Rotational-Echo Double Resonance Characterization of the Effects of Vancomycin on Cell Wall Synthesis inStaphylococcus aureus†

Biochemistry ◽  
2002 ◽  
Vol 41 (43) ◽  
pp. 13053-13058 ◽  
Author(s):  
Lynette Cegelski ◽  
Sung Joon Kim ◽  
Andrew W. Hing ◽  
Daniel R. Studelska ◽  
Robert D. O'Connor ◽  
...  
Keyword(s):  
Cell Wall ◽  
Double Resonance ◽  
Cell Wall Synthesis ◽  
Rotational Echo Double Resonance

Cloning and Characterization of psu1+, a New Essential Fission Yeast Gene Involved in Cell Wall Synthesis

1999 ◽  
Vol 262 (2) ◽  
pp. 368-374 ◽  
Author(s):  
Kazuo Omi ◽  
Hikaru Sonoda ◽  
Kiyoshi Nagata ◽  
Kenji Sugita
Keyword(s):  
Cell Wall ◽  
Fission Yeast ◽  
Cell Wall Synthesis ◽  
Yeast Gene

scholarly journals Characterization of the Aspergillus fumigatus phosphomannose isomerase Pmi1 and its impact on cell wall synthesis and morphogenesis

Microbiology ◽  
2009 ◽  
Vol 155 (10) ◽  
pp. 3281-3293 ◽  
Author(s):  
Wenxia Fang ◽  
Xiaoying Yu ◽  
Bin Wang ◽  
Hui Zhou ◽  
Haomiao Ouyang ◽  
...  
Keyword(s):  
Cell Wall ◽  
Aspergillus Fumigatus ◽  
Energy Production ◽  
Mutant Cell ◽  
Phosphomannose Isomerase ◽  
Cell Wall Synthesis ◽  
High Concentration ◽  
Human Fungal Pathogen ◽  
Sugar Chains

Phosphomannose isomerase (PMI) is an enzyme catalysing the interconversion of mannose 6-phosphate (Man-6-P) and fructose 6-phosphate (Fru-6-P). The reaction catalysed by PMI is the first committed step in the synthesis of mannose-containing sugar chains and provides a link between glucose metabolism and mannosylation. In this study, the pmi1 gene was identified to encode PMI in the human fungal pathogen Aspergillus fumigatus. Characterization of A. fumigatus Pmi1 expressed in Escherichia coli revealed that this PMI mainly catalysed the conversion of Fru-6-P to Man-6-P and that its binding affinity for Man-6-P was similar to that of yeast PMIs, but different to those of PMIs from bacteria or animals. Loss of pmi1 was lethal unless Man was provided in the growth medium. However, a Δpmi1 mutant cell showed a significantly reduced growth rate at a high concentration of Man. Biochemical analysis revealed that both inadequate and replete Man led to an accumulation of intracellular Man-6-P and a reduction in the amount of α-glucan in the cell wall. Uncoupling of the link between energy production and glycosylation by deletion of the pmi1 gene led to phenotypes such as defects in cell wall integrity, abnormal morphology and reduced conidiation. Our results reveal that PMI activity is essential for viability and plays a central regulatory role in both cell wall synthesis and energy production in A. fumigatus.

Rotational-Echo Double Resonance Characterization of Vancomycin Binding Sites inStaphylococcus aureus†

Biochemistry ◽  
2002 ◽  
Vol 41 (22) ◽  
pp. 6967-6977 ◽  
Author(s):  
Sung Joon Kim ◽  
Lynette Cegelski ◽  
Daniel R. Studelska ◽  
Robert D. O'Connor ◽  
Anil K. Mehta ◽  
...  
Keyword(s):  
Binding Sites ◽  
Double Resonance ◽  
Rotational Echo Double Resonance

scholarly journals Characterization of Interfacial Structure in Polymer-Fullerene Bulk Heterojunctions via C13 {H2} Rotational Echo Double Resonance NMR

2018 ◽  
Vol 121 (2) ◽  
Author(s):  
R. C. Nieuwendaal ◽  
D. M. DeLongchamp ◽  
L. J. Richter ◽  
C. R. Snyder ◽  
R. L. Jones ◽  
...  
Keyword(s):  
Double Resonance ◽  
Interfacial Structure ◽  
Bulk Heterojunctions ◽  
Rotational Echo Double Resonance
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