Spectroscopic Studies of DNA and ATP Binding to Human Polynucleotide Kinase:  Evidence for a Ternary Complex†

Biochemistry ◽  
2003 ◽  
Vol 42 (41) ◽  
pp. 12077-12084 ◽  
Author(s):  
Rajam S. Mani ◽  
Feridoun Karimi-Busheri ◽  
Mesfin Fanta ◽  
Carol E. Cass ◽  
Michael Weinfeld
Keyword(s):  
Ternary Complex ◽  
Spectroscopic Studies ◽  
Atp Binding ◽  
Polynucleotide Kinase

Physical Properties of Human Polynucleotide Kinase:  Hydrodynamic and Spectroscopic Studies†

Biochemistry ◽  
2001 ◽  
Vol 40 (43) ◽  
pp. 12967-12973 ◽  
Author(s):  
Rajam S. Mani ◽  
Feridoun Karimi-Busheri ◽  
Carol E. Cass ◽  
Michael Weinfeld
Keyword(s):  
Physical Properties ◽  
Spectroscopic Studies ◽  
Polynucleotide Kinase

scholarly journals Metal Ion Chelates as Surrogates of Nucleobases for the Recognition of Nucleic Acid Sequences: ThePd2+Complex of 2,6-Bis(3,5-dimethylpyrazol-1-yl)purine Riboside

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Sharmin Taherpour ◽  
Tuomas Lönnberg
Keyword(s):  
Nucleic Acid ◽  
Metal Ion ◽  
Ternary Complex ◽  
Spectroscopic Studies ◽  
Complementary Dna ◽  
Micromolar Concentration ◽  
Oligonucleotide Hybridization ◽  
Dna Strand ◽  
Cd Studies ◽  
Uv Melting

A 2,6-bis(3,5-dimethylpyrazol-1-yl)purine ribonucleoside has been prepared and incorporated as a conventionally protected phosphoramidite into a 9-mer 2′-O-methyl oligoribonucleotide. According to 1H NMR spectroscopic studies, this nucleoside forms with Pd2+and uridine a ternary complex that is stable at a micromolar concentration range. CD spectroscopic studies on oligonucleotide hybridization, in turn, suggest that the Pd2+chelate of this artificial nucleoside, when incorporated in a 2′-O-methyl-RNA oligomer, is able to recognize thymine within an otherwise complementary DNA strand. The duplex containing thymidine opposite to the artificial nucleoside turned out to be somewhat more resistant to heating than its counterpart containing 2′-deoxycytidine in place of thymidine, but only in the presence of Pd2+. According to UV-melting measurements, replacement of 2′-O-methyladenosine with the artificial nucleoside markedly enhances hybridization with a DNA target, irrespective of the identity of the opposite base and the presence of Pd2+. With the thymidine containing DNA target, theTmvalue is 2–4°C higher than with targets containing any other nucleoside opposite to the artificial nucleoside, but the dependence on Pd2+is much less clear than in the case of the CD studies.

scholarly journals Alignment of the protein substrate hairpin along the SecA two-helix finger primes protein transport in Escherichia coli

2017 ◽  
Vol 114 (35) ◽  
pp. 9343-9348 ◽  
Author(s):  
Qi Zhang ◽  
Sudipta Lahiri ◽  
Tithi Banerjee ◽  
Zhongmou Sun ◽  
Donald Oliver ◽  
...  
Keyword(s):  
Signal Peptide ◽  
Bound States ◽  
Ternary Complex ◽  
Protein Transport ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Spectroscopic Studies ◽  
Ternary Complexes ◽  
Protein Substrate ◽  
Key Residues

A conserved hairpin-like structure comprised of a signal peptide and early mature region initiates protein transport across the SecY or Sec61α channel in Bacteria or Archaea and Eukarya, respectively. When and how this initiator substrate hairpin forms remains a mystery. Here, we have used the bacterial SecA ATPase motor protein and SecYEG channel complex to address this question. Engineering of a functional miniprotein substrate onto the end of SecA allowed us to efficiently form ternary complexes with SecYEG for spectroscopic studies. Förster resonance energy transfer mapping of key residues within this ternary complex demonstrates that the protein substrate adopts a hairpin-like structure immediately adjacent to the SecA two-helix finger subdomain before channel entry. Comparison of ADP and ATP-γS–bound states shows that the signal peptide partially inserts into the SecY channel in the latter state. Our study defines a unique preinsertion intermediate state where the SecA two-helix finger appears to play a role in both templating the substrate hairpin at the channel entrance and promoting its subsequent ATP-dependent insertion.

Immunolocalization and funtional role of scavenger receptor BI (SR-BI) and ATP-binding cassette A1 (ABC1)

2001 ◽  
Vol 120 (5) ◽  
pp. A678-A678
Author(s):  
I SUC ◽  
M BENDAYAN ◽  
E DELVIN ◽  
L BRISSETTE ◽  
C GAROFALO ◽  
...  
Keyword(s):  
Scavenger Receptor ◽  
Atp Binding ◽  
Scavenger Receptor Bi ◽  
Atp Binding Cassette

Spectroscopic studies of Pd (II) complexes with cysteine and its di- and tripeptides

1987 ◽  
Vol 84 ◽  
pp. 443-448 ◽  
Author(s):  
Teresa Kowalik ◽  
Brigitte Decock-Le Reverend ◽  
Claude Loucheux ◽  
Damien Ficheux ◽  
Henryk Kozlowski
Keyword(s):  
Spectroscopic Studies

MÖSSBAUER SPECTROSCOPIC STUDIES OF DIMENSIONALITY AND SPIN REDUCTION EFFECTS IN THE ANTIFERROMAGNETIC SYSTEMS : (AF)n-FeF3

1980 ◽  
Vol 41 (C1) ◽  
pp. C1-187-C1-188 ◽  
Author(s):  
G. P. Gupta ◽  
J. A. Baines ◽  
D. M. Cooper ◽  
D. P.E. Dickson ◽  
C. E. Johnson
Keyword(s):  
Spectroscopic Studies
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