Unusual Benefits of Macromolecular Shielding by Polyethylene Glycol for Reactions at the Diffusional Limit:  The Case of Factor VIIai and Tissue Factor†

Biochemistry ◽  
2002 ◽  
Vol 41 (52) ◽  
pp. 15820-15825 ◽  
Author(s):  
Matthew Stone ◽  
Stephen B. Harvey ◽  
Walter Kisiel ◽  
Donald Foster ◽  
Gary L. Nelsestuen
Keyword(s):  
Polyethylene Glycol ◽  
Tissue Factor

Anticancer Therapy by Tumor Vessel Infarction with Polyethylene Glycol Conjugated Retargeted Tissue Factor

2013 ◽  
Vol 56 (6) ◽  
pp. 2337-2347 ◽  
Author(s):  
Christian Schwöppe ◽  
Caroline Zerbst ◽  
Max Fröhlich ◽  
Christoph Schliemann ◽  
Torsten Kessler ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Tissue Factor ◽  
Anticancer Therapy ◽  
Tumor Vessel

Immunocytochemical detection of hepatic carbohydrate metabolic enzymes: Improved resolution with polyethylene glycol embedding and visio-bond semithin sections

1992 ◽  
Vol 50 (1) ◽  
pp. 792-793
Author(s):  
Kuixiong Gao ◽  
Randal E. Morris ◽  
Bruce F. Giffin ◽  
Robert R. Cardell
Keyword(s):  
Polyethylene Glycol ◽  
Glycogen Phosphorylase ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Glycogen Synthase ◽  
Metabolic Enzymes ◽  
Silver Stain ◽  
Accurate Localization ◽  
Immunocytochemical Detection ◽  
Semithin Sections ◽  
Parenchymal Cells

Several enzymes are involved in the regulation of anabolic and catabolic pathways of carbohydrate metabolism in liver parenchymal cells. The lobular distribution of glycogen synthase (GS), phosphoenolpyruvate carboxykinase (PEPCK) and glycogen phosphorylase (GP) was studied by immunocytochemistry using cryosections of normal fed and fasted rat liver. Since sections of tissue embedded in polyethylene glycol (PEG) show good morphological preservation and increased detectability for immunocytochemical localization of antigenic sites, and semithin sections of Visio-Bond (VB) embedded tissue provide higher resolution of cellular structure, we applied these techniques and immunogold-silver stain (IGSS) for a more accurate localization of hepatic carbohydrate metabolic enzymes.

Directional observation on lipid of male nectary of Lindera megaphylla hemsl. by the rapid embedding method with polyethylene glycol (PEG)

1990 ◽  
Vol 48 (3) ◽  
pp. 718-719
Author(s):  
Dai Dalin ◽  
Guo Jianmin
Keyword(s):  
Electron Microscope ◽  
Polyethylene Glycol ◽  
Chemical Reaction ◽  
Traditional Method ◽  
Osmium Tetroxide ◽  
Unsaturated Lipid ◽  
Embedding Method ◽  
Long Period ◽  
New Methods

Lipid cytochemistry has not yet advanced far at the EM level. A major problem has been the loss of lipid during dehydration and embedding. Although the adoption of glutaraldehyde and osmium tetroxide accelerate the chemical reaction of lipid and osmium tetroxide can react on the double bouds of unsaturated lipid to from the osmium black, osmium tetroxide can be reduced in saturated lipid and subsequently some of unsaturated lipid are lost during dehydration. In order to reduce the loss of lipid by traditional method, some researchers adopted a few new methods, such as the change of embedding procedure and the adoption of new embedding media, to solve the problem. In a sense, these new methods are effective. They, however, usually require a long period of preparation. In this paper, we do research on the fiora nectary strucure of lauraceae by the rapid-embedding method wwith PEG under electron microscope and attempt to find a better method to solve the problem mentioned above.

Polyethylene glycol-mediated direct gene transfer in Nicotiana spp.

1992 ◽  
Vol 85 (2) ◽  
pp. 345-351 ◽  
Author(s):  
Gokarna B. Gharti-Chhetri ◽  
Wichai Cherdshewasart ◽  
Jocelyne Dewulf ◽  
Michel Jacobs ◽  
loan Negrutiu
Keyword(s):  
Polyethylene Glycol ◽  
Gene Transfer ◽  
Direct Gene Transfer ◽  
Direct Gene
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