Transcription of ribonucleic acid by the ribonucleic acid directed deoxyribonucleic acid polymerase of Rous sarcoma virus and deoxyribonucleic acid polymerase I of Escherichia coli

Biochemistry ◽  
1973 ◽  
Vol 12 (3) ◽  
pp. 460-467 ◽  
Author(s):  
J. M. Taylor ◽  
A. J. Faras ◽  
H. E. Varmus ◽  
H. M. Goodman ◽  
W. E. Levinson ◽  
...  
1970 ◽  
Vol 6 (5) ◽  
pp. 589-598 ◽  
Author(s):  
Axel-Claude Garapin ◽  
Jerome P. McDonnell ◽  
Warren Levinson ◽  
Nancy Quintrell ◽  
Lois Fanshier ◽  
...  

1973 ◽  
Vol 11 (5) ◽  
pp. 761-774 ◽  
Author(s):  
J. Thomas Parsons ◽  
John M. Coffin ◽  
Richard K. Haroz ◽  
Peter A. Bromley ◽  
Charles Weissmann

1972 ◽  
Vol 9 (6) ◽  
pp. 891-902 ◽  
Author(s):  
Jo-Ann Leong ◽  
Axel-Claude Garapin ◽  
Nola Jackson ◽  
Lois Fanshier ◽  
Warren Levinson ◽  
...  

1983 ◽  
Vol 3 (10) ◽  
pp. 1746-1758
Author(s):  
B Mermer ◽  
M Malamy ◽  
J M Coffin

Several aspects of Rous sarcoma virus gene expression, including transcription, translation, and protein processing, can occur within Escherichia coli containing cloned viral DNA. The viral long terminal repeat contains a bacterial promoter, and viral sequences at or near the authentic viral initiation codon permit the initiation of translation. These signals can direct the synthesis in E. coli of the viral gag gene precursor Pr76 or, when fused to a portion of the lacZ gene, a gag-beta-galactosidase fusion protein. Pr76 is processed into gag structural proteins in E. coli in a process which is dependent upon the gag product p15. These observations suggest that E. coli can be used for the introduction and analysis of mutations in sequences relevant to viral gene expression.


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