Amino acid sequences of mouse 2.5S nerve growth factor. I. Isolation and characterization of the soluble tryptic and chymotryptic peptides

Biochemistry ◽  
1973 ◽  
Vol 12 (1) ◽  
pp. 90-100 ◽  
Author(s):  
Ruth Hogue Angeletti ◽  
Delio Mercanti ◽  
Ralph A. Bradshaw
Keyword(s):  
Amino Acid ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Amino Acid Sequences ◽  
Nerve Growth ◽  
Factor I ◽  
Isolation And Characterization ◽  
Growth Factor I

Linkage relationships of the gene for the β subunit of nerve growth factor (NGFB) with other chromosome 1 marker loci

1985 ◽  
Vol 39 (2) ◽  
pp. 158-160 ◽  
Author(s):  
J.K. Darby ◽  
J.R. Kidd ◽  
A.J. Pakstis ◽  
R.S. Sparkes ◽  
H.M. Cann ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Beta Subunit ◽  
Chromosome 1 ◽  
Nerve Growth ◽  
Factor I ◽  
Marker Loci ◽  
Growth Factor I ◽  
Linkage Relationships

scholarly journals Isolation and Characterization of a Glycosylated Form of β Nerve Growth Factor in Mouse Submandibular Glands

1989 ◽  
Vol 264 (21) ◽  
pp. 12502-12509 ◽  
Author(s):  
R A Murphy ◽  
V Chlumecky ◽  
L B Smillie ◽  
M Carpenter ◽  
M Nattriss ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Nerve Growth ◽  
Submandibular Glands ◽  
Isolation And Characterization

Comparison of 7S nerve growth factor and nerve growth factor I from mouse submandibular glands

Biochemistry ◽  
1986 ◽  
Vol 25 (4) ◽  
pp. 754-760 ◽  
Author(s):  
Nicholas G. Guerina ◽  
Nicholas J. Pantazis ◽  
Kerry Siminoski ◽  
Jacqueline K. Anderson ◽  
Mary McCarthy ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Nerve Growth ◽  
Submandibular Glands ◽  
Factor I ◽  
Growth Factor I

scholarly journals Isolation and characterization of a nerve growth factor-regulated Fos kinase from PC12 cells.

1994 ◽  
Vol 269 (1) ◽  
pp. 308-318
Author(s):  
L.K. Taylor ◽  
K.D. Swanson ◽  
J. Kerigan ◽  
W. Mobley ◽  
G.E. Landreth
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Pc12 Cells ◽  
Nerve Growth ◽  
Isolation And Characterization

scholarly journals Recombinant production of rabbit β-Nerve Growth Factor and its biological effect on rabbit sperm

2018 ◽  
Author(s):  
Ana Sanchez-Rodriguez ◽  
Paloma Abad ◽  
Maria Arias-Alvarez ◽  
Pilar G. Rebollar ◽  
José M. Bautista ◽  
...  
Keyword(s):  
Amino Acid ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Recombinant Protein ◽  
Dose Response ◽  
Amino Acid Sequences ◽  
Nerve Growth ◽  
Specificity Effect ◽  
Rapid Amplification ◽  
Dose Response Effect

AbstractThe neurotrophin β-Nerve Growth Factor (β-NGF) is flourishing as a protein with important roles in the ovulation induction process in induced-ovulation species but data in rabbits are still inconclusive, probably due to the species-specificity effect of the neurotrophin to trigger the ovulation. Moreover, β-NGF seems to have a role in sperm function. To clarify these functionalities we aimed, in the present research: 1) to newly synthesize a functional recombinant β-NGF from rabbit (rrβ-NGF), 2) to reveal differences in the amino acid sequence of rabbit β-NGF compared to other sequences of induced and spontaneous ovulator species, and 3) to assess the effects of rrβ-NGF on sperm viability and motility. The nucleotide sequence of NGF from rabbit prostate was sequenced by Rapid Amplification of cDNA Ends (RACE) and annotated in GenBank (KX528686). Then, rrβ-NGF was produced in CHO cells and purified by affinity chromatography. Western blot and MALDI-TOF analyses confirmed the correct identity of the recombinant protein. rrβ-NGF functionality was validated in PC12 cells through a successful dose-response effect along 8 days. The comparison of the amino acid sequences of NGF between rabbit and other species suggested some relevant substitutions at its binding site to both the high-(TrkA) and the low-(p75) affinity receptors. The addition of rrβ-NGF in rabbit sperm, in a time- and dose-response study, did not affect its viability but slightly changed some of its motility parameters at the highest concentration used (100 ng/ml). Thus, it can be considered that this new recombinant protein may be used for biotechnological and reproduction assisted techniques in ovulation-induced species.

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