Use of ethidium bromide fluorescence enhancement to detect duplex DNA and DNA bacteriophages during zone sedimentation in sucrose gradients: molecular weight of DNA as a function of sedimentation rate

Biochemistry ◽  
1978 ◽  
Vol 17 (7) ◽  
pp. 1166-1170 ◽  
Author(s):  
Philip Serwer ◽  
Paul R. Graef ◽  
Preston N. Garrison
Keyword(s):  
Molecular Weight ◽  
Ethidium Bromide ◽  
Sedimentation Rate ◽  
Fluorescence Enhancement ◽  
Duplex Dna ◽  
Molecular Weight Of Dna

scholarly journals Sedimentation Rate as a Measure of Molecular Weight of DNA

1963 ◽  
Vol 3 (4) ◽  
pp. 309-321 ◽  
Author(s):  
Elizabeth Burgi ◽  
A.D. Hershey
Keyword(s):  
Molecular Weight ◽  
Sedimentation Rate ◽  
Molecular Weight Of Dna

Key factors in FTIR spectroscopic analysis of DNA: the sampling technique, pretreatment temperature and sample concentration

2018 ◽  
Vol 10 (21) ◽  
pp. 2436-2443 ◽  
Author(s):  
Yahong Han ◽  
Lujia Han ◽  
Yumei Yao ◽  
Yanfei Li ◽  
Xian Liu
Keyword(s):  
Molecular Weight ◽  
Fourier Transform ◽  
Ftir Spectroscopy ◽  
Deoxyribonucleic Acid ◽  
Spectroscopic Analysis ◽  
Sampling Technique ◽  
Key Factors ◽  
Sample Concentration ◽  
Pretreatment Temperature ◽  
Molecular Weight Of Dna

Fourier transform infrared (FTIR) spectroscopy has been considered as a powerful tool for analysing the characteristics of deoxyribonucleic acid (DNA) regardless of physical states, sample amounts and the molecular weight of DNA.

Reinigung und Charakterisierung zweier Proteine des Adenovirus Typ 2

1965 ◽  
Vol 20 (8) ◽  
pp. 747-752 ◽  
Author(s):  
Kurt Köhler
Keyword(s):  
Molecular Weight ◽  
Sedimentation Rate ◽  
Adenovirus Type ◽  
Kb Cells ◽  
C Protein ◽  
Different Types ◽  
Adenovirus Type 2

Two soluble antigens produced in KB-cells after infection with Adenovirus type 2 were identified with constituents of the virion itself. Purification and concentration of the two proteins (named A- and C-Protein according to PEREIRA) is described. The sedimentation rate of the A-Protein is 11.5 S, which gives a molecular weight of 310 000, and the corresponding data for the C-Protein are 5.5 S and 81 000 molecular weight. The A-Proteins of different types, 2, 3 and 5 are physically similar, but serologically composed of parts with similar and parts with type-specific differences.

Bifunctional Photobinding of Psoralen to Single Stranded Nucleic Acids

1973 ◽  
Vol 28 (7-8) ◽  
pp. 370-375 ◽  
Author(s):  
S Marciani ◽  
M. Terbojevich ◽  
F Dall 'Acqua ◽  
G. Rodighiero
Keyword(s):  
Molecular Weight ◽  
Light Scattering ◽  
Nucleic Acids ◽  
Dilute Solutions ◽  
Base Pairs ◽  
Bacterial Dna ◽  
Disordered Structure ◽  
Concentrated Solution ◽  
Reduced Rate ◽  
Molecular Weight Of Dna

Abstract As psoralen and other furocoumarin derivatives, intercalated between two base pairs of native DNA, under irradiation at 365 nm form inter-strand cross-linkings as a consequence of bifunctional addition, the writers have investigated the ability of psoralen to give such bifunctional photo­ additions, too, with nucleic acids with disordered or partilly disordered structure (denatured DNA and r-RNA). On the basis of fluorimetric, light-scattering, viscosimetric measurements and of the renaturation ability of denatured bacterial DNA, certain results have been obtained. In addition to monofunctional photoadditions, psoralen can give bifunctional binding by irradiation at 365 nm both with denatured DNA and with r-RNA. However, when irradiation of denatured DNA in the presence of psoralen was performed in a concentrated solution (0.4%), the formation of bifunctional additions between two different strands was demonstrated by the increase (50%) of molecular weight of denatured DNA. However, when irradiation of denatured DNA was performed in more dilute solutions (0.1%), the bifunctional photoaddition of psoralen took place producing only bi­ functional additions in the same strand, very probably with the formation of loops, as has been shown by the absence of increase of molecular weight of DNA and by the more restricted structure assumed by the macromolecule, revealed by the light-scattering and viscosimetric measurements. The formation of these bifunctional additions was confirmed by the reduced rate of renaturation shown by denatured bacterial DNA after irradiation in the presence of psoralen. In the case of r-RNA, psoralen, when irradiated can form bifunctional additions only in the same strand.

A Fluorescence Assay for DNA with Covalently Linked Complementary Sequences

1972 ◽  
Vol 50 (2) ◽  
pp. 210-216 ◽  
Author(s):  
A. Richard Morgan ◽  
Verner Paetkau
Keyword(s):  
Ethidium Bromide ◽  
Fluorescence Enhancement ◽  
Nucleation Site ◽  
Heat Denaturation ◽  
Heating And Cooling ◽  
Physical Measurements ◽  
Complementary Sequences ◽  
Naturally Occurring ◽  
Nucleation Sites ◽  
Covalently Linked

An assay which measures polydeoxyribonucleates containing covalently linked complementary sequences is described. It is based on the fluorescence enhancement of ethidium bromide bound to bihelical polydeoxyribonucleates. This dependence of fluorescence enhancement upon bihelicity has been further documented. Synthetic, defined polymers, such as d(A)n∙d(T)n, d(AT)n∙d(AT)n, d(TC)n∙d(GA)n, and d(TTG)n∙d(CAA)n, have been studied, as well as naturally occurring DNAs. At moderate ionic strengths, the synthetic polymers will re-anneal following heat denaturation. Their facility to renature is due to the self-complementarity between strands throughout their lengths. This facility is lost at very low ionic strengths (Γ/2 < 0.003 M), where ionic repulsion prevents the formation of "nucleation" sites for annealing. However, a covalent linkage between strands provides a nucleation site for renaturation. The fluorescence seen with ethidium bromide after heating and cooling such polymers is then a measure of DNA which has covalently linked complementary sequences. By this assay, natural DNAs appear to contain short, relatively unstable bihelical regions following heat denaturation and cooling, in agreement with models based on other physical measurements.

Strand breakage and decreased molecular weight of DNA induced by specific metal compounds

1982 ◽  
Vol 3 (6) ◽  
pp. 657-662 ◽  
Author(s):  
Steven H. Robison ◽  
Orazio Cantoni ◽  
Max Costa
Keyword(s):  
Molecular Weight ◽  
Metal Compounds ◽  
Strand Breakage ◽  
Molecular Weight Of Dna ◽  
Specific Metal

APPLICATION OF THE ARCHIBALD ULTRACENTRIFUGAL PROCEDURE TO LYSOZYME AND APURINIC ACID: EVALUATION USING A MECHANICAL INTEGRATOR

1956 ◽  
Vol 34 (3) ◽  
pp. 364-370 ◽  
Author(s):  
David B. Smith ◽  
G. C. Wood ◽  
P. A. Charlwood
Keyword(s):  
Molecular Weight ◽  
Sedimentation Rate ◽  
Computational Work ◽  
Transient Data ◽  
Equilibrium Ultracentrifugation

The method outlined by Archibald for handling transient data in equilibrium ultracentrifugation yields a molecular weight for lysozyme that conforms to those given by other methods. A method for applying the Archibald procedure to apurinic acid which is polydisperse and has a concentration dependent sedimentation rate is outlined. The use of a mechanical integrator for reducing the computational work is described.

Isolation of closed-circular duplex DNA by chromatography on hydroxyapatite in the presence of ethidium bromide

1975 ◽  
Vol 67 (2) ◽  
pp. 372-383 ◽  
Author(s):  
Werner Pakroppa ◽  
Werner Goebel ◽  
Werner Müller
Keyword(s):  
Ethidium Bromide ◽  
Duplex Dna
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