Role of Protein−Protein Bridging Interactions on Cooperative Assembly of DNA-Bound CRP−CytR−CRP Complex and Regulation of theEscherichia ColiCytR Regulon†

Biochemistry ◽  
2003 ◽  
Vol 42 (13) ◽  
pp. 3812-3825 ◽  
Author(s):  
Mayy Chahla ◽  
John Wooll ◽  
Thomas M. Laue ◽  
Nghia Nguyen ◽  
Donald F. Senear
Keyword(s):  
Cooperative Assembly

scholarly journals Anti-cooperative assembly of the SRP19 and SRP68/72 components of the signal recognition particle

2008 ◽  
Vol 415 (3) ◽  
pp. 429-437 ◽  
Author(s):  
Tuhin Subhra Maity ◽  
Howard M. Fried ◽  
Kevin M. Weeks
Keyword(s):  
Electrostatic Interactions ◽  
Signal Recognition Particle ◽  
Cooperative Binding ◽  
Signal Recognition ◽  
Parallel Orientation ◽  
Domain Interactions ◽  
Cooperative Assembly ◽  
Srp Rna ◽  
Antagonistic Interactions

The mammalian SRP (signal recognition particle) represents an important model for the assembly and role of inter-domain interactions in complex RNPs (ribonucleoproteins). In the present study we analysed the interdependent interactions between the SRP19, SRP68 and SRP72 proteins and the SRP RNA. SRP72 binds the SRP RNA largely via non-specific electrostatic interactions and enhances the affinity of SRP68 for the RNA. SRP19 and SRP68 both bind directly and specifically to the same two RNA helices, but on opposite faces and at opposite ends. SRP19 binds at the apices of helices 6 and 8, whereas the SRP68/72 heterodimer binds at the three-way junction involving RNA helices 5, 6 and 8. Even though both SRP19 and SRP68/72 stabilize a similar parallel orientation for RNA helices 6 and 8, these two proteins bind to the RNA with moderate anti-cooperativity. Long-range anti-cooperative binding by SRP19 and SRP68/72 appears to arise from stabilization of distinct conformations in the stiff intervening RNA scaffold. Assembly of large RNPs is generally thought to involve either co-operative or energetically neutral interactions among components. By contrast, our findings emphasize that antagonistic interactions can play significant roles in assembly of multi-subunit RNPs.

Cooperative Assembly of Metal Nitrate and Citric Acid with Block Copolymers: Role of Carbonate Conversion Temperature on the Mesostructure of Ordered Porous Oxides

2015 ◽  
Vol 119 (22) ◽  
pp. 12138-12148 ◽  
Author(s):  
Michael C. Burroughs ◽  
Sarang M. Bhaway ◽  
Pattarasai Tangvijitsakul ◽  
Kevin A. Cavicchi ◽  
Mark D. Soucek ◽  
...  
Keyword(s):  
Citric Acid ◽  
Block Copolymers ◽  
Metal Nitrate ◽  
Porous Oxides ◽  
Cooperative Assembly ◽  
Ordered Porous ◽  
Conversion Temperature

scholarly journals Role of the pre-initiation complex in Mediator recruitment and dynamics

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Elisabeth R Knoll ◽  
Z Iris Zhu ◽  
Debasish Sarkar ◽  
David Landsman ◽  
Randall H Morse
Keyword(s):  
Rna Polymerase Ii ◽  
Gene Activation ◽  
Mediator Complex ◽  
Gene Promoters ◽  
Initiation Complex ◽  
Yeast Saccharomyces Cerevisiae ◽  
Pol Ii ◽  
Genome Wide ◽  
Cooperative Assembly

The Mediator complex stimulates the cooperative assembly of a pre-initiation complex (PIC) and recruitment of RNA Polymerase II (Pol II) for gene activation. The core Mediator complex is organized into head, middle, and tail modules, and in budding yeast (Saccharomyces cerevisiae), Mediator recruitment has generally been ascribed to sequence-specific activators engaging the tail module triad of Med2-Med3-Med15 at upstream activating sequences (UASs). We show that yeast lacking Med2-Med3-Med15 are viable and that Mediator and PolII are recruited to promoters genome-wide in these cells, albeit at reduced levels. To test whether Mediator might alternatively be recruited via interactions with the PIC, we examined Mediator association genome-wide after depleting PIC components. We found that depletion of Taf1, Rpb3, and TBP profoundly affected Mediator association at active gene promoters, with TBP being critical for transit of Mediator from UAS to promoter, while Pol II and Taf1 stabilize Mediator association at proximal promoters.

Cooperative assembly of Janus particles and amphiphilic oligomers: the role of Janus balance

Nanoscale ◽  
2019 ◽  
Vol 11 (15) ◽  
pp. 7221-7228 ◽  
Author(s):  
Jiawei Li ◽  
Junfeng Wang ◽  
Qiang Yao ◽  
Kai Yu ◽  
Youguo Yan ◽  
...  
Keyword(s):  
Spatial Localization ◽  
Janus Particles ◽  
Janus Nanoparticles ◽  
Hybrid Assemblies ◽  
Cooperative Assembly

The spatial localization of Janus nanoparticles within hybrid assemblies can be controlled by regulating the Janus balance of nanoparticles.

Role of dactinomycin in the improved survival of children with Wilms' tumor

JAMA ◽  
1966 ◽  
Vol 195 (12) ◽  
pp. 1005-1009 ◽  
Author(s):  
D. J. Fernbach
Keyword(s):  
Wilms Tumor

Role of the allergist in diagnosis and management of patients with uveitis

JAMA ◽  
1966 ◽  
Vol 195 (3) ◽  
pp. 167-172 ◽  
Author(s):  
T. E. Van Metre
Keyword(s):  
Diagnosis And Management

The power of norms to sway fused group members

2018 ◽  
Vol 41 ◽  
Author(s):  
Winnifred R. Louis ◽  
Craig McGarty ◽  
Emma F. Thomas ◽  
Catherine E. Amiot ◽  
Fathali M. Moghaddam
Keyword(s):  
Evolutionary Anthropology ◽  
Normative Systems ◽  
Violent Extremism ◽  
Identity Fusion ◽  
Group Members

AbstractWhitehouse adapts insights from evolutionary anthropology to interpret extreme self-sacrifice through the concept of identity fusion. The model neglects the role of normative systems in shaping behaviors, especially in relation to violent extremism. In peaceful groups, increasing fusion will actually decrease extremism. Groups collectively appraise threats and opportunities, actively debate action options, and rarely choose violence toward self or others.

Elaborating the role of reflection and individual differences in the study of folk-economic beliefs

2018 ◽  
Vol 41 ◽  
Author(s):  
Kevin Arceneaux
Keyword(s):  
Decision Making ◽  
Individual Differences ◽  
Cognitive Processes ◽  
Evolutionary History ◽  
Behavioral Responses ◽  
History Of ◽  
Economic Beliefs

AbstractIntuitions guide decision-making, and looking to the evolutionary history of humans illuminates why some behavioral responses are more intuitive than others. Yet a place remains for cognitive processes to second-guess intuitive responses – that is, to be reflective – and individual differences abound in automatic, intuitive processing as well.

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