An Efficient High-Throughput Resonance Assignment Procedure for Structural Genomics and Protein Folding Research by NMR

Biochemistry ◽  
2001 ◽  
Vol 40 (49) ◽  
pp. 14727-14735 ◽  
Author(s):  
Neel S. Bhavesh ◽  
Sanjay C. Panchal ◽  
R. V. Hosur
Keyword(s):  
Protein Folding ◽  
High Throughput ◽  
Resonance Assignment ◽  
Structural Genomics

Automated seeding for the optimization of crystal quality

2010 ◽  
Vol 43 (4) ◽  
pp. 752-756 ◽  
Author(s):  
Sahir Khurshid ◽  
Lesley F. Haire ◽  
Naomi E. Chayen
Keyword(s):  
High Throughput ◽  
Structural Genomics ◽  
Optimization Method ◽  
Crystal Quality ◽  
Routine Manner

With the advent of structural genomics a variety of crystallization techniques have been automated and applied to high-throughput pipelines, yet seeding, which is the most common and successful optimization method, is still being performed predominantly manually. The aim of this study was to devise simple automated seeding techniques that can be applied in a routine manner using existing robots and not requiring special tools. Two alternative protocols for automated seeding experiments are described. One involves the delivery of microcrystals from stock to target wells using the robot dispensing tip as a seeding tool. The second harnesses an animal whisker as the seeding tool. Larger and better ordered crystals were obtained using both techniques.

scholarly journals NIGMS PSI:Biology Initiative ‐ Enabling High‐Throughput Structural Biology and Structural Genomics

2010 ◽  
Vol 24 (S1) ◽  
Author(s):  
Peter C. Preusch ◽  
Ravi Basavappa ◽  
Jean Chin ◽  
Charles Edmonds ◽  
Paula Flicker ◽  
...  
Keyword(s):  
Structural Biology ◽  
High Throughput ◽  
Structural Genomics

RIKEN structural genomics beamlines at the SPring-8; high throughput protein crystallography with automated beamline operation

2006 ◽  
Vol 7 (1) ◽  
pp. 15-22 ◽  
Author(s):  
Go Ueno ◽  
Hiroyuki Kanda ◽  
Raita Hirose ◽  
Koh Ida ◽  
Takashi Kumasaka ◽  
...  
Keyword(s):  
High Throughput ◽  
Structural Genomics ◽  
Protein Crystallography

scholarly journals Analyzing Protein Folding by High-throughput Simulations

2013 ◽  
Vol 104 (2) ◽  
pp. 398a ◽  
Author(s):  
Claude Sinner ◽  
Benjamin Lutz ◽  
Abhinav Verma ◽  
Alexander Schug
Keyword(s):  
Protein Folding ◽  
High Throughput

Cloning grills: High throughput cloning for structural genomics

2004 ◽  
Vol 5 (1/2) ◽  
pp. 59-61 ◽  
Author(s):  
Christopher Mehlin ◽  
Erica E. Boni ◽  
Jamie Andreyka ◽  
Richard W. Terry
Keyword(s):  
High Throughput ◽  
Structural Genomics
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