Fluorescence lifetime and time-resolved polarization anisotropy studies of acyl chain order and dynamics in lipid bilayers

Biochemistry ◽  
1981 ◽  
Vol 20 (10) ◽  
pp. 2800-2810 ◽  
Author(s):  
Paul K. Wolber ◽  
Bruce S. Hudson
Keyword(s):  
Lipid Bilayers ◽  
Fluorescence Lifetime ◽  
Acyl Chain ◽  
Polarization Anisotropy ◽  
Time Resolved ◽  
Chain Order

scholarly journals Synaptotagmin 1 Modulates Lipid Acyl Chain Order in Lipid Bilayers by Demixing Phosphatidylserine

2011 ◽  
Vol 286 (28) ◽  
pp. 25291-25300 ◽  
Author(s):  
Alex L. Lai ◽  
Lukas K. Tamm ◽  
Jeffrey F. Ellena ◽  
David S. Cafiso
Keyword(s):  
Lipid Bilayers ◽  
Acyl Chain ◽  
Chain Order ◽  
Synaptotagmin 1

scholarly journals Subcellular localization-dependent changes in EGFP fluorescence lifetime measured by time-resolved flow cytometry

2013 ◽  
Vol 4 (8) ◽  
pp. 1390 ◽  
Author(s):  
Ali Vaziri Gohar ◽  
Ruofan Cao ◽  
Patrick Jenkins ◽  
Wenyan Li ◽  
Jessica P. Houston ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Subcellular Localization ◽  
Fluorescence Lifetime ◽  
Time Resolved ◽  
Egfp Fluorescence

scholarly journals Thulium-Doped Silica Fibers with Enhanced Fluorescence Lifetime and Their Application in Ultrafast Fiber Lasers

Fibers ◽  
2018 ◽  
Vol 6 (3) ◽  
pp. 66 ◽  
Author(s):  
Jakub Cajzl ◽  
Pavel Peterka ◽  
Maciej Kowalczyk ◽  
Jan Tarka ◽  
Grzegorz Sobon ◽  
...  
Keyword(s):  
Optical Fibers ◽  
Fluorescence Lifetime ◽  
Energy Levels ◽  
Local Environment ◽  
Alumina Nanoparticles ◽  
Fluorescence Lifetimes ◽  
Transfer Coefficients ◽  
Relaxation Energy ◽  
Time Resolved ◽  
Conventional Solution

In this work we report on the thulium-doped silica-based optical fibers with increased fluorescence lifetime of the 3F4 level thanks to the modification of the local environment of thulium ions by high content of alumina. The determination of the cross-relaxation energy-transfer coefficients from the measurements of the fluorescence lifetimes of the 3F4 and 3H4 energy levels of Tm3+ ions in the experimentally prepared optical fiber is provided as well. Preforms of optical fibers were prepared either by conventional solution-doping of Tm3+ and Al3+ ions or by dispersion-doping of Tm3+ ions with alumina nanoparticles. Optical fibers were characterized by means of Tm, Al, and Ge concentrations, refractive index profiles, optical spectral absorption and luminescence, and by time-resolved fluorescence spectroscopy. Highly aluminium-codoped thulium silicate optical fibers exhibited fluorescence lifetimes of over ~500 μs with maximum value of 756 μs, which means a fluorescence lifetime enhancement when compared to the thulium-doped fibers reported elsewhere. We show an application of the thulium-doped fiber in a compact all-fiber ring laser that is passively mode-locked by using graphene-based saturable absorber. The output pulsewidth and repetition rate were 905 fs and 32.67 MHz, respectively.

scholarly journals The biochemical resolving power of fluorescence lifetime imaging

2021 ◽  
Author(s):  
Andrew L. Trinh ◽  
Alessandro Esposito
Keyword(s):  
Fluorescence Lifetime ◽  
Resolving Power ◽  
Fluorescence Lifetime Imaging ◽  
Time Resolved ◽  
Instrument Response Function ◽  
Lifetime Imaging ◽  
Use Of Time ◽  
Single Living Cells ◽  
Microscopy Techniques

AbstractA deeper understanding of spatial resolution in microscopy fostered a technological revolution that is now permitting us to investigate the structure of the cell with nanometer resolution. Although fluorescence microscopy techniques enable scientists to investigate both the structure and biochemistry of the cell, the biochemical resolving power of a microscope is a physical quantity that is not well-defined or studied. To overcome this limitation, we carried out a theoretical investigation of the biochemical resolving power in fluorescence lifetime imaging microscopy, one of the most effective tools to investigate biochemistry in single living cells. With the theoretical analysis of information theory and Monte Carlo simulations, we describe how the ‘biochemical resolving power’ in time-resolved sensing depends on instrument specifications. We unravel common misunderstandings on the role of the instrument response function and provide theoretical insights that have significant practical implications in the design and use of time-resolved instrumentation.

Fluorescence lifetime-based sensing of polymersome leakage

2017 ◽  
Vol 16 (2) ◽  
pp. 155-158 ◽  
Author(s):  
Stephan Wang ◽  
Zhong-Ren Chen
Keyword(s):  
Fluorescence Lifetime ◽  
Polymer Degradation ◽  
Fluorescence Technique ◽  
Fluorescence Lifetimes ◽  
Time Resolved Fluorescence ◽  
Time Resolved

The time-resolved fluorescence technique enables us to differentiate between polymer degradation and vesicle leakage by employing fluorescence lifetimes and their amplitudes.

Spectroscopic evidence for hydration and dehydration of lipid bilayers upon interaction with metal ions: a new physical insight

2018 ◽  
Vol 20 (21) ◽  
pp. 14796-14807 ◽  
Author(s):  
Soumya Kanti De ◽  
Nishu Kanwa ◽  
Mirajuddin Ahamed ◽  
Anjan Chakraborty
Keyword(s):  
Steady State ◽  
Metal Ions ◽  
Lipid Bilayers ◽  
Phospholipid Bilayers ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Membrane Probe ◽  
Physical Insight ◽  
Chain Lengths ◽  
Hydration And Dehydration

In this manuscript, we investigate the interactions of different metal ions with zwitterionic phospholipid bilayers of different chain lengths using the well-known membrane probe PRODAN and steady state and time resolved fluorescence spectroscopy.

Structure of Escherichia coli membranes. Fatty acyl chain order parameters of inner and outer membranes and derived liposomes

Biochemistry ◽  
1980 ◽  
Vol 19 (8) ◽  
pp. 1638-1643 ◽  
Author(s):  
Hans Ulrich Gally ◽  
Gerd Pluschke ◽  
Peter Overath ◽  
Joachim Seelig
Keyword(s):  
Escherichia Coli ◽  
Acyl Chain ◽  
Fatty Acyl ◽  
Order Parameters ◽  
Fatty Acyl Chain ◽  
Outer Membranes ◽  
Chain Order
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