  
Author(s):  
Yaoguang Chen ◽  
Xiaocheng Huang ◽  
Rongzhi Wang ◽  
Shihua Wang ◽  
Ning Shi
Keyword(s):  
Crystal Structure ◽  
Green Fluorescent Protein ◽  
Vibrio Parahaemolyticus ◽  
Fluorescent Protein ◽  
Hybrid Molecule ◽  
Unit Cell Parameters ◽  
Cell Parameters ◽  
Food Borne ◽  
Vapour Diffusion ◽  
Green Fluorescent

A fluorobody is a manmade hybrid molecule that is composed of green fluorescent protein (GFP) and a fragment of antibody, which combines the affinity and specificity of an antibody with the visibility of a GFP. It is able to provide a real-time indication of binding while avoiding the use of tags and secondary binding reagents. Here, the expression, purification and crystal structure of a recombinant fluorobody for TLH (thermolabile haemolysin), a toxin from the lethal food-borne disease bacteriumVibrio parahaemolyticus, are presented. This is the first structure of a fluorobody to be reported. Crystals belonging to space groupP43212, with unit-cell parametersa=b= 63.35,c = 125.90 Å, were obtained by vapour diffusion in hanging drops and the structure was refined to anRfreeof 16.7% at 1.5 Å resolution. The structure shows a CDR loop of the antibody on the GFP scaffold.

scholarly journals A Green Fluorescent Protein Containing a QFG Tri-Peptide Chromophore: Optical Properties and X-Ray Crystal Structure

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e47331 ◽  
Author(s):  
Jion M. Battad ◽  
Daouda A. K. Traore ◽  
Emma Byres ◽  
Jamie Rossjohn ◽  
Rodney J. Devenish ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Optical Properties ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
X Ray ◽  
Green Fluorescent

Crystal Structure and Photodynamic Behavior of the Blue Emission Variant Y66H/Y145F of Green Fluorescent Protein†

Biochemistry ◽  
1997 ◽  
Vol 36 (32) ◽  
pp. 9759-9765 ◽  
Author(s):  
Rebekka M. Wachter ◽  
Brett A. King ◽  
Roger Heim ◽  
Karen Kallio ◽  
Roger Y. Tsien ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Blue Emission ◽  
Green Fluorescent

Crystal Structure of the Aequorea victoria Green Fluorescent Protein

Science ◽  
1996 ◽  
Vol 273 (5280) ◽  
pp. 1392-1395 ◽  
Author(s):  
M. Orm  ◽  
A. B. Cubitt ◽  
K. Kallio ◽  
L. A. Gross ◽  
R. Y. Tsien ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Aequorea Victoria ◽  
Green Fluorescent

Effects of the benzoxazole group on green fluorescent protein chromophore crystal structure and solid state photophysics

2016 ◽  
Vol 4 (14) ◽  
pp. 2793-2801 ◽  
Author(s):  
Abdelhamid Ghodbane ◽  
W. Brett Fellows ◽  
John R. Bright ◽  
Debashree Ghosh ◽  
Nathalie Saffon ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Green Fluorescent Protein ◽  
Solid State ◽  
Fluorescent Protein ◽  
Aggregation Induced Emission ◽  
Emission Enhancement ◽  
Green Fluorescent ◽  
Unusual Type

Most of the conjugates exhibited aggregation-induced emission enhancement, probably involving a unusual type of excimer.

The 1.7 Å Crystal Structure of Dronpa: A Photoswitchable Green Fluorescent Protein

2006 ◽  
Vol 364 (2) ◽  
pp. 213-224 ◽  
Author(s):  
Pascal G. Wilmann ◽  
Kristina Turcic ◽  
Jion M. Battad ◽  
Matthew C.J. Wilce ◽  
Rodney J. Devenish ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Green Fluorescent

Crystal Structure of the pH-Dependent Green Fluorescent Protein WasCFP with a Tryptophan-Based Chromophore at an Extremely Low pH of 2.0

2018 ◽  
Vol 44 (6) ◽  
pp. 640-644
Author(s):  
N. V. Pletneva ◽  
R. G. Efremov ◽  
E. A. Goryacheva ◽  
I. V. Artemyev ◽  
S. F. Arkhipova ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Low Ph ◽  
Ph Dependent ◽  
Green Fluorescent

scholarly journals Crystal structure and spectral studies of green fluorescent protein (GFP) chromophore analogue ethyl 2-[(4Z)-(6-hydroxy naphthalen-2-yl) methylene)-2-methyl-5-oxo-4,5-di hydro-1H-imidazol-1-yl] acetate

2019 ◽  
Vol 10 (2) ◽  
pp. 175-179
Author(s):  
Anisha Puthuvakkal ◽  
Kochunnoonny Manoj
Keyword(s):  
Crystal Structure ◽  
Green Fluorescent Protein ◽  
Live Cell Imaging ◽  
Fluorescent Protein ◽  
Spectral Studies ◽  
Triclinic Space Group ◽  
Non Covalent Interactions ◽  
Gfp Chromophore ◽  
Green Fluorescent ◽  
Covalent Interactions

Synthetically modified green fluorescent protein chromophore derivative was prepared, its crystal structure and spectral properties were studied. Crystal data for C19H18N2O4: triclinic, space group P-1 (no. 2), a = 8.2506(17) Å, b = 11.934(2) Å, c = 17.461(4) Å, α = 102.89(3)°,   β = 94.62(3)°, γ = 96.68(3)°, V = 1654.5(6) Å3, Z = 4, T = 173(2) K, μ(MoKα) = 0.096 mm-1, Dcalc = 1.358 g/cm3, 7227 reflections measured (4.722° ≤ 2Θ ≤ 53.996°), 7227 unique (Rint = 0.0453, Rsigma = 0.0662) which were used in all calculations. The final R1 was 0.0561 (I > 2σ(I)) and wR2 was 0.1658 (all data). The single crystal structure showed, the benzylidine moiety adopts Z-conformation in solid state and the molecules were associated by various O−H···O and C−H···O non-covalent interactions. The UV absorption-emission spectral analysis indicated that a significant red shift of emission observed at alkaline pH indicating its utility for live cell imaging applications.

Sign in / Sign up

Export Citation Format

Share Document