Translation of satellite tobacco necrosis virus ribonucleic acid by an in vitro system from wheat germ

Biochemistry ◽  
1976 ◽  
Vol 15 (22) ◽  
pp. 4943-4950 ◽  
Author(s):  
David W. Leung ◽  
Carl W. Gilbert ◽  
Robert E. Smith ◽  
Nancy L. Sasavage ◽  
John M. Clark
Keyword(s):  
Ribonucleic Acid ◽  
Wheat Germ ◽  
Tobacco Necrosis Virus ◽  
Necrosis Virus ◽  
In Vitro System

Translation of satellite tobacco necrosis virus ribonucleic acid. II. Initiation of in vitro translation in procaryotic and eucaryotic systems

Biochemistry ◽  
1972 ◽  
Vol 11 (11) ◽  
pp. 2014-2019 ◽  
Author(s):  
Ronald E. Lundquist ◽  
Jerome M. Lazar ◽  
William H. Klein ◽  
John M. Clark
Keyword(s):  
Ribonucleic Acid ◽  
In Vitro Translation ◽  
Tobacco Necrosis Virus ◽  
Necrosis Virus

Translation of satellite tobacco necrosis virus ribonucleic acid. I. Characterization of in vitro procaryotic and eucaryotic translation products

Biochemistry ◽  
1972 ◽  
Vol 11 (11) ◽  
pp. 2009-2014 ◽  
Author(s):  
William H. Klein ◽  
Chris Nolan ◽  
Jerome M. Lazar ◽  
John M. Clark
Keyword(s):  
Ribonucleic Acid ◽  
Tobacco Necrosis Virus ◽  
Necrosis Virus

Protection of satellite tobacco necrosis virus ribonucleic acid by wheat germ 40S and 80S ribosomes

Biochemistry ◽  
1980 ◽  
Vol 19 (10) ◽  
pp. 2276-2283 ◽  
Author(s):  
Karen S. Browning ◽  
David W. Leung ◽  
John M. Clark
Keyword(s):  
Ribonucleic Acid ◽  
Wheat Germ ◽  
Tobacco Necrosis Virus ◽  
Necrosis Virus

scholarly journals Competitive mRNA translation in an in vitro system from wheat germ.

1979 ◽  
Vol 254 (17) ◽  
pp. 8245-8249
Author(s):  
D. Herson ◽  
A. Schmidt ◽  
S. Seal ◽  
A. Marcus ◽  
L. van Vloten-Doting
Keyword(s):  
Wheat Germ ◽  
Mrna Translation ◽  
In Vitro System

scholarly journals The 3' untranslated region of satellite tobacco necrosis virus RNA stimulates translation in vitro.

1993 ◽  
Vol 13 (6) ◽  
pp. 3340-3349 ◽  
Author(s):  
X Danthinne ◽  
J Seurinck ◽  
F Meulewaeter ◽  
M Van Montagu ◽  
M Cornelissen
Keyword(s):  
Tobacco Necrosis Virus ◽  
Translation System ◽  
Coding Region ◽  
Necrosis Virus ◽  
Untranslated Sequence ◽  
Virus Rna ◽  
Free Translation ◽  
Order Of Magnitude ◽  
A Cell

The RNA of satellite tobacco necrosis virus (STNV) is a monocistronic messenger that lacks both a 5' cap structure and a 3' poly(A) tail. We show that in a cell-free translation system derived from wheat germ, STNV RNA lacking the 600-nucleotide trailer is translated an order of magnitude less efficiently than full-size RNA. Deletion analyses positioned the translational enhancer domain (TED) within a conserved hairpin structure immediately downstream from the coat protein cistron. TED enhances translation when fused to a heterologous mRNA, but the level of enhancement depends on the nature of the 5' untranslated sequence and is maximal in combination with the STNV leader. The STNV leader and TED have two regions of complementarity. One of the complementary regions in TED resembles picornavirus box A, which is involved in cap-independent translation but which is located upstream of the coding region.

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