Effect of sequential degradation on the haptenic activity of a tryptic peptide isolated from reduced and alkylated egg-white lysozyme and the haptenic properties of its amino-terminal residues

AbstractThermal modification is an effective method that induces significant expansion of the antimicrobial properties and other valuable properties of chicken egg white lysozyme. In our latest research, a new innovative method of enzyme modification was developed, in which microwave radiation was used as an energy source to process liquid lysozyme concentrate (LLC). After modification, high-quality preparations were obtained. However, long-term storage in a concentrated form initiated various processes that caused darkening over time and could also lead to other significant changes to their structure and, consequently, to their functional properties. This necessitated multidirectional research to explain this phenomenon. This paper presents the results of research aimed at assessing the physicochemical changes in the properties of microwave-modified lysozyme in the form of a liquid concentrate after long-term storage under refrigeration conditions. The assessment also considered the conditions under the acidity of the modifying medium and the duration of the microwave modification. The analysis showed that the values of the basic parameters determining the quality and usefulness of the modified enzyme significantly improved during long-term storage of the preparations. The greatest changes were observed in the preparations modified for the longest time and in the most acidic environment (process time 260 s, pH 2.0), the number of oligomers under these conditions increased by 18% after 12 months of holding, and the surface hydrophobicity increased by as much as 31%. In addition, microbiological tests showed that the preparations of microwave-modified lysozyme had an effect on gram-positive bacteria as well as on gram-negative, and this effect was significantly enhanced after 12 months. The results confirm that LLC modification with microwave radiation is a highly efficient method to prepare high-quality and high utility potential lysozyme. Notably, an interesting and important phenomenon was the observation of the unconventional behaviour of the preparations during their long-term storage, which increased their utility potential significantly.

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Comparison of the Amino Acid Sequence of Bovine α-Lactalbumin and Hens Egg White Lysozyme

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)95873-4 ◽

1967 ◽

Vol 242 (16) ◽

pp. 3747-3748 ◽

Cited By ~ 10

Author(s):

Keith Brew ◽

Thomas C. Vanaman ◽

Robert L. Hill

Keyword(s):

Amino Acid ◽

Amino Acid Sequence ◽

Egg White ◽

Egg White Lysozyme

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Purification and characterization of angiotensin I-converting enzyme inhibitory peptides from enzymatic hydrolysate of hen egg white lysozyme

Food Research International ◽

10.1016/j.foodres.2011.12.005 ◽

2012 ◽

Vol 46 (1) ◽

pp. 127-134 ◽

Cited By ~ 34

Author(s):

Sheng-Qi Rao ◽

Tao Ju ◽

Jun Sun ◽

Yu-Jie Su ◽

Rong-Rong Xu ◽

...

Keyword(s):

Egg White ◽

Hen Egg White Lysozyme ◽

Angiotensin I ◽

Purification And Characterization ◽

Enzymatic Hydrolysate ◽

Inhibitory Peptides ◽

Egg White Lysozyme ◽

Angiotensin I Converting Enzyme ◽

Hen Egg White

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