Limited Trypsin-Catalyzed Hydrolysis of Crystalline Human Ceruloplasmin. Partial Characterization of the Digest*

Biochemistry ◽  
1967 ◽  
Vol 6 (10) ◽  
pp. 3185-3197 ◽  
Author(s):  
Charles B. Kasper
Keyword(s):  
Partial Characterization ◽  
Human Ceruloplasmin ◽  
Hydrolysis Of

Isolation and partial characterization of cDNA clone of human ceruloplasmin receptor

2000 ◽  
Vol 129 (5) ◽  
pp. 491-495 ◽  
Author(s):  
L. K. Sasina ◽  
N. V. Tsymbalenko ◽  
N. A. Platonova ◽  
L. V. Puchkova ◽  
O. V. Voronina ◽  
...  
Keyword(s):  
Cdna Clone ◽  
Partial Characterization ◽  
Human Ceruloplasmin ◽  
Ceruloplasmin Receptor

Alcalase hydrolysis of buffalo casein: Preparation and partial characterization of phosphopeptides

2008 ◽  
Vol 136 ◽  
pp. S719 ◽  
Author(s):  
Jingyan Gao ◽  
Guanghua Xie ◽  
Hongbing Chen ◽  
Xin Li
Keyword(s):  
Partial Characterization ◽  
Hydrolysis Of

Enzymatic hydrolysis of agar: purification and characterization of neoagarobiose hydrolase and p-nitrophenyl α-galactoside hydrolases

1975 ◽  
Vol 21 (10) ◽  
pp. 1512-1518 ◽  
Author(s):  
D. F. Day ◽  
W. Yaphe
Keyword(s):  
Enzymatic Hydrolysis ◽  
Hydrolytic Enzymes ◽  
Partial Characterization ◽  
Purification And Characterization ◽  
Hydrolysis Of

The mixture of polysaccharides in the gelling component of agar (agarose) is hydrolyzed to D-galactose and 3,6-anhydro-L-galactose by a series of hydrolytic enzymes obtained from Pseudomonas atlantica. The final degradative step in the pathway of agarose decomposition is the hydrolysis of the α-linkage in the dissaccharide neoagarobiose yielding D-galactose and 3,6-anhydro-L-galactose. Pseudomonas atlantica when grown on agar produces two specific enzymes, p-nitrophenyl α-galactose hydrolase and neoagarobiose hydrolase. The purification and partial characterization of both enzymes are presented.

Isolation and partial characterization of a peptidase with trypsin-like activity from bovine adenohypophysis

1989 ◽  
Vol 54 (8) ◽  
pp. 2276-2286
Author(s):  
Tsezengijn Dash ◽  
Tomislav Barth ◽  
Jiřina Slaninová ◽  
Jana Barthová ◽  
Hana P. Mašková ◽  
...  
Keyword(s):  
Amino Acids ◽  
Molecular Weight ◽  
Partial Characterization ◽  
Chromogenic Substrate ◽  
Fluorogenic Substrate ◽  
Basic Amino Acids ◽  
Optimum Ph ◽  
Reproducible Method ◽  
Hydrolysis Of

A reproducible method has been developed for the isolation of the adenohypophyseal enzyme with a trypsin-like activity. The enzyme is able to hydrolyze Nα-benzoyl-L-arginine-p-nitroanilide, a fluorogenic substrate CBzl-Arg-Arg-β-naphthyl amide and some peptides with one or two accumulated basic amino acids in the chain. The optimum pH for hydrolysis of the chromogenic substrate was within the range 6.0-7.0 (Km = 0.66 mmol l-1), in the case of the fluorogenic substrate the range was between 7.0 and 7.5 (Km = 1.2 μmol l-1). The enzyme is activated by cysteine and dithiothreitol and inhibited by SH-poisons. The molecular weight of the enzyme, determined by means of two independent methods, was approximately 25 kDA.

scholarly journals Isolation and partial characterization of alginate extracted from Sargassum polycystum collected from three habitats in Banten, Indonesia

2019 ◽  
Vol 20 (6) ◽  
Author(s):  
NIKEN DHARMAYANTI ◽  
JATNA SUPRIATNA ◽  
ABINAWANTO ABINAWANTO ◽  
YASMAN YASMAN
Keyword(s):  
Freeze Drying ◽  
Partial Characterization ◽  
Partial Hydrolysis ◽  
Sargassum Polycystum ◽  
Ecological Characteristics ◽  
Mannuronic Acid ◽  
Guluronic Acid ◽  
Hydrolysis Of ◽  
Test Result

Abstract. Dharmayanti N, Supriatna J, Abinawanto, Yasman. 2019. Isolation and partial characterization of Alginate from Sargassum polycystum of Banten, Indonesia. Biodiversitas 20: 1776-1785. The utilization of Sargassum polycystum as an alternative alginate source may reduce the dependence on alginate import as currently alginate demands in Indonesia are 100% supplied from overseas. The purpose of this study was to characterize alginate extracted from Sargassum polycystum obtained from three locations with different ecological characteristics. Isolation of alginate was conducted through partial hydrolysis to separate guluronic acid and mannuronic acid followed by freeze-drying and then the parameters were measured qualitatively and quantitatively using FTIR. The results showed that alginate extracted from Sargassum polycystum collected from Lima Island, Ujung Kulon and Binuangeun were 11.48%, 18.62%, and 5.75%, respectively with the viscosity were 35 cP, 62.50 cP, and 81.33 cP, respectively. The test result of partial hydrolysis of alginate showed that guluronic block (GG) in the alginate polymer of Lima Island, Ujung Kulon and Binuangeun were 67.60%, 59.00%, and 41.40%, respectively. These relate to the nature of the gel formed. The alginate from Lima Island tends to be more rigid and less flexible than that from Ujung Kulon and Binuangeun. The findings of this study suggest that there are differences in the concentration of the components of mannuronate and guluronate of Sargassum polycystum across different locations in Banten, Indonesia.

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