Functional Analysis of Genetic Mutations in Nucleotide Binding Domain 2 of the Human Retina Specific ABC Transporter†

Biochemistry ◽  
2003 ◽  
Vol 42 (36) ◽  
pp. 10683-10696 ◽  
Author(s):  
Esther E. Biswas-Fiss
Keyword(s):  
Functional Analysis ◽  
Abc Transporter ◽  
Nucleotide Binding ◽  
Binding Domain ◽  
Genetic Mutations ◽  
Human Retina ◽  
Nucleotide Binding Domain ◽  
Nucleotide Binding Domain 2

scholarly journals Resistance to Bacitracin in Bacillus subtilis: Unexpected Requirement of the BceAB ABC Transporter in the Control of Expression of Its Own Structural Genes

2007 ◽  
Vol 189 (23) ◽  
pp. 8636-8642 ◽  
Author(s):  
Remi Bernard ◽  
Annick Guiseppi ◽  
Marc Chippaux ◽  
Maryline Foglino ◽  
François Denizot
Keyword(s):  
Signal Transduction ◽  
Bacillus Subtilis ◽  
Abc Transporter ◽  
Defense Mechanism ◽  
Response Regulator ◽  
Nucleotide Binding ◽  
Binding Domain ◽  
Nucleotide Binding Domain ◽  
Signal Transduction System ◽  
Native Form

ABSTRACT The Bacillus subtilis BceAB ABC transporter involved in a defense mechanism against bacitracin is composed of a membrane-spanning domain and a nucleotide-binding domain. Induction of the structural bceAB genes requires the BceR response regulator and the BceS histidine kinase of a signal transduction system. However, despite the presence of such a transduction system and of bacitracin, no transcription from an unaltered bceA promoter is observed in cells lacking the BceAB transporter. Expression in trans of the BceAB transporter in these bceAB cells restores the transcription from the bceA promoter. Cells possessing a mutated nucleotide-binding domain of the transporter are also no longer able to trigger transcription from the bceA promoter in the presence of bacitracin, although the mutated ABC transporter is still bound to the membrane. In these cells, expression of the bceA promoter can no longer be detected, indicating that the ABC transporter not only must be present in the cell membrane, but also must be expressed in a native form for the induction of the bceAB genes. Several hypotheses are discussed to explain the simultaneous need for bacitracin, a native signal transduction system, and an active BceAB ABC transporter to trigger transcription from the bceA promoter.

scholarly journals Thermodynamics of the ATPase Cycle of GlcV, the Nucleotide-Binding Domain of the Glucose ABC Transporter ofSulfolobus solfataricus†

Biochemistry ◽  
2006 ◽  
Vol 45 (50) ◽  
pp. 15056-15067 ◽  
Author(s):  
Monika G. Pretz ◽  
Sonja-Verena Albers ◽  
Gea Schuurman-Wolters ◽  
Robert Tampé ◽  
Arnold J. M. Driessen ◽  
...  
Keyword(s):  
Abc Transporter ◽  
Nucleotide Binding ◽  
Binding Domain ◽  
Nucleotide Binding Domain
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