scholarly journals Thulium-Doped Silica Fibers with Enhanced Fluorescence Lifetime and Their Application in Ultrafast Fiber Lasers

Fibers ◽  
2018 ◽  
Vol 6 (3) ◽  
pp. 66 ◽  
Author(s):  
Jakub Cajzl ◽  
Pavel Peterka ◽  
Maciej Kowalczyk ◽  
Jan Tarka ◽  
Grzegorz Sobon ◽  
...  
Keyword(s):  
Optical Fibers ◽  
Fluorescence Lifetime ◽  
Energy Levels ◽  
Local Environment ◽  
Alumina Nanoparticles ◽  
Fluorescence Lifetimes ◽  
Transfer Coefficients ◽  
Relaxation Energy ◽  
Time Resolved ◽  
Conventional Solution

In this work we report on the thulium-doped silica-based optical fibers with increased fluorescence lifetime of the 3F4 level thanks to the modification of the local environment of thulium ions by high content of alumina. The determination of the cross-relaxation energy-transfer coefficients from the measurements of the fluorescence lifetimes of the 3F4 and 3H4 energy levels of Tm3+ ions in the experimentally prepared optical fiber is provided as well. Preforms of optical fibers were prepared either by conventional solution-doping of Tm3+ and Al3+ ions or by dispersion-doping of Tm3+ ions with alumina nanoparticles. Optical fibers were characterized by means of Tm, Al, and Ge concentrations, refractive index profiles, optical spectral absorption and luminescence, and by time-resolved fluorescence spectroscopy. Highly aluminium-codoped thulium silicate optical fibers exhibited fluorescence lifetimes of over ~500 μs with maximum value of 756 μs, which means a fluorescence lifetime enhancement when compared to the thulium-doped fibers reported elsewhere. We show an application of the thulium-doped fiber in a compact all-fiber ring laser that is passively mode-locked by using graphene-based saturable absorber. The output pulsewidth and repetition rate were 905 fs and 32.67 MHz, respectively.

Fluorescence lifetime-based sensing of polymersome leakage

2017 ◽  
Vol 16 (2) ◽  
pp. 155-158 ◽  
Author(s):  
Stephan Wang ◽  
Zhong-Ren Chen
Keyword(s):  
Fluorescence Lifetime ◽  
Polymer Degradation ◽  
Fluorescence Technique ◽  
Fluorescence Lifetimes ◽  
Time Resolved Fluorescence ◽  
Time Resolved

The time-resolved fluorescence technique enables us to differentiate between polymer degradation and vesicle leakage by employing fluorescence lifetimes and their amplitudes.

scholarly journals CHARACTERIZING FLUORESCENCE LIFETIME OF NAD(P)H IN HUMAN LEUKEMIC MYELOID CELLS AND MONONUCLEAR CELLS

2013 ◽  
Vol 06 (04) ◽  
pp. 1350042
Author(s):  
LI-SHENG LIN ◽  
LI-NA LIU ◽  
HUI-FANG HUANG ◽  
YUAN-ZHONG CHEN ◽  
BU-HONG LI ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Mononuclear Cells ◽  
Relative Concentration ◽  
Myeloid Cells ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Fluorescence Lifetimes ◽  
Label Free ◽  
Time Resolved ◽  
Significant Difference ◽  
The Mean

The aim of this ex vivo study was to explore the potential of using the fluorescence lifetime of intracellular reduced nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) as a label-free indicator to characterize the differences between human leukemic myeloid cells and normal mononuclear cells (MNC). The steady-state and time-resolved autofluorescence of two human leukemic myeloid cell lines (K562, HL60) and MNC were measured by a spectrofluorimeter. According to excitation–emission matrix (EEM) analysis, the optimal emission of NAD(P)H in these cells suspensions occurred at 445 nm. Furthermore, the fluorescence lifetimes of NAD(P)H in leukemic myeloid cells and MNC were determined by fitting the time-resolved autofluorescence data. The mean fluorescence lifetimes of NAD(P)H in K562, HL60, and MNC cells were 5.57 ± 1.19, 4.45 ± 0.71, and 7.31 ± 0.60 ns, respectively. There was a significant difference in the mean lifetime of NAD(P)H between leukemic myeloid cells and MNC (p < 0.05). The difference was essentially caused by the change in relative concentration of free and protein-bound NAD(P)H. This study suggests that the mean fluorescence lifetime of NAD(P)H might be a potential label-free indicator for differentiating leukemic myeloid cells from MNC.

scholarly journals In Vivo Resolution of Multiexponential Decays of Multiple Near-Infrared Molecular Probes by Fluorescence Lifetime-Gated Whole-Body Time-Resolved Diffuse Optical Imaging

2007 ◽  
Vol 6 (4) ◽  
pp. 7290.2007.00020 ◽  
Author(s):  
Walter Akers ◽  
Frederic Lesage ◽  
Dewey Holten ◽  
Samuel Achilefu
Keyword(s):  
Optical Imaging ◽  
Fluorescence Lifetime ◽  
Fluorescence Intensity ◽  
Near Infrared ◽  
Molecular Probes ◽  
Whole Body ◽  
Diffuse Optical Imaging ◽  
Fluorescence Lifetimes ◽  
Time Resolved

The biodistribution of two near-infrared fluorescent agents was assessed in vivo by time-resolved diffuse optical imaging. Bacteriochlorophyll a (BC) and cypate-glysine-arginine-aspartic acid-serine-proline-lysine-OH (Cyp-GRD) were administered separately or combined to mice with subcutaneous xenografts of human breast adenocarcinoma and slow-release estradiol pellets for improved tumor growth. The same excitation (780 nm) and emission (830 nm) wavelengths were used to image the distinct fluorescence lifetime distribution of the fluorescent molecular probes in the mouse cancer model. Fluorescence intensity and lifetime maps were reconstructed after raster-scanning whole-body regions of interest by time-correlated single-photon counting. Each captured temporal point-spread function (TPSF) was deconvolved using both a single and a multiexponental decay model to best determine the measured fluorescence lifetimes. The relative signal from each fluorophore was estimated for any region of interest included in the scanned area. Deconvolution of the individual TPSFs from whole-body fluorescence intensity scans provided corresponding lifetime images for comparing individual component biodistribution. In vivo fluorescence lifetimes were determined to be 0.8 ns (Cyp-GRD) and 2 ns (BC). This study demonstrates that the relative biodistribution of individual fluorophores with similar spectral characteristics can be compartmentalized by using the time-domain fluorescence lifetime gating method.

scholarly journals Subcellular localization-dependent changes in EGFP fluorescence lifetime measured by time-resolved flow cytometry

2013 ◽  
Vol 4 (8) ◽  
pp. 1390 ◽  
Author(s):  
Ali Vaziri Gohar ◽  
Ruofan Cao ◽  
Patrick Jenkins ◽  
Wenyan Li ◽  
Jessica P. Houston ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Subcellular Localization ◽  
Fluorescence Lifetime ◽  
Time Resolved ◽  
Egfp Fluorescence

scholarly journals The biochemical resolving power of fluorescence lifetime imaging

2021 ◽  
Author(s):  
Andrew L. Trinh ◽  
Alessandro Esposito
Keyword(s):  
Fluorescence Lifetime ◽  
Resolving Power ◽  
Fluorescence Lifetime Imaging ◽  
Time Resolved ◽  
Instrument Response Function ◽  
Lifetime Imaging ◽  
Use Of Time ◽  
Single Living Cells ◽  
Microscopy Techniques

AbstractA deeper understanding of spatial resolution in microscopy fostered a technological revolution that is now permitting us to investigate the structure of the cell with nanometer resolution. Although fluorescence microscopy techniques enable scientists to investigate both the structure and biochemistry of the cell, the biochemical resolving power of a microscope is a physical quantity that is not well-defined or studied. To overcome this limitation, we carried out a theoretical investigation of the biochemical resolving power in fluorescence lifetime imaging microscopy, one of the most effective tools to investigate biochemistry in single living cells. With the theoretical analysis of information theory and Monte Carlo simulations, we describe how the ‘biochemical resolving power’ in time-resolved sensing depends on instrument specifications. We unravel common misunderstandings on the role of the instrument response function and provide theoretical insights that have significant practical implications in the design and use of time-resolved instrumentation.

Time-resolved acousto-optic interaction in single-mode optical fibers: characterization of axial nonuniformities at the nanometer scale

2014 ◽  
Vol 39 (6) ◽  
pp. 1437 ◽  
Author(s):  
E. P. Alcusa-Sáez ◽  
A. Díez ◽  
M. González-Herráez ◽  
M. V. Andrés
Keyword(s):  
Optical Fibers ◽  
Single Mode ◽  
Nanometer Scale ◽  
Time Resolved ◽  
Acousto Optic Interaction

Local Environment of Surface-Polyelectrolyte-Bound DNA Oligomers

2000 ◽  
Vol 651 ◽  
Author(s):  
Sangmin Jeon ◽  
Sung Chul Bae ◽  
Jiang John Zhao ◽  
Steve Granick
Keyword(s):  
Aqueous Solution ◽  
Salt Concentration ◽  
Fluorescence Anisotropy ◽  
Local Environment ◽  
Time Resolved Fluorescence ◽  
Surface Complexes ◽  
Dna Oligomers ◽  
Time Resolved ◽  
Two Photon ◽  
Anisotropy Decay

AbstractTwo-photon time-resolved fluorescence anisotropy methods were used to study the dynamical environment when fluorescent-labelled DNA oligomers (labelled with FAM, 6-fluorescein-6-carboxamido hexanoate) formed surface complexes with quaternized polyvinylpyridine (QPVP) cationic layers on a glass surface. We compared the anisotropy decay of DNA in bulk aqueous solution, DNA adsorbed onto QPVP, and QPVP-DNA-QPVP sandwich structures. When DNA was adsorbed onto QPVP, its anisotropy decay was dramatically retarded compared to the bulk, which means it had very slow rotational motion on the surface. Motions slowed down with increasing salt concentration up to a level of 0.1 M NaCl, but mobility began to increase at still higher salt concentration owing to detachment from the surface-immobilizing QPVP layers.

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