Effect of Androgen on the Incorporation of Orotic Acid-6-14C into the Ribonucleic Acids and Free Nucleotides of Mouse Kidney*

Biochemistry ◽  
1966 ◽  
Vol 5 (5) ◽  
pp. 1696-1701 ◽  
Author(s):  
Charles D. Kochakian ◽  
John Hill
Keyword(s):  
Orotic Acid ◽  
Mouse Kidney ◽  
Ribonucleic Acids ◽  
Free Nucleotides

THE METABOLISM OF EXOGENOUS RIBONUCLEIC ACIDS INJECTED INTO MICE

1965 ◽  
Vol 43 (7) ◽  
pp. 949-958 ◽  
Author(s):  
S. Sved
Keyword(s):  
Protein Synthesis ◽  
Amino Acid ◽  
Orotic Acid ◽  
The Body ◽  
Ribonucleic Acids ◽  
Exogenous Rna ◽  
Free Nucleotides ◽  
Liver And Kidney ◽  
Labeled Rna ◽  
The Brain

The fate of RNA administered intravenously has been investigated in mice and rats, using yeast RNA uniformly labeled with C14. Much of the administered radioactivity was found in the CO2 exhaled within 5 hours, the rest being distributed mostly between the free nucleotides and carbohydrates of the nine tissues tested. Brain showed consistently low values in all chemical fractions. Administration of large amounts of RNA over a period of 5 days caused an increase in the incorporation of radioactivity into the nucleic acids of liver and kidney and into the amino acid pool of the body. A slight increase in protein synthesis in the brain was also noted. From results obtained by studying the rate of appearance of the radioactivity in the CO2 after injections of labeled RNA, ribose, orotic acid, and RNA mixed with unlabeled ribose, it was concluded that most of the exogenous RNA, when injected, is rapidly degraded before re-utilization of some of its components.

scholarly journals Increased synthesis of low-molecular-weight nuclear ribonucleic acids of rat liver after γ irradiation and hepatectomy

1975 ◽  
Vol 146 (2) ◽  
pp. 357-360 ◽  
Author(s):  
A Fónagy ◽  
E J Hidvégi
Keyword(s):  
Molecular Weight ◽  
Partial Hepatectomy ◽  
Rat Liver ◽  
Orotic Acid ◽  
Low Molecular Weight ◽  
Polyacrylamide Gels ◽  
Γ Irradiation ◽  
Ribonucleic Acids

Incorporation of [3H]orotic acid into low-molecular-weight nRNA of rat liver, fractionated on polyacrylamide gels, increased 6-12h after partial hepatectomy and 6h after γ-irridation at 2000 R. The incorporation of orotic acid was particularly increased into the 4.5S, 5S and approx. 10S nRNA fractions. If the irradiation was given after 6h of regeneration and RNA was isolated from the nucleus 12h after hepatectomy then the incorporation of orotic acid into these low-molecular-weight nRNA components was greater than after hepatectomy or irradiation alone.

Noncoordinate regulation of cytoplasmic RNA in compensatory renal hypertrophy

1979 ◽  
Vol 237 (5) ◽  
pp. R360-R365
Author(s):  
A. J. Ouellette ◽  
R. A. Malt
Keyword(s):  
Cell Growth ◽  
Orotic Acid ◽  
Control Mechanism ◽  
Mouse Kidney ◽  
Renal Hypertrophy ◽  
Polyadenylated Rna ◽  
Cytoplasmic Rna ◽  
Compensatory Renal Hypertrophy ◽  
Mrna Half Life

To examine the regulatory role of mRNA in compensatory renal hypertrophy, the accumulation and decay of [3H]orotic acid in poly(A)-containing mRNA in mouse kidney was analyzed after unilateral nephrectomy during the period of maximal rRNA accretion. The distribution of radioactivity between newly synthesized poly(A)-containing and poly(A)-lacing polysomal RNA was altered, but no differences in mRNA half-life were observed in growth compared with effects of sham nephrectomy. Radioactivity in polysomal polyadenylated RNA was diminished by approximately 25% during growth where mice were labeled after nephrectomy, but if mice were labeled 18 h before operation, no difference was noted. Thus, accumulation of newly synthesized poly(A)-containing mRNA relative to RNAs that lack poly(A) is changed early in the course of renal hypertrophy. This noncoordinate regulation may represent a control mechanism effective early in induced cell growth involving mRNAs that lack poly(A).

Differential labeling with orotic acid and uridine in compensatroy renal hypertrophy

1975 ◽  
Vol 229 (4) ◽  
pp. 952-954 ◽  
Author(s):  
JS Ross ◽  
D Malamud ◽  
JA Caulfield ◽  
RA Malt
Keyword(s):  
Orotic Acid ◽  
Compensatory Hypertrophy ◽  
Mouse Kidney ◽  
Proximal Tubules ◽  
Renal Hypertrophy ◽  
Tubular Cells ◽  
Biologic Activity ◽  
Distal Tubules ◽  
Labeled Rna

Using [5-3H]orotic acid and [5-3H]uridine as precursors, we compared the efficiency of labeling and the localization of labeled RNA during compensatory hypertrophy of the mouse kidney. [5-3H]orotic acid in tubules labeled RNA 15 times more intensely that [5-3H]uridine, presumably because of greater incorporation of orotic acid into tubular cells. Of the orotic acid label, 97% was in tubular cells, mostly in the proximal tubules. Only about 80% of the uridine label was in the tubules; the ratio in proximal tubules compared with that in distal tubules was 2:1. No changes in distribution within the nephron were produced during compensatory hypertrophy. [5-3H]uridine should be used as the precursor of generalized labeling is desired, but [5-3H5orotic acid is the better precursor of RNA for many studies of compensatory hypertrophy since it is more efficient and concentrates in the segments of greatest biologic activity.

Increased formation and keeping of ribosomes during dietary recovery of mouse kidney

1986 ◽  
Vol 251 (3) ◽  
pp. E266-E272
Author(s):  
S. Iapalucci-Espinoza ◽  
J. A. Bur ◽  
M. G. Pucciarelli ◽  
R. D. Conde
Keyword(s):  
Ribosomal Proteins ◽  
Orotic Acid ◽  
Mouse Kidney ◽  
Rrna Synthesis ◽  
Synthesis Rate ◽  
Complete Diet ◽  
The Difference ◽  
Polymerase I ◽  
Rrna Degradation

In the kidney of 5-day protein-depleted mice there is a decrease of 23% in the rRNA mass. When these animals are fed with a complete diet, rRNA content is restored to its normal value after 24 h of refeeding. The mechanisms that underlie this phenomenon were studied. It was found that the activity of rRNA polymerase I in the nuclei of kidneys from refed mice showed an increase of about twofold compared with the activity in normal and protein-depleted nuclei. The in vivo incorporation of a large dose (nontrace) of [14C]orotic acid into rRNA was also twofold enhanced in kidneys from refed mice. Ribosome degradation (measured by the disappearance of radioactivity from either ribosomal proteins or rRNA previously labeled by the injection of NaH14CO3 and [14C]orotic acid to the mice, respectively) stopped during the 1st day after refeeding. The estimation of the difference between the rRNA synthesis rate and the net rRNA increase also demonstrated a decrease in the rRNA degradation rate in refed mice.

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