Amino acid sequences of mouse 2.5S nerve growth factor. II. Isolation and characterization of the thermolytic and peptic peptides and the complete covalent structure

Biochemistry ◽  
1973 ◽  
Vol 12 (1) ◽  
pp. 100-115 ◽  
Author(s):  
Ruth Hogue Angeletti ◽  
Mark A. Hermodson ◽  
Ralph A. Bradshaw
Keyword(s):  
Amino Acid ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Amino Acid Sequences ◽  
Nerve Growth ◽  
Isolation And Characterization ◽  
Factor Ii ◽  
Covalent Structure

scholarly journals Isolation and Characterization of a Glycosylated Form of β Nerve Growth Factor in Mouse Submandibular Glands

1989 ◽  
Vol 264 (21) ◽  
pp. 12502-12509 ◽  
Author(s):  
R A Murphy ◽  
V Chlumecky ◽  
L B Smillie ◽  
M Carpenter ◽  
M Nattriss ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Nerve Growth ◽  
Submandibular Glands ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of a nerve growth factor-regulated Fos kinase from PC12 cells.

1994 ◽  
Vol 269 (1) ◽  
pp. 308-318
Author(s):  
L.K. Taylor ◽  
K.D. Swanson ◽  
J. Kerigan ◽  
W. Mobley ◽  
G.E. Landreth
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Pc12 Cells ◽  
Nerve Growth ◽  
Isolation And Characterization

scholarly journals Recombinant production of rabbit β-Nerve Growth Factor and its biological effect on rabbit sperm

2018 ◽  
Author(s):  
Ana Sanchez-Rodriguez ◽  
Paloma Abad ◽  
Maria Arias-Alvarez ◽  
Pilar G. Rebollar ◽  
José M. Bautista ◽  
...  
Keyword(s):  
Amino Acid ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Recombinant Protein ◽  
Dose Response ◽  
Amino Acid Sequences ◽  
Nerve Growth ◽  
Specificity Effect ◽  
Rapid Amplification ◽  
Dose Response Effect

AbstractThe neurotrophin β-Nerve Growth Factor (β-NGF) is flourishing as a protein with important roles in the ovulation induction process in induced-ovulation species but data in rabbits are still inconclusive, probably due to the species-specificity effect of the neurotrophin to trigger the ovulation. Moreover, β-NGF seems to have a role in sperm function. To clarify these functionalities we aimed, in the present research: 1) to newly synthesize a functional recombinant β-NGF from rabbit (rrβ-NGF), 2) to reveal differences in the amino acid sequence of rabbit β-NGF compared to other sequences of induced and spontaneous ovulator species, and 3) to assess the effects of rrβ-NGF on sperm viability and motility. The nucleotide sequence of NGF from rabbit prostate was sequenced by Rapid Amplification of cDNA Ends (RACE) and annotated in GenBank (KX528686). Then, rrβ-NGF was produced in CHO cells and purified by affinity chromatography. Western blot and MALDI-TOF analyses confirmed the correct identity of the recombinant protein. rrβ-NGF functionality was validated in PC12 cells through a successful dose-response effect along 8 days. The comparison of the amino acid sequences of NGF between rabbit and other species suggested some relevant substitutions at its binding site to both the high-(TrkA) and the low-(p75) affinity receptors. The addition of rrβ-NGF in rabbit sperm, in a time- and dose-response study, did not affect its viability but slightly changed some of its motility parameters at the highest concentration used (100 ng/ml). Thus, it can be considered that this new recombinant protein may be used for biotechnological and reproduction assisted techniques in ovulation-induced species.

Isolation and characterization of nerve growth factor from Vipera berus (common viper) venom

1986 ◽  
Vol 83 (3) ◽  
pp. 621-625 ◽  
Author(s):  
Jüri Siigur ◽  
Urmas Arumäe ◽  
Toomas Neuman ◽  
Mari Samel ◽  
Ene Siigur ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Nerve Growth ◽  
Vipera Berus ◽  
Isolation And Characterization

scholarly journals The Isolation and Characterization of Nerve-Growth Factor from the Venom of Vipera russelli

1972 ◽  
Vol 29 (3) ◽  
pp. 417-425 ◽  
Author(s):  
Frederick L. Pearce ◽  
Barbara E. C. Banks ◽  
Derek V. Banthorpe ◽  
Anthony R. Berry ◽  
Hugh ff. S. Davies ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Nerve Growth ◽  
Isolation And Characterization ◽  
Vipera Russelli
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