Enzymic synthesis of deoxyribonucleic acid by the avian retrovirus reverse transcriptase in vitro: optimum conditions required for transcription of large ribonucleic acid templates

Biochemistry ◽  
1980 ◽  
Vol 19 (3) ◽  
pp. 513-518 ◽  
Author(s):  
Ernest F. Retzel ◽  
Marc S. Collett ◽  
Anthony J. Faras
Keyword(s):  
Reverse Transcriptase ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Optimum Conditions ◽  
Enzymic Synthesis

Peptide synthesis in bone marrow: insulin and thyroxin effects

1962 ◽  
Vol 203 (4) ◽  
pp. 693-696 ◽  
Author(s):  
Thomas F. Necheles
Keyword(s):  
Bone Marrow ◽  
Nucleic Acid ◽  
Peptide Synthesis ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Optimum Concentration ◽  
The Other ◽  
Anabolic Effect ◽  
Oxygen Buffer

Myeloid marrow was rapidly removed from femurs of fasting young rabbits, sectioned, and incubated in Krebs-bicarbonate-CO2-oxygen buffer with appropriate C14-labeled precursors. All manipulations were designed to preserve the architecture of the tissue. After 1 hr the protein or nucleic acid-adenine was isolated and purified. Insulin, 0.01 U/ml added in vitro, stimulated histidine-2(ring)-C14 incorporation into protein by 26 ± 1.4%; alkali-treated insulin was inactive. Thyroxin elicited a 49.4 ± 2.1% stimulation at an optimum concentration of 10–7 m. Triiodothyronine, but not diiodothyronine, also had a significant effect. Insulin increased incorporation of carbon from adenosine-8-C14 into adenine of ribonucleic acid and deoxyribonucleic acid. Thyroxin, on the other hand, was without consistent effect on this process. Thyroxin stimulated significantly the incorporation of C14 of glycine-2-C14 into adenine. The possibility that part of the anabolic effect of thyroxin on bone marrow may arise from a stimulus to incorporation of precursors into purines is suggested.

Template Integrity and the Activity of Eukaryotic Deoxyribonucleic Acid-Dependent Ribonucleic Acid Polymerase in vitro

1973 ◽  
Vol 1 (3) ◽  
pp. 650-655 ◽  
Author(s):  
PETER H. W. BUTTERWORTH ◽  
SARAH JANE FLINT ◽  
C. JAMES CHESTERTON
Keyword(s):  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid

In vitro Examination of the Ribonucleic Acid–Deoxyribonucleic Acid–Deoxyribonuclease System in Mammals

Nature ◽  
1962 ◽  
Vol 194 (4834) ◽  
pp. 1176-1177 ◽  
Author(s):  
J. Y. LE TALAER ◽  
B. FESTY ◽  
J. B. LE PECQ ◽  
C. PAOLETTI
Keyword(s):  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Acid Deoxyribonuclease ◽  
In Vitro Examination

scholarly journals Nucleic Acids and the Biosynthesis of Collagen

1957 ◽  
Vol 10 (3) ◽  
pp. 398 ◽  
Author(s):  
PH Springell
Keyword(s):  
Nucleic Acids ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Protein Fractions ◽  
Marked Inhibition ◽  
In Vitro Incubation ◽  
Skin Protein ◽  
Pre Treatment ◽  
Salt Fractionation

The study of skin protein fractions obtained by salt fractionation indicates that collagen is associated to a greater extent with the ribonucleoprotein fraction than with the deoxyribonucleoprotein fraction. The gelatin derived from the ribonucleoprotein fraction following in vitro incubation of foetal lambskin in the presence of glycine-2-14C was appreciably radioactive. Pre-treatment of the skin with crystalline ribonuclease resulted in a marked inhibition of glycine incorporation into total gelatin. whereas crystalline deoxyribonuclease had little effect. It is therefore concluded that ribonucleic acid rather than deoxyribonucleic acid is associated with the biosynthesis of collagen.

scholarly journals QUANTITATIVE STUDIES OF PHYTOHEMAGGLUTININ-INDUCED DNA AND RNA SYNTHESIS IN NORMAL AND AGAMMAGLOBULINEMIC LEUKOCYTES

1967 ◽  
Vol 125 (5) ◽  
pp. 863-872 ◽  
Author(s):  
Douglass C. Tormey ◽  
Roberta Kamin ◽  
H. Hugh Fudenberg
Keyword(s):  
Ribonucleic Acid ◽  
Rna Synthesis ◽  
Deoxyribonucleic Acid ◽  
In Vitro Synthesis ◽  
Dna And Rna ◽  
Quantitative Studies ◽  
Precursor Synthesis ◽  
Dna And Rna Synthesis

Leukocytes from nine patients with acquired agammaglobulinemia were studied in vitro. Synthesis of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) induced by phytohemagglutinin was measured by determination of the degree of incorporation of labeled precursor. Synthesis of both DNA and RNA was decreased in the agammaglobulinemic cells. The presence of an inhibitor in the patients' sera could not be demonstrated. These results suggest that the basic defect in agammaglobulinemia is cellular rather than humoral.

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