Kinetics of Sendai virus envelope fusion with erythrocyte membranes and virus-induced hemolysis

Biochemistry ◽  
1979 ◽  
Vol 18 (23) ◽  
pp. 5088-5095 ◽  
Author(s):  
Douglas S. Lyles ◽  
Frank R. Landsberger
Keyword(s):  
Sendai Virus ◽  
Erythrocyte Membranes ◽  
Virus Envelope ◽  
Kinetics Of

Characterization of the fusogenic properties of Sendai virus: kinetics of fusion with erythrocyte membranes

Biochemistry ◽  
1985 ◽  
Vol 24 (18) ◽  
pp. 4739-4745 ◽  
Author(s):  
Dick Hoekstra ◽  
Karin Klappe ◽  
Tiny De Boer ◽  
Jan Wilschut
Keyword(s):  
Sendai Virus ◽  
Erythrocyte Membranes ◽  
Kinetics Of

Use of a fluorescence assay to monitor the kinetics of fusion between erythrocyte ghosts, as induced by sendai virus

1986 ◽  
Vol 6 (11) ◽  
pp. 953-960 ◽  
Author(s):  
Dick Hoekstra ◽  
Karin Klappe
Keyword(s):  
Rhodamine B ◽  
Experimental Approach ◽  
Sendai Virus ◽  
Fluorescence Assay ◽  
Fusion Process ◽  
Erythrocyte Membranes ◽  
Erythrocyte Ghosts ◽  
Wide Range ◽  
Kinetics Of ◽  
Insight Into

The kinetics of the fusion process between erythrocyte ghosts, as induced by Sendal virus, were readily revealed by a simple fluorescence procedure previously employed to characterize the fusion of viruses with biological membranes. The method relies on the relief of fluorescence selfquenching of the membrane-inserted probe octadecyl Rhodamine B chloride (R18) as occurs when labeled membranes fuse with unlabeled counterparts. The kinetics of R18 insertion into ghost membranes, the non-exchangeable properties of the fluorophore and the kinetics, and some characteristics of Sendai virus-induced fusion of ghosts, are described. We propose that the experimental approach may be particularly advantageous to obtain insight into the efficiency and mechanism of a wide range of fusogens, capable of inducing fusion of erythrocyte membranes.

scholarly journals Distinctive Kinetics of the Antibody-Forming Cell Response to Sendai Virus Infection of Mice in Different Anatomical Compartments

Virology ◽  
1995 ◽  
Vol 207 (1) ◽  
pp. 287-291 ◽  
Author(s):  
Mark Sangster ◽  
Lisa Hyland ◽  
Robert Sealy ◽  
Christopher Coleclough
Keyword(s):  
Virus Infection ◽  
Cell Response ◽  
Sendai Virus ◽  
Kinetics Of

Studies of membrane fusion. VI. Mechanism of the membrane fusion and cell swelling stages of Sendai virus-mediated cell fusion

1980 ◽  
Vol 43 (1) ◽  
pp. 103-118
Author(s):  
S. Knutton
Keyword(s):  
Membrane Fusion ◽  
Cell Fusion ◽  
Sendai Virus ◽  
Freeze Fracture ◽  
Viral Envelope ◽  
Cell Swelling ◽  
Fusion Event ◽  
Virus Envelope ◽  
Membrane Rupture ◽  
Membrane Tubules

The membrane fusion and cell swelling stages of Sendai virus-mediated cell-cell fusion have been studied by thin-section and freeze-fracture electron microscopy. Sites of membrane fusion have been detected in human erythrocytes arrested at the membrane fusion stage of cell fusion and in virtually all cases a fused viral envelope or envelope components has been identified thus providing further direct evidence that cell-viral envelope-cell bridge formation is the membrane fusion event in Sendai virus-induced cell fusion. Radial expansion of a single virus bridge connecting 2 cells is sufficient to produce a fused cell. Membrane redistribution which occurs during this cell swelling stage of the fusion process is often accompanied by the formation of a system of membrane tubules in the plane of expansion of the virus bridge. The tubules originate from points of fusion between the bridging virus envelope and the erythrocyte membrane and also expand radially as cells swell. Ultimately membrane rupture occurs and the tubules appear to break down as small vesicles. When previously observed in cross-sectioned cells these membrane tubules were interpreted as sites of direct membrane fusion. The present study indicates that this interpretation is incorrect and shows that the tubules are generated subsequent to membrane fusion when 2 cells connected by a virus bridge are induced to swell. A mechanism to explain the formation of this system of membrane tubules is proposed.

scholarly journals Gene Therapy of Melanoma Using Inactivated Sendai Virus Envelope Vector (HVJ-E) with Intrinsic Anti-Tumor Activities

10.5772/17492 ◽  
2011 ◽  
Author(s):  
Yasufumi Kaneda ◽  
Eiji Kiyohara ◽  
Toshihiro Nakajima ◽  
Toshimitsu Itai
Keyword(s):  
Gene Therapy ◽  
Sendai Virus ◽  
Virus Envelope

Effect of membrane potential of fusion of sendai virus envelope with chick erythrocyte membrane and on virus-induced hemolysis

1988 ◽  
Vol 106 (6) ◽  
pp. 1748-1751
Author(s):  
V. A. Slepushkin ◽  
V. M. Chumakov ◽  
A. �. Kalmanson ◽  
S. M. Marchenko ◽  
A. G. Bukrinskaya
Keyword(s):  
Membrane Potential ◽  
Erythrocyte Membrane ◽  
Sendai Virus ◽  
Virus Envelope

Dephosphorylation of human erythrocyte membranes induced by Sendai virus

Biochemistry ◽  
1977 ◽  
Vol 16 (17) ◽  
pp. 3903-3909 ◽  
Author(s):  
A. Loyter ◽  
R. Ben-Zaquen ◽  
R. Marash ◽  
Y. Milner
Keyword(s):  
Human Erythrocyte ◽  
Sendai Virus ◽  
Erythrocyte Membranes ◽  
Human Erythrocyte Membranes
Sign in / Sign up

Export Citation Format

Share Document