scholarly journals Metal Ion Chelates as Surrogates of Nucleobases for the Recognition of Nucleic Acid Sequences: ThePd2+Complex of 2,6-Bis(3,5-dimethylpyrazol-1-yl)purine Riboside

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Sharmin Taherpour ◽  
Tuomas Lönnberg
Keyword(s):  
Nucleic Acid ◽  
Metal Ion ◽  
Ternary Complex ◽  
Spectroscopic Studies ◽  
Complementary Dna ◽  
Micromolar Concentration ◽  
Oligonucleotide Hybridization ◽  
Dna Strand ◽  
Cd Studies ◽  
Uv Melting

A 2,6-bis(3,5-dimethylpyrazol-1-yl)purine ribonucleoside has been prepared and incorporated as a conventionally protected phosphoramidite into a 9-mer 2′-O-methyl oligoribonucleotide. According to 1H NMR spectroscopic studies, this nucleoside forms with Pd2+and uridine a ternary complex that is stable at a micromolar concentration range. CD spectroscopic studies on oligonucleotide hybridization, in turn, suggest that the Pd2+chelate of this artificial nucleoside, when incorporated in a 2′-O-methyl-RNA oligomer, is able to recognize thymine within an otherwise complementary DNA strand. The duplex containing thymidine opposite to the artificial nucleoside turned out to be somewhat more resistant to heating than its counterpart containing 2′-deoxycytidine in place of thymidine, but only in the presence of Pd2+. According to UV-melting measurements, replacement of 2′-O-methyladenosine with the artificial nucleoside markedly enhances hybridization with a DNA target, irrespective of the identity of the opposite base and the presence of Pd2+. With the thymidine containing DNA target, theTmvalue is 2–4°C higher than with targets containing any other nucleoside opposite to the artificial nucleoside, but the dependence on Pd2+is much less clear than in the case of the CD studies.

scholarly journals Reliable microspotting methodology for peptide-nucleic acid layers with high hybridization efficiency on gold SPR imaging chips

2015 ◽  
Vol 7 (15) ◽  
pp. 6077-6082 ◽  
Author(s):  
L. Simon ◽  
G. Lautner ◽  
R. E. Gyurcsányi
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Gold Surfaces ◽  
Complementary Dna ◽  
Spr Imaging ◽  
Hybridization Efficiency ◽  
Dna Strand

Microspotting of HS-PNA strands prehybridized with a complementary DNA strand onto gold surfaces results in PNA layers with excellent hybridization efficiency.

scholarly journals New Brightener for Zn-Fe Alloy Plating from Sulphate Bath

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
B. M. Praveen ◽  
T. V. Venkatesha
Keyword(s):  
Grain Size ◽  
Metal Ion ◽  
Condensation Product ◽  
Spectroscopic Studies ◽  
Sem Images ◽  
Alloy Electrodeposition ◽  
Fine Grained ◽  
Polarization Studies ◽  
Uv Visible ◽  
Sulphate Bath

Zn-Fe alloy electrodeposition was carried out in the presence of condensation product 2-{[(1E)-(3,4-dimethoxyphenyl)methylidene]amino}-3-hydroxypropanoic acid formed between veratraldehyde and serine in acid sulphate bath. Hull cell was used for optimizing the operating parameters and bath constituents. During deposition, the potential was shifted towards cathodic direction in the presence of addition agents and brightener. The polarization studies show that deposition taking place in basic bath and optimum bath was 1.08 and 1.15 V, respectively. Current efficiency and throwing power were reached around 85% and 26%, respectively. The SEM images of bright deposit indicated its fine-grained nature and appreciable reduction in the grain size. XRD studies have showed that the grain size of the deposit generated from optimum bath was 16 nm. UV-visible spectroscopic studies confirm the formation of complex between metal ion and brightener.

Mercury Electrodes in Nucleic Acid Electrochemistry: Sensitive Analytical Tools and Probes of DNA Structure. A Review

2004 ◽  
Vol 69 (4) ◽  
pp. 715-747 ◽  
Author(s):  
Miroslav Fojta
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Double Helix ◽  
Dna Structure ◽  
Electrochemical Analysis ◽  
Label Free ◽  
Helix Formation ◽  
Mercury Electrodes ◽  
Dna Strand ◽  
Dna Double Helix

This review is devoted to applications of mercury electrodes in the electrochemical analysis of nucleic acids and in studies of DNA structure and interactions. At the mercury electrodes, nucleic acids yield faradaic signals due to redox processes involving adenine, cytosine and guanine residues, and tensammetric signals due to adsorption/desorption of polynucleotide chains at the electrode surface. Some of these signals are highly sensitive to DNA structure, providing information about conformation changes of the DNA double helix, formation of DNA strand breaks as well as covalent or non-covalent DNA interactions with small molecules (including genotoxic agents, drugs, etc.). Measurements at mercury electrodes allow for determination of small quantities of unmodified or electrochemically labeled nucleic acids. DNA-modified mercury electrodes have been used as biodetectors for DNA damaging agents or as detection electrodes in DNA hybridization assays. Mercury film and solid amalgam electrodes possess similar features in the nucleic acid analysis to mercury drop electrodes. On the contrary, intrinsic (label-free) DNA electrochemical responses at other (non-mercury) solid electrodes cannot provide information about small changes of the DNA structure. A review with 188 references.

scholarly journals A nucleic acid strand displacement system for the multiplexed detection of tuberculosis-specific mRNA using quantum dots

Nanoscale ◽  
2016 ◽  
Vol 8 (19) ◽  
pp. 10087-10095 ◽  
Author(s):  
H. D. Gliddon ◽  
P. D. Howes ◽  
M. Kaforou ◽  
M. Levin ◽  
M. M. Stevens
Keyword(s):  
Quantum Dots ◽  
Nucleic Acid ◽  
Strand Displacement ◽  
Multiplexed Detection ◽  
Mrna Detection ◽  
Dna Strand Displacement ◽  
Displacement System ◽  
Dna Strand ◽  
Specific Mrna

On the development of a novel multiplexed assay for Tuberculosis-specific mRNA detection using DNA strand displacement and quantum dots.

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