N6-(Δ2-isopentenyl)adenosine. Biosynthesis in transfer ribonucleic acid in vitro

Biochemistry ◽  
1969 ◽  
Vol 8 (11) ◽  
pp. 4361-4371 ◽  
Author(s):  
Larry K. Kline ◽  
Fritz Fittler ◽  
Ross H. Hall
Keyword(s):  
Ribonucleic Acid ◽  
Isopentenyl Adenosine

scholarly journals The Conversion of a Phage-induced Ribonucleic Acid to Deoxyribonucleotides in Vitro

1961 ◽  
Vol 236 (5) ◽  
pp. 1448-1457 ◽  
Author(s):  
Seymour S. Cohen ◽  
Hazel D. Barner ◽  
Janet Lichtenstein
Keyword(s):  
Ribonucleic Acid

Peptide synthesis in bone marrow: insulin and thyroxin effects

1962 ◽  
Vol 203 (4) ◽  
pp. 693-696 ◽  
Author(s):  
Thomas F. Necheles
Keyword(s):  
Bone Marrow ◽  
Nucleic Acid ◽  
Peptide Synthesis ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Optimum Concentration ◽  
The Other ◽  
Anabolic Effect ◽  
Oxygen Buffer

Myeloid marrow was rapidly removed from femurs of fasting young rabbits, sectioned, and incubated in Krebs-bicarbonate-CO2-oxygen buffer with appropriate C14-labeled precursors. All manipulations were designed to preserve the architecture of the tissue. After 1 hr the protein or nucleic acid-adenine was isolated and purified. Insulin, 0.01 U/ml added in vitro, stimulated histidine-2(ring)-C14 incorporation into protein by 26 ± 1.4%; alkali-treated insulin was inactive. Thyroxin elicited a 49.4 ± 2.1% stimulation at an optimum concentration of 10–7 m. Triiodothyronine, but not diiodothyronine, also had a significant effect. Insulin increased incorporation of carbon from adenosine-8-C14 into adenine of ribonucleic acid and deoxyribonucleic acid. Thyroxin, on the other hand, was without consistent effect on this process. Thyroxin stimulated significantly the incorporation of C14 of glycine-2-C14 into adenine. The possibility that part of the anabolic effect of thyroxin on bone marrow may arise from a stimulus to incorporation of precursors into purines is suggested.

Serotonin and Ribonucleic Acid and Collagen Metabolism of Fibroblasts in Vitro

1972 ◽  
Vol 140 (2) ◽  
pp. 599-603 ◽  
Author(s):  
R. J. Boucek ◽  
A. J. Speropoulos ◽  
N. L. Noble
Keyword(s):  
Ribonucleic Acid ◽  
Collagen Metabolism

Translation of satellite tobacco necrosis virus ribonucleic acid. II. Initiation of in vitro translation in procaryotic and eucaryotic systems

Biochemistry ◽  
1972 ◽  
Vol 11 (11) ◽  
pp. 2014-2019 ◽  
Author(s):  
Ronald E. Lundquist ◽  
Jerome M. Lazar ◽  
William H. Klein ◽  
John M. Clark
Keyword(s):  
Ribonucleic Acid ◽  
In Vitro Translation ◽  
Tobacco Necrosis Virus ◽  
Necrosis Virus

A Requirement for β-Mercaptopyruvate in the in Vitro Thiolation of Transfer Ribonucleic Acid*

Biochemistry ◽  
1967 ◽  
Vol 6 (3) ◽  
pp. 855-860 ◽  
Author(s):  
Marie N. Lipsett ◽  
Jane S. Norton ◽  
Alan Peterkofsky
Keyword(s):  
Ribonucleic Acid

scholarly journals ON THE ROLE OF RIBONUCLEIC ACID IN ANIMAL VIRUS SYNTHESIS

1960 ◽  
Vol 111 (3) ◽  
pp. 351-368 ◽  
Author(s):  
Igor Tamm ◽  
Rostom Bablanian
Keyword(s):  
Herpes Simplex ◽  
Vaccinia Virus ◽  
Ribonucleic Acid ◽  
Chorioallantoic Membrane ◽  
Host Cells ◽  
Active Inhibitor ◽  
Herpes Simplex Viruses ◽  
Virus Particles ◽  
Highly Active

Ribonuclease is a highly active inhibitor of vaccinia virus multiplication in vitro in the chorioallantoic membrane removed from embryonated chicken eggs. It is also a highly active inhibitor of pock formation by vaccinia and herpes simplex viruses on the chorioallantoic membrane in vivo. Marked inhibitory effects were obtained with 12.5 µg. of RNase. However, complete inhibition was not obtained with several hundred micrograms of the enzyme. RNase caused no inactivation of the infectivity of vaccinia virus particles but it had a marked inhibitory effect on multiplication of this virus when administered many hours after infection of host cells had occurred. RNase also failed to inactivate the infectivity of herpes simplex virus particles. The results obtained indicate that ribonucleic acid is necessary for the multiplication of two DNA-containing viruses; i.e., vaccinia and herpes simplex.

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